Emergence of uncommon KL38-OCL6-ST220 carbapenem-resistant Acinetobacter pittii strain, co-producing chromosomal NDM-1 and OXA-820 carbapenemases

Tian, Chongmei; Xing, Mengyu; Fu, Liping; Zhao, Yaping; Fan, Xueyu; Wang, Siwei

doi:10.3389/fcimb.2022.943735

Cited by 14 publications

(11 citation statements)

References 66 publications

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“…Virulence factors were predicted using ABRicate (139,140) v1.0.1 (https://github.com/tseemann/ABRicate). The default virulence factor database included in ABRicate was replaced with DNA sequences from the full dataset to include all genes related to known and predicted virulence factors (141), (http://www.mgc.ac.cn/VFs/download.html).…”

Section: Methodsmentioning

confidence: 99%

Establishment and comparative genomics of a high-quality collection of mosquito-associated bacterial isolates - MosAIC (Mosquito-Associated Isolate Collection)

Foo,

Brettell,

Nichols

et al. 2023

Preprint

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Mosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such asPlasmodiumspp., the causative agent of malaria. Mosquito microbiomes are critically important for the ability of mosquitoes to transmit disease-causing agents. However, while large collections of bacterial isolates and genomic data exist for vertebrate microbiomes, the vast majority of work in mosquitoes to date is based on 16S rRNA gene amplicon data that provides limited taxonomic resolution and no functional information. To address this gap and facilitate future studies using experimental microbiome manipulations, we generated a bacterialMosquito-Associated IsolateCollection (MosAIC) consisting of 392 bacterial isolates with extensive metadata and high-quality draft genome assemblies that are publicly available for use by the scientific community. MosAIC encompasses 142 species spanning 29 bacterial families, with members of theEnterobacteriaceaecomprising 40% of the collection. Phylogenomic analysis of three genera,Enterobacter, Serratia, andElizabethkingia, reveal lineages of mosquito-associated bacteria isolated from different mosquito species in multiple laboratories. Investigation into species’ pangenomes further reveals clusters of genes specific to these lineages, which are of interest for future work to identify functions underlying mosquito host association. Altogether, we describe the generation of a physical collection of mosquito-associated bacterial isolates, their genomic data, and analyses of selected groups in context of genome data from closely related isolates, providing a unique, highly valuable resource to investigate factors for bacterial colonisation and adaptation within mosquito hosts. Future efforts will expand the collection to include broader geographic and host species representation, especially from individuals collected from field populations, as well as other mosquito-associated microbes, including fungi, archaea, and protozoa.

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Section: Methodsmentioning

confidence: 99%

Establishment and comparative genomics of a high-quality collection of mosquito-associated bacterial isolates - MosAIC (Mosquito-Associated Isolate Collection)

Foo,

Brettell,

Nichols

et al. 2023

Preprint

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“…Compared to A. baumannii , few studies have focused on A. nosocomialis and A. pittii in recent decades owing to their lower prevalence and resistance rates. However, they have garnered increasing research attention recently owing to a rise in antibiotic resistance and changes in resistance mechanisms, particularly in A. pittii strains ( Singkham-In and Chatsuwan, 2018 ; Chen et al., 2019 ; Zhang et al., 2020 ; Yang et al., 2021 ; Ding et al., 2022 ; Tian et al., 2022 ). Additionally, a recent multicenter investigation in Japan indicated that A. pittii is the most significant species responsible for invasive Acinetobacter infections ( Kiyasu et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of the capsule depolymerase Dpo27 from phage IME-Ap7 specific to Acinetobacter pittii

Wang,

Liu,

Zhang

et al. 2024

Front. Cell. Infect. Microbiol.

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Among the Acinetobacter genus, Acinetobacter pittii stands out as an important opportunistic infection causative agent commonly found in hospital settings, which poses a serious threat to human health. Recently, the high prevalence of carbapenem-resistant A. pittii isolates has created significant therapeutic challenges for clinicians. Bacteriophages and their derived enzymes are promising therapeutic alternatives or adjuncts to antibiotics effective against multidrug-resistant bacterial infections. However, studies investigating the depolymerases specific to A. pittii strains are scarce. In this study, we identified and characterized a capsule depolymerase, Dpo27, encoded by the bacteriophage IME-Ap7, which targets A. pittii. A total of 23 clinical isolates of Acinetobacter spp. were identified as A. pittii (21.91%, 23/105), and seven A. pittii strains with various K locus (KL) types (KL14, KL32, KL38, KL111, KL163, KL207, and KL220) were used as host bacteria for phage screening. The lytic phage IME-Ap7 was isolated using A. pittii 7 (KL220) as an indicator bacterium and was observed for depolymerase activity. A putative tail fiber gene encoding a polysaccharide-degrading enzyme (Dpo27) was identified and expressed. The results of the modified single-spot assay showed that both A. pittii 7 and 1492 were sensitive to Dpo27, which was assigned the KL220 type. After incubation with Dpo27, A. pittii strain was susceptible to killing by human serum; moreover, the protein displayed no hemolytic activity against erythrocytes. Furthermore, the protein exhibited sustained activity across a wide pH range (5.0–10.0) and at temperatures between 20 and 50°C. In summary, the identified capsule depolymerase Dpo27 holds promise as an alternative treatment for combating KL220-type A. pittii infections.

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“… 12 Closely related plasmids were predicted using the Bacterial Isolate Genome Sequence Database (BIGSdb). 13 …”

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confidence: 99%

Emergence of a ST248Pasteur-ST1068Oxford Carbapenem Resistance Acinetobacter pittii Clinical Isolate in China, Co-Harboring OXA-58 and OXA-500 Carbapenemases

Tian,

Song,

Huang

et al. 2023

IDR

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Emergence of uncommon KL38-OCL6-ST220 carbapenem-resistant Acinetobacter pittii strain, co-producing chromosomal NDM-1 and OXA-820 carbapenemases

Cited by 14 publications

References 66 publications

Establishment and comparative genomics of a high-quality collection of mosquito-associated bacterial isolates - MosAIC (Mosquito-Associated Isolate Collection)

Establishment and comparative genomics of a high-quality collection of mosquito-associated bacterial isolates - MosAIC (Mosquito-Associated Isolate Collection)

Identification and characterization of the capsule depolymerase Dpo27 from phage IME-Ap7 specific to Acinetobacter pittii

Emergence of a ST248Pasteur-ST1068Oxford Carbapenem Resistance Acinetobacter pittii Clinical Isolate in China, Co-Harboring OXA-58 and OXA-500 Carbapenemases

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