Emerging biosensing and transducing techniques for potential applications in point-of-care diagnostics

Qin, Junjie; Wang, Wei; Gao, Liqian; Yao, Shao Q.

doi:10.1039/d1sc06269g

Cited by 57 publications

(32 citation statements)

References 160 publications

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“…Successful demonstration of the PRISM assay for detecting various biomolecules in different sample matrices validates the methods of leveraging nanomechanical perturbations in Raman spectroscopy to design versatile biomarker detection platform. Furthermore, in light of recent advances on pointof-care devices, [41][42][43][44] and given the 2D nature of the plasmonic substrates used, the PRISM assay holds great promise to be seamlessly integrated into either paper-or plastic-based lateral flow devices for field-deployable applications.…”

Section: Detection Of Small Moleculesmentioning

confidence: 99%

Leveraging Nanomechanical Perturbations in Raman Spectro‐Immunoassays to Design a Versatile Serum Biomarker Detection Platform

Zheng

Raj

et al. 2022

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While immunoassays are pivotal to medical diagnosis and bioanalytical chemistry, the current landscape of public health has catalyzed an important shift in the requirements of immunoassays that demand innovative solutions. For example, rapid, label‐free, and low‐cost screening of a given analyte is required to inform the best countermeasures to combat infectious diseases in a timely manner. Yet, the current design of immunoassays cannot accommodate such requirements as constraint by accumulative challenges, such as repeated incubation and washing, and the need of two types of antibodies in the sandwich format. To provide a potential solution, herein, a plasmonic Raman immunoassay with single‐antibody, multivariate regression, and shift‐of‐peak strategies, coined as the PRISM assay, for serum biomarkers detection, is reported. The PRISM assay relies on Raman reporter‐antibody conjugates to capture analytes on a plasmonic substrate. The ensuing nanomechanical perturbations to vibration of Raman reporters induce subtle but characteristic spectral changes that encode rich information related to the captured analytes. By fusing Raman spectroscopy and chemometric analysis, both Raman frequency shift‐ and multivariate regression models for sensitive detection of biomarkers are developed. The PRISM assay is expected to find a wide range of applications in clinical diagnosis, food safety surveillance, and environmental monitoring.

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Section: Detection Of Small Moleculesmentioning

confidence: 99%

Leveraging Nanomechanical Perturbations in Raman Spectro‐Immunoassays to Design a Versatile Serum Biomarker Detection Platform

Zheng

Raj

et al. 2022

Small

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“…Consequently, most CRISPR/Cas-based assays still need additional nucleic acid amplification (e.g., recombinase polymerase amplification, RPA), thus inevitably bringing many problems such as complicated operations, long detection time, extra expenditure, nonspecific amplification, and cross-contamination. Moreover, the CRISPR/Cas system has been integrated into different detection platforms such as fluorescence, − electrochemistry, − colorimetry, − and Raman, , but, again, few of them can detect targets at the single-molecule level without amplification procedures. , Thus, it is highly desirable to develop an amplification-free, CRISPR/Cas-based platform for detecting nucleic acids at the single-molecule level.…”

Section: Introductionmentioning

confidence: 99%

Amplification-Free, Single-Microbead-Based Cas12a Assay for One-Step DNA Detection at the Single-Molecule Level

Yang

Zhang

et al. 2022

Anal. Chem.

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CRISPR/Cas-based systems are highly attractive for developing next-generation diagnostic technologies because of their intrinsic merits such as simplicity, sensitivity, and specificity. However, currently, nucleic acid amplification procedures are still needed to achieve attomolar sensitivity in most CRISPR/Cas-based assays, which causes high cost, operation difficulty, and low efficiency. Herein, we combine the CRISPR/Cas12a-based assay and a single-microbead detection platform for one-step and amplification-free detection of DNA at the single-molecule level. By modifying DNA reporters on a biomimetic membrane-coated microbead, the activated Cas12a by targets will cleave these reporters and lighten the bead within 10 min. The method allows the detection of the target down to three copies in a 5 μL sample. Furthermore, we successfully apply this method for the specific identification of viral infection, foodborne bacteria, and DNA mutation in real samples without extra nucleic acid amplification. We believe that this approach offers new insights for developing CRISPR/Cas-based DNA assays in biomedical applications.

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“…Various detection methods have been developed ranging from atomic absorption and emission spectroscopy to inductively coupled plasma mass spectrometry (ICP-MS), though most of them require large instruments, complex protocols, and high costs . Electrochemistry is a facile method for the detection of heavy metals with high sensitivity, low cost, fast response, and simplicity, drawing enormous attention in the field of environmental analysis. − Especially in Cu 2+ detection, mesoporous composite material due to its large electroactive surface area was successfully used for Cu 2+ capturing and sensing, achieving a limit of detection (LOD) of 0.33 μg/L; as the recognizing layer, conducting polyamide, chitosan (CS), or benzaldehyde was modified onto electrochemical sensors, bringing about an outstanding specificity and detection range. − However, electrochemical sensors designed for fresh water are usually inapplicable in seawater, mainly because the high salinity and complex redox components can generate false positive/negative signals, thus inflicting a major penalty to the sensitivity and specificity of the sensors. , Therefore, new electrochemical methods are in urgent need to overcome the strong matrix effect of seawater.…”

Section: Introductionmentioning

confidence: 99%

Dual-Modulated Heterojunctions for Anti-Interference Sensing of Heavy Metals in Seawater

Pang

et al. 2022

Anal. Chem.

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Trace analyte detection in a complex environment such as in seawater is usually challenging for classic redox-based electrochemical sensors since the matrix effect of high salinity and various interfering species with similar redox properties can generate false positive/negative signals, thus impacting the sensitivity and specificity of the sensors. In this work, unlike redox-based approaches, we propose a novel sensing mode that relies on dual-modulated interfacial energy barriers of heterojunctions. By constructing the hierarchical structure of Ni/TiO2/porous-reduced graphene oxide/chitosan (CS), we introduce interfacial energy barriers of Schottky junctions into the electrochemical sensors for Cu2+. Most importantly, we found that two factors, light and the electrostatic interactions between the heterojunctions and Cu2+, can be coupled to regulate the height of the interfacial energy barrier and at last exponentially magnify the sensing signals in response to Cu2+. Since the electrostatic interaction is inert to redox, the proposed sensor is robust against most interfering species even in seawater. Illumination further enhances its sensitivity by 6.23 times and endows it a limit of detection of 0.22 nM. Such a dual-modulated sensing mode is also valid in other heterojunctions such as in the p–n junctions of Ni/NiO/MoS2/CS, demonstrating its potential in more universal applications.

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Emerging biosensing and transducing techniques for potential applications in point-of-care diagnostics

Abstract: With the deepening of our understanding in life science, molecular biology, nanotechnology, optics, electrochemistry and other areas, an increasing number of biosensor design strategies have emerged in recent years, capable...

Cited by 57 publications

References 160 publications

Leveraging Nanomechanical Perturbations in Raman Spectro‐Immunoassays to Design a Versatile Serum Biomarker Detection Platform

Leveraging Nanomechanical Perturbations in Raman Spectro‐Immunoassays to Design a Versatile Serum Biomarker Detection Platform

Amplification-Free, Single-Microbead-Based Cas12a Assay for One-Step DNA Detection at the Single-Molecule Level

Dual-Modulated Heterojunctions for Anti-Interference Sensing of Heavy Metals in Seawater

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