Emerging and reemerging arboviruses: A new threat in Eastern Peru

Alva-Urcia, Carlos; Aguilar-Luis, Miguel Ángel; Palomares-Reyes, Carlos; Silva-Caso, Wilmer; Suárez-Ognio, Luis; Weilg, Pablo; Manrique, Carlos; Vasquez-Achaya, Fernando; Valle, Luís J. del; Valle-Mendoza, Juana del

doi:10.1371/journal.pone.0187897

Cited by 39 publications

(37 citation statements)

References 24 publications

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“…After collection, all the samples were stored at -80°C and transported to Lima (Peru) under standardized frozen conditions to perform molecular assays. In a previous study [ 25 ], in these samples we detect the presence of dengue, chikungunya, Zika and oropouche.…”

Section: Methodsmentioning

confidence: 93%

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Molecular detection and clinical characteristics of Bartonella bacilliformis, Leptospira spp., and Rickettsia spp. in the Southeastern Peruvian Amazon basin

et al. 2018

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BackgroundAcute febrile illness (AFI) represent a significant health challenge in the Peruvian Amazon basin population due to their diverse etiologies and the unavailability of specific on-site diagnostic methods, resulting in underreporting of cases. In Peru, one of the most endemic regions to dengue and leptospirosis is Madre de Dios, a region also endemic to emergent bacterial etiologic agents of AFI, such as bartonellosis and rickettsiosis, whose prevalence is usually underreported.We aimed to molecularly identify the presence of Leptospira spp., Bartonella bacilliformis, and Rickettsia spp. by Polymerase Chain Reaction in serum samples from patients with AFI from Puerto Maldonado-Madre de Dios in Peru.MethodsSerum samples from patients with acute febrile illness were analyzed by real-time PCR for detecting the presence of Bartonella bacilliformis, Leptospira spp. and Rickettsia spp.ResultsBartonella bacilliformis was the most prevalent bacteria identified in 21.6% (30/139) of the samples, followed by Leptospira spp. in 11.5% (16/139) and Rickettsia spp. in 6.5% (9/139) of the samples. No co-infections were observed between these bacteria. The most frequent symptoms associated with fever among all groups, were headaches, myalgias, and arthralgias. We found no statistically significant differences in the clinical presentation between patients infected with each bacterium.ConclusionsIn a previous study, we shown the presence of dengue, chikungunya, Zika and oropouche virus. We were able to identify these pathogens in 29.5% of all the samples, with chikungunya and OROV as the most frequently found in 9.4 and 8.6% of all the samples, respectively. In this study we show that B. bacilliformis (21.6%), Leptospira spp. (11.5%) and Rickettsia spp. (6.5%) accounted for the main etiologies of AFI in samples from Puerto Maldonado-Madre de Dios, Perú. Our analysis of their clinical presentation, further shows the importance of implementing more sensitive and specific on-site diagnostic tools in the national surveillance programs.This study confirms that the un-specificity of signs and symptoms is not only associated with arboviral infections, but also with the clinical presentation of endemic bacterial infections.

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Section: Methodsmentioning

confidence: 93%

“…In a previous study [ 25 ], we aimed to molecularly detect the presence of dengue, chikungunya, Zika and oropouche virus among patients with AFI in Madre de Dios. We were able to identify these pathogens in 29.5% of all the samples, with chikungunya and OROV as the most frequently found in 9.4% and 8.6% of all the samples, respectively.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and clinical characteristics of Bartonella bacilliformis, Leptospira spp., and Rickettsia spp. in the Southeastern Peruvian Amazon basin

et al. 2018

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“…The infection with Oropouche virus can result in an acute febrile and exanthematous illness, with symptoms frequently related to other viral infections such as dengue. Oropouche fever cases were reported in several Brazilian states including Amazonas, Acre, Pará, and Mato Grosso, as well as in other South America countries (2–9) .…”

Section: Textmentioning

confidence: 99%

Oropouche virus detection in saliva and urine

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Santos

Monteiro

et al. 2019

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Oropouche virus (OROV) is an arthropod-borne virus of the Peribunyaviridae family, transmitted to humans primarily by Culicoides paraensis. It is one of the main arboviruses that infect humans in Brazil, mainly in the Amazon region. We report the OROV detection in the saliva and urine five days after the symptom’s onset. Results were further confirmed by nucleotide sequencing and phylogenetic analysis. To our knowledge, this is the first report that OROV may be detected in the saliva and urine of infected patients. In addition, our results may contribute to the current knowledge regarding the natural history of Oropouche fever.

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“…A one-step RT-PCR was performed as described by Alva et al [ 26 ]. The primers and the probe used are the ones described by Faye et al [ 27 ].…”

Section: Main Textmentioning

confidence: 99%

Detection of Zika virus infection among asymptomatic pregnant women in the North of Peru

et al. 2018

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ObjectiveTo report an outbreak of ZIKV infection among asymptomatic pregnant women during 2016 in the city of Jaen, Cajamarca.ResultsZika virus RNA was detected in 3.2% (n = 36) of cases by RT-PCR. The mean age of patients positive for ZIKV infection was 29.6 years. 7 patients (19.4%) infected with ZIKV were in their first-trimester of gestation, 13 (36.1%) were in their second-trimester, and 16 (44%) were in their third-trimester. All of the infected pregnant women were asymptomatic. ZIKV infection remains a major public health issue that calls for constant epidemiological surveillance. It can cause the congenital Zika virus syndrome in the newborns of infected mothers. The lack of molecular diagnostic methods in isolated localities and the similarity of symptoms to other arboviral infections, lead to an under-diagnosis of this disease in endemic areas.

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Emerging and reemerging arboviruses: A new threat in Eastern Peru

Cited by 39 publications

References 24 publications

Molecular detection and clinical characteristics of Bartonella bacilliformis, Leptospira spp., and Rickettsia spp. in the Southeastern Peruvian Amazon basin

Molecular detection and clinical characteristics of Bartonella bacilliformis, Leptospira spp., and Rickettsia spp. in the Southeastern Peruvian Amazon basin

Oropouche virus detection in saliva and urine

Detection of Zika virus infection among asymptomatic pregnant women in the North of Peru

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