Emerging contrast agents for multispectral optoacoustic imaging and their biomedical applications

Abstract

This tutorial review covers the introduction to multispectral optoacoustic imaging, emerging contrast agents, and their preclinical and clinical applications.

A H2O2-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging

“…If only AIE-active NIR-II fluorophores based on benzothiadiazole core can be converted into biomarker-activatable probes, the probes would perceptibly hold a great potential for more extensive applications including clinical translation. Optoacoustic (photoacoustic) imaging is an emerging modality that collects ultrasound waves generated by photoexciting contrast agents in tissues and produces images with high resolution and penetration depth [37][38][39][40][41][42][43][44] . A dual-mode probe applicable in both NIR-II fluorescence and optoacoustic imaging is highly advantageous because it provides mutually corroborated information.…”

Targeted and activatable nanosystem for fluorescent and optoacoustic imaging of immune-mediated inflammatory diseases and therapy via inhibiting NF-κB/NLRP3 pathways

“…Since multispectral optoacoustic tomography (MSOT) imaging system can generate cross-sectional 2D images using tomography technique and render a stack of 2D images into orthogonal-view 3D images, which are conducive to providing 3D information of disease site [ [57] , [58] , [59] , [60] , [61] , [62] , [63] ]. To accurately locate the AIH site and estimate the size of the disease site in mice, we performed MSOT imaging and presented the results in Fig.…”

“…Near infrared (NIR) fluorescence imaging technology has been established as a powerful tool for providing a high degree of sensitivity in biomedical applications due to its attractive properties such as high contrast and sensitivity, low cost, absence of ionizing radiation, ease of use, and high specificity [ [50] , [51] , [52] , [53] , [54] , [55] , [56] ]. Recently, to further improve the performance of the optical imaging, such as the elevation of penetration depth and reduction of tissue scattering, the optoacoustic imaging has emerged as a robust and promising modality for live animal imaging [ [57] , [58] , [59] , [60] , [61] , [62] , [63] ]. In particular, multispectral optoacoustic tomography (MSOT), which functions by illuminating a sample with NIR laser pulses of multiple wavelengths and collecting the ultrasound signals emitted by various photoabsorbers in the sample, can acquire wavelength-specific optoacoustic spectrum for a particular absorber and achieve both tomographic and three-dimensional images, which ensures accurate pinpointing of disease site in a spatiotemporal and non-invasive manner [ [57] , [58] , [59] , [60] , [61] , [62] , [63] ].…”

An Adenovirus‐Mimicking Photoactive Nanomachine Preferentially Invades and Destroys Cancer Cells through Hijacking Cellular Glucose Metabolism

“…Photoacoustic imaging is a novel imaging technique with the advantages of high spatial resolution and deep tissue imaging ability. [ 35 ] Usually, photothermal materials are effective photoacoustic imaging agents, suggesting that AMN is a potent candidate. Figure 5f shows that photoacoustic signals of AMN exhibited a dose‐dependent manner, and increased with increasing concentrations.…”