Emerging frontiers in detection and control of bacterial biofilms

Tan, Seth Yang-En; Chew, Su Chuen; Tan, Sean Yang-Yi; Givskov, Michael; Yang, Liang

doi:10.1016/j.copbio.2013.08.002

Cited by 83 publications

(60 citation statements)

References 41 publications

(44 reference statements)

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“…The adhesion of bacteria to surfaces results in the formation of biofilms, which are known to increase the resistance of the cells within them to environmental stress and antimicrobial compounds, when compared with free-living or planktonic cells. Bacteria living in biofilms can be a threat to human health (Tan et al 2014) as they may cause infections associated with indwelling devices, such as catheters and implants (Gomes et al 2014). Bacteria within biofilms may also cause a reduction in the efficiency of cooling water systems (Nagai et al 2013), spoil food in the food industry, reducing its shelf-life and facilitating the transmission of disease (Giaouris et al 2014) or reduce water quality in drinking water distribution systems (Liu et al 2013).…”

Section: Please Scroll Down For Articlementioning

confidence: 98%

Photodynamic inactivation induced by carboxypterin: a novel non-toxic bactericidal strategy against planktonic cells and biofilms ofStaphylococcus aureus

et al. 2015

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Microbial related contamination is of major concern and can cause substantial economic losses. Photodynamic inactivation (PDI) has emerged as a suitable approach to inhibit microorganism proliferation. In this work, PDI induced by 6-carboxypterin (Cap), a biocompatible photosensitizer (PS), was analyzed. The growth inhibition of Staphylococcus aureus exposed to artificial UV-A radiation and sunlight in the presence of Cap was investigated. After UV-A irradiation, 50 μM Cap was able to decrease by three orders (with respect to the initial value) the number of S. aureus cells in early biofilms. However, this concentration was 500 times higher than that needed for eradicating planktonic cells. Importantly, under solar exposure, 100 μM Cap was able to suppress sessile bacterial growth. Thus, this strategy is able to exert a bactericidal effect on sessile bacteria and to eradicate planktonic cells by exposing the Cap-containing sample to sunlight.

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Section: Please Scroll Down For Articlementioning

confidence: 98%

Photodynamic inactivation induced by carboxypterin: a novel non-toxic bactericidal strategy against planktonic cells and biofilms ofStaphylococcus aureus

et al. 2015

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“…35,36 Cross-contamination of foods from these persistent pathogen "reservoirs" is a major risk factor in the food processing environment with respect to the spread of food borne diseases within the consumer food chain. For example, contamination of food processing equipment with biofilms has been reported to be a contributing factor in 59% of food-borne disease outbreaks investigated in France.…”

Section: Discussionmentioning

confidence: 99%

A halotolerant thermostable lipase from the marine bacteriumOceanobacillussp. PUMB02 with an ability to disrupt bacterial biofilms

et al. 2014

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A halotolerant thermostable lipase was purified and characterized from the marine bacterium Oceanobacillus sp. PUMB02. This lipase displayed a high degree of stability over a wide range of conditions including pH, salinity, and temperature. It was optimally active at 30 °C and pH 8.0 respectively and was stable at higher temperatures (50-70 °C) and alkaline pH. The molecular mass of the lipase was approximately 31 kDa based on SDS-PAGE and MALDI-ToF fingerprint analysis. Conditions for enhanced production of lipase by Oceanobacillus sp. PUMB02 were attained in response surface method-guided optimization with factors such as olive oil, sucrose, potassium chromate, and NaCl being evaluated, resulting in levels of 58.84 U/ml being achieved. The biofilm disruption potential of the PUMB02 lipase was evaluated and compared with a marine sponge metagenome derived halotolerant lipase Lpc53E1. Good biofilm disruption activity was observed with both lipases against potential food pathogens such as Bacillus cereus MTCC1272, Listeria sp. MTCC1143, Serratia sp. MTCC4822, Escherichia coli MTCC443, Pseudomonas fluorescens MTCC1748, and Vibrio parahemolyticus MTCC459. Phase contrast microscopy, scanning electron microscopy, and confocal laser scanning microscopy showed very effective disruption of pathogenic biofilms. This study reveals that marine derived hydrolytic enzymes such as lipases may have potential utility in inhibiting biofilm formation in a food processing environment and is the first report of the potential application of lipases from the genus Oceanobacillus in biofilm disruption strategies.

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“…Furthermore, pathogenic bacteria in biofilms are more dangerous to human health than planktonic cells, since they are capable of resisting sanitizers and antibiotics. Bacterial biofilms may also cause a variety of problems in food processing due to disturbances of heat transfer, as well as leading to infections among consumers [59].…”

Section: Biofilms and Structured Amps On Solid Surfacesmentioning

confidence: 99%

Next-Generation Nanoantibacterial Tools Developed from Peptides

Vries

Andrade

Bakuzis

et al. 2015

Nanomedicine (Lond.)

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Bacteria resistant against various antimicrobial compounds have emerged in many countries, and the age of resistance has just started. Among the more promising novel antimicrobial compounds on which current research is focusing are the antimicrobial peptides (AMPs). These are often less susceptible to bacterial resistance since multiple modifications in the cellular membranes, cell wall and metabolism are required to reduce their effectiveness. Most likely, the use of pure AMPs will be insufficient for controlling pathogenic bacteria, and innovative approaches are required to employ AMPs in new antibiotic treatments. Therefore, here we review novel bionanotechnological approaches, including nanofibers, nanoparticles and magnetic particles for effectively using AMPs in fighting infectious diseases.

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Emerging frontiers in detection and control of bacterial biofilms

Cited by 83 publications

References 41 publications

Photodynamic inactivation induced by carboxypterin: a novel non-toxic bactericidal strategy against planktonic cells and biofilms ofStaphylococcus aureus

Photodynamic inactivation induced by carboxypterin: a novel non-toxic bactericidal strategy against planktonic cells and biofilms ofStaphylococcus aureus

A halotolerant thermostable lipase from the marine bacteriumOceanobacillussp. PUMB02 with an ability to disrupt bacterial biofilms

Next-Generation Nanoantibacterial Tools Developed from Peptides

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