Emergence of tomato leaf curl New Delhi virus in Italy: estimation of incidence and genetic diversity

Panno, Stefano; Caruso, A.; Troiano, Elisa; Luigi, M.; Manglli, Ariana; Vatrano, T.; Iacono, Giuseppe; Marchione, S.; Bertin, Sabrina; Tomassoli, L.; Parrella, G.; Davino, Salvatore

doi:10.1111/ppa.12978

Cited by 37 publications

(55 citation statements)

References 31 publications

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“…In the last years, emerging viral diseases have been an important limiting factor for many crop production systems, such as tomato crops, causing considerable economic losses (Hanssen, Lapidot & Thomma, 2010). Greenhouse tomato production in Italy is affected by important losses that are caused by several different viruses: Pepino mosaic virus (Davino et al, 2017b, 2008; Tiberini et al, 2011), Tomato mosaic virus (ToMV), Tomato spotted wilt virus (Panno et al, 2012), Tomato leaf curl New Delhi virus (Panno et al, 2019), Tomato yellow leaf curl virus—Tomato yellow leaf curl Sardinia virus and their recombinants (Davino et al, 2009, 2012; Panno, Caruso & Davino, 2018) and Tomato brown rugose fruit virus (ToBRFV) detected at the end of 2018 (Panno, Caruso & Davino, 2019).…”

Section: Introductionmentioning

confidence: 99%

Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques

Panno

Ruiz-Ruiz

Caruso

et al. 2019

PeerJ

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Background Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops. In Italy, the first ToBRFV outbreak occurred in 2018 in several provinces of the Sicily region. ToBRFV outbreak represents a serious threat for tomato crops in Italy and the Mediterranean Basin. Methods Molecular and biological characterisation of the Sicilian ToBRFV ToB-SIC01/19 isolate was performed, and a sensitive and specific Real-time RT-PCR TaqMan minor groove binder probe method was developed to detect ToBRFV in infected plants and seeds. Moreover, four different sample preparation procedures (immunocapture, total RNA extraction, direct crude extract and leaf-disk crude extract) were evaluated. Results The Sicilian isolate ToB-SIC01/19 (6,391 nt) showed a strong sequence identity with the isolates TBRFV-P12-3H and TBRFV-P12-3G from Germany, Tom1-Jo from Jordan and TBRFV-IL from Israel. The ToB-SIC01/19 isolate was successfully transmitted by mechanical inoculations in S. lycopersicum L. and Capsicum annuum L., but no transmission occurred in S. melongena L. The developed real-time RT-PCR, based on the use of a primer set designed on conserved sequences in the open reading frames3, enabled a reliable quantitative detection. This method allowed clear discrimination of ToBRFV from other viruses belonging to the genus Tobamovirus, minimising false-negative results. Using immunocapture and total RNA extraction procedures, the real-time RT-PCR and end-point RT-PCR gave the same comparable results. Using direct crude extracts and leaf-disk crude extracts, the end-point RT-PCR was unable to provide a reliable result. This developed highly specific and sensitive real-time RT-PCR assay will be a particularly valuable tool for early ToBRFV diagnosis, optimising procedures in terms of costs and time.

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Section: Introductionmentioning

confidence: 99%

Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques

Panno

Ruiz-Ruiz

Caruso

et al. 2019

PeerJ

Self Cite

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“…the pest has to be eradicated immediately if detected. However, the Panel notices the frequent report of outbreaks in Spain since 2013 (Table 3), and information from the literature suggesting that it might be more widespread in open field and in the environment than officially reported (Ju arez et al, 2019;Panno et al, 2019). 2017Source: EPPO GD, accessed on 24/2/2020.…”

Section: Pest Distribution In the Eumentioning

confidence: 99%

Pest categorisation of tomato leaf curl New Delhi virus

Bragard¹,

Dehnen‐Schmutz²,

Serio³

et al. 2020

EFS2

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Following a request from the European Commission, the Panel on Plant Health performed a pest categorisation on tomato leaf curl New Delhi virus (ToLCNDV). ToLCNDV is a well‐defined bipartite Begomovirus species, sometimes associated with satellite molecules. It is transmitted by Bemisia tabaci to a wide range of hosts. ToLCNDV is reported from Estonia, Greece, Italy, Portugal and Spain, with limited distribution. The prevalent strain (ToLCNDV‐ES) in these countries is particularly adapted to cucurbits and is different from isolates reported outside the EU, which are better adapted to solanaceous crops and could therefore pose additional risk for EU agriculture. The virus is regulated under Commission Implementing Regulation (EU) 2019/2072. The main pathway of entry identified is plants for planting of susceptible hosts, even if entry could also occur via commodities carrying viruliferous B. tabaci and possibly by seeds. While establishment and local spread rely on B. tabaci, the virus can also be dispersed over long distances by movement of infected plants for planting. Establishment and spread are limited to regions with ecoclimatic conditions suitable for the establishment of vector populations (southern regions of Europe) or can occur as outbreaks wherever crops are grown under protected cultivation. The main uncertainties associated with this pest categorisation are the distribution and prevalence of ToLCNDV in the EU, the magnitude of the virus impact particularly on hosts different from Cucurbitaceae, and seed transmission. ToLCNDV meets all the criteria evaluated by EFSA to qualify as potential Union Quarantine Pest (QP); conversely, ToLCNDV does not meet the criterion of being widespread in the EU to qualify as a Regulated Non‐Quarantine Pest (RNQP). Should new data show that ToLCNDV is widespread in the EU, the possibility would exist for non‐EU isolates to qualify as QP, while ToLCNDV EU isolates (ToLCNDV‐ES) could qualify as RNQP.

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“…The genomes of ToLCNDV isolates are most diverse in Asia (Moriones et al, 2017) and as shown for many other geminiviruses, the genomes are shaped by recombination events comprising sequences of 1 The Pest Survey Card will be updated in the form of Story Map that will be available in the Plant Pests Story Maps Gallery available online: https://efsa.maps.arcgis.com/apps/MinimalGallery/index.html?appid=f91d6e95376f4a5da206eb1815ad1489 2 https://zenodo.org/record/2541541/preview/ribess-manual.pdf 3 https://shiny-efsa.openanalytics.eu/ other begomoviruses or sequences of unknown origin (Lefeuvre and Moriones, 2015). In contrast to the diversity of ToLCNDV genomes reported from India and Pakistan, the ToLCNDV isolates that are currently found in the EU are genetically uniform (99% nucleotide identity) and form a distinct cluster, well separated from isolates occurring outside the EU (Juárez et al, 2019;Panno et al, 2019). Because these isolates are <94% identical to world ToLCNDV isolates, these EU isolates comprise a specific strain, ToLCNDV-ES (Fortes et al, 2016;Ruiz et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Pest survey card on tomato leaf curl New Delhi virus

Gemert¹,

Schenk²,

Candresse³

et al. 2020

EFS3

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This pest survey card was prepared in the context of the EFSA mandate on plant pest surveillance (M-2017-0137) at the request of the European Commission. Its purpose is to guide the Member States in preparing data and information for tomato leaf curl New Delhi virus (ToLCNDV) surveys. These are required to design statistically sound and risk-based pest surveys, in line with current international standards. Tomato leaf curl New Delhi virus is a clearly defined taxonomic entity. Although the main potential pathway of entry and spread of this virus via plants for planting of host plants is subject to prohibitions and specific measures designed to prevent entry and movement, the widespread occurrence and polyphagous nature of its vector Bemisia tabaci constitutes a risk for introduction and further spread of ToLCNDV given its persistent transmission biology. The virus is currently present in parts of the EU, and surveys in Member States can have several objectives, e.g. substantiation of disease freedom for countries or pest-free areas, or disease monitoring. The major hosts of ToLCNDV belong to the Solanaceae and Cucurbitaceae families, and detection surveys should thus target cultivated species belonging to these families. ToLCNDV is expected to be able to become established in most or all areas of the EU where B. tabaci is able to become established-outdoors or under protected cultivation-and where Solanaceae and Cucurbitaceae crops are grown. Surveillance should target symptomatic host plants in a fully grown crop. Because of the severity of its symptoms, surveillance of ToLCNDV can rely on visual examination. However, because its symptoms are similar or identical to those of other begomoviruses, its identification requires the use of validated laboratory assays.

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Emergence of tomato leaf curl New Delhi virus in Italy: estimation of incidence and genetic diversity

Cited by 37 publications

References 31 publications

Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques

Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques

Pest categorisation of tomato leaf curl New Delhi virus

Pest survey card on tomato leaf curl New Delhi virus

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