Emerging Enteropathogenic<i>Escherichia coli</i>Strains?

Gomes, Tânia A. T.; Irino, Kinue; Girão, Dennys M.; Girao, Valeria B. C.; Guth, Beatriz E. C.; Vaz, Tânia; Moreira, Fabiana C.; Chinarelli, Silvia H.; Vieira, Mônica A. M.

doi:10.3201/eid1010.031093

Cited by 107 publications

(105 citation statements)

References 17 publications

(23 reference statements)

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“…Most tEPEC and aEPEC strains belong to defined (traditional) EPEC serogroups (34). Nevertheless, we previously identified and characterized several E. coli strains of non-EPEC serogroups isolated in three cities in Brazil carrying the intimin-encoding gene (eae) and lacking Shiga toxin-encoding genes (12). That study emphasized the large diversity of such strains but allowed no identification of the virulence potential of individual serotypes.…”

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“…These results were compiled with those of our previous study (12) to attain an overview of the virulence potential of serotype O51:H40. Table 1 presents the origins and the clinical and microbiological data of the patients carrying the 11 O51:H40 strains studied (7,12,13). Their adherence patterns in HeLa and differentiated intestinal Caco-2 cells were determined previously (12,36), and in this study, strains were further tested in differentiated T84 cells as described previously (1).…”

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“…Table 1 presents the origins and the clinical and microbiological data of the patients carrying the 11 O51:H40 strains studied (7,12,13). Their adherence patterns in HeLa and differentiated intestinal Caco-2 cells were determined previously (12,36), and in this study, strains were further tested in differentiated T84 cells as described previously (1). In HeLa cells, strain 0151-1 expressed LA after 3 h of infection, whereas seven strains presented an LA-like (LAL) pattern (27), and three strains showed a noncharacteristic pattern in prolonged (6-h) assays (12,36).…”

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confidence: 99%

“…In this study, we examined all 10 strains of serotype O51: H40, the most frequently found serotype in our previous study (12), and an additional uncharacterized O51:H40 strain (7) for novel E. coli virulence properties, their enteropathogenic potentials in vivo, genetic diversity, and genetic relatedness to tEPEC and EHEC. These results were compiled with those of our previous study (12) to attain an overview of the virulence potential of serotype O51:H40.…”

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Escherichia coli Strains of Serotype O51:H40 Comprise Typical and Atypical Enteropathogenic E. coli Strains and Are Potentially Diarrheagenic

et al. 2008

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Escherichia coli strains of serotype O51:H40 were studied with regard to the presence of several virulence properties and their genetic diversity and enteropathogenicity in rabbit ileal loops. This serotype encompasses potential enteropathogenic strains mostly classified as being atypical enteropathogenic E. coli (EPEC) strains, which are genetically closer to enterohemorrhagic E. coli than to typical EPEC strains.Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) comprise two of the six diarrheagenic E. coli pathotypes (16). While EHEC secretes Shiga toxin(s), EPEC adheres to HeLa/HEp-2 cells, forming compact clusters that distinguish the localized pattern of adherence (LA) (16). The LA is mediated by the bundle-forming pilus encoded by the EPEC adherence factor plasmid (pEAF) (16).EHEC and EPEC share the ability to promote attachingeffacing (AE) lesions in intestinal cells (11) characterized by intimate bacterial adherence, localized microvillus effacement, and accumulation of polymerized actin and other cytoskeleton elements, resulting in pedestal-like structures underneath adherent bacteria (11). The AE lesion-associated genes reside in the locus of enterocyte effacement (LEE) (20), a pathogenicity island encoding a type III secretion system, regulators, chaperones, and effectors that interfere with diverse cell signaling processes (revised in reference 11). The LEE also encodes the outer membrane adhesive protein intimin (15) and its translocated receptor, Tir (18).EPEC is currently subgrouped into typical EPEC (tEPEC) and atypical EPEC (aEPEC), where pEAF is present only in the former group (17, 34). Most tEPEC and aEPEC strains belong to defined (traditional) EPEC serogroups (34). Nevertheless, we previously identified and characterized several E. coli strains of non-EPEC serogroups isolated in three cities in Brazil carrying the intimin-encoding gene (eae) and lacking Shiga toxin-encoding genes (12). That study emphasized the large diversity of such strains but allowed no identification of the virulence potential of individual serotypes. Moreover, as both diarrheic and nondiarrheic patients may carry aEPEC strains (34,36), it is important to evaluate the virulence potential of selected strains to identify those strains that are truly enteropathogenic.In this study, we examined all 10 strains of serotype O51: H40, the most frequently found serotype in our previous study (12), and an additional uncharacterized O51:H40 strain (7) for novel E. coli virulence properties, their enteropathogenic potentials in vivo, genetic diversity, and genetic relatedness to tEPEC and EHEC. These results were compiled with those of our previous study (12) to attain an overview of the virulence potential of serotype O51:H40. Table 1 presents the origins and the clinical and microbiological data of the patients carrying the 11 O51:H40 strains studied (7,12,13).

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Escherichia coli Strains of Serotype O51:H40 Comprise Typical and Atypical Enteropathogenic E. coli Strains and Are Potentially Diarrheagenic

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Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae

et al. 2016

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Atypical enteropathogenic Escherichia coli (aEPEC) are heterogeneous strains in terms of serotypes, adherence patterns and the presence of novel virulence factors. This heterogeneity is intriguing, promoting studies trying to characterize these novel proteins and to better comprehend this pathotype group. In a previous study analyzing low‐molecular mass proteomes of four representative aEPEC strains of three different adhesion phenotypes, we classified proteins according to their annotated function, with most of them being involved in metabolism and transport; while some of them were classified as hypothetical proteins. The majority of the hypothetical proteins were homologue products of genes identified in the genome of enterohemorrhagic E. coli. One of the hypothetical proteins was annotated as Z2335, with orthologue in EPEC, and by bioinformatics analysis, this protein was revealed to be the universal stress protein F (UspF). Thus, herein we successfully obtained a recombinant UspF protein from aEPEC, which is a α/β, ATP‐binding protein involved in stress response, with comparable protein production among the four studied strains, but showing noteworthy differences when cultivated in different stress conditions, also present in other enterobacterial species, such as Shigella sonnei and Citrobacter freundii. Furthermore, our results confirm that the Usp protein superfamily encompasses a conserved group of proteins involved in stress resistance in aEPEC and other Enterobacteriaceae.

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Detection and Typing Strategies for Pathogenic Escherichia coli

Rivas¹

2015

SpringerBriefs in Food, Health, and Nutrition

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Pages: vi + 258 ISBN: Book: 978-1-910190-77-7. Ebook: 978-1-910190-78-4 £159, $319 Published by: Caister Academic Press www.caister.com Escherichia coli is an important member of the normal healthy microbiome of humans and other mammals. In addition, some strains are thought to be probiotic, and therefore beneficial to the host. However, other strains of E. coli have evolved into highly versatile, and frequently deadly, pathogens, the resultant diseases causing significant economic loss and public health burdens worldwide. Recent studies have shown that the E. coli genome has a high plasticity allowing it to adapt to new niches and survive in stressful conditions and to evolve into new hybrid strains with shared genes, including virulence genes. Omics and whole genome sequencing approaches have transformed research in this field allowing fascinating new insights into the molecular and cellular biology of the bacterium thus paving the way for the development of novel therapeutic strategies. Under the expert guidance of the editors in this book, renowned international authors provide timely and up-to-date reviews of current cutting-edge E. coli omics, molecular-and cellular-biology research. Topics range from E. coli genome plasticity and evolution to the application of omics technologies for in silico modeling to understand stress-triggered physiological responses. This authoritative volume is essential reading for scientists, both experts and students, working on pathogenic E. coli in academia, government, and biotechnology companies. It is also a must-read for anyone with an interest in bacterial pathogenesis and an important acquisition for all microbiology libraries.

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Emerging EnteropathogenicEscherichia coliStrains?

Cited by 107 publications

References 17 publications

Escherichia coli Strains of Serotype O51:H40 Comprise Typical and Atypical Enteropathogenic E. coli Strains and Are Potentially Diarrheagenic

Escherichia coli Strains of Serotype O51:H40 Comprise Typical and Atypical Enteropathogenic E. coli Strains and Are Potentially Diarrheagenic

Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae

Detection and Typing Strategies for Pathogenic Escherichia coli

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