Emergence of plasmid-mediated tigecycline resistance tet(X4) gene in Escherichia coli isolated from poultry, food and the environment in South Asia

Mohsin, Mashkoor; Hassan, Brekhna; Martins, Willames M. B. S.; Li, Ruichao; Abdullah, Sabahat; Sands, Kirsty; Walsh, Timothy R.

doi:10.1016/j.scitotenv.2021.147613

Cited by 47 publications

(44 citation statements)

References 15 publications

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“…The emergence of mcr-1- and tet (X4)-cocarrying strains was worrisome, as the bacteria could be resistant to two last-resort antimicrobials, tigecycline and colistin. An increasing number of articles in the literature are reporting on the coexistence of mcr-1 and tet (X4) in one isolate but on two different plasmids ( 17 – 19 ). Xu et al reported the cocarriage of tet (X6) and mcr-1 by a single plasmid in Escherichia coli ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

Emerging Opportunity and Destiny of mcr-1 - and tet (X4)-Coharboring Plasmids in Escherichia coli

Xiao

et al. 2021

Microbiol Spectr

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Section: Introductionmentioning

confidence: 99%

Emerging Opportunity and Destiny of mcr-1 - and tet (X4)-Coharboring Plasmids in Escherichia coli

Xiao

et al. 2021

Microbiol Spectr

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“…A frameshift mutation (2-bp deletion) in the TetR/AcrR family transcriptional regulator was identified in strain C2051. Furthermore, strain C2051 was negative for the plasmid-mediated tigecycline resistance mechanism tet(X) , which encodes the tetracycline-inactivating enzyme, and the RND-type efflux pump gene cluster tmexCD1-toprJ1 , which was recently reported to confer resistance to tigecycline ( 18 , 19 ). Meanwhile, none of the novel mobile tigecycline resistance mechanisms were discovered, as tigecycline-nonsusceptible transconjugants were not acquired in the conjugation assays.…”

Section: Resultsmentioning

confidence: 99%

Emergence of Tigecycline Nonsusceptible and IMP-4 Carbapenemase-Producing K2-ST65 Hypervirulent Klebsiella pneumoniae in China

Zhang

Wang

et al. 2021

Microbiol Spectr

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“…Accordingly, the fitness cost elicited by resistance plasmids is closely associated with the dissemination of ARGs ( Alonso-Del Valle et al, 2021 ). Since its first description in China in 2019, tet (X4)-bearing plasmids have been found worldwide ( Ding et al, 2020 ; Zhang et al, 2020 ; Mohsin et al, 2021 ). For example, it is reported that tet (X4)-positive plasmids were widely distributed in the gut microbiota of Singaporeans ( Ding et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…For example, it is reported that tet (X4)-positive plasmids were widely distributed in the gut microbiota of Singaporeans ( Ding et al, 2020 ). Besides, tet (X4)-bearing plasmids were identified from poultry, food, and environmental samples in South Asia ( Mohsin et al, 2021 ). The prevalence of tet (X4) gene has posed a great challenge to human safety ( Fu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Histone-Like Nucleoid Structuring Protein Modulates the Fitness of tet(X4)-Bearing IncX1 Plasmids in Gram-Negative Bacteria

et al. 2021

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The emergence of plasmid-mediated tigecycline resistance gene tet(X4) poses a challenging threat to public health. Based on the analysis of tet(X4)-positive plasmids in the NCBI database, we found that the IncX1-type plasmid is one of the most common vectors for spreading tet(X4) gene, but the mechanisms by which these plasmids adapt to host bacteria and maintain the persistence of antibiotic resistance genes (ARGs) remain unclear. Herein, we investigated the underlying mechanisms of how host bacteria modulate the fitness cost of IncX1 plasmids carrying tet(X4) gene. Interestingly, we found that the tet(X4)-bearing IncX1 plasmids encoding H-NS protein imposed low or no fitness cost in Escherichia coli and Klebsiella pneumoniae; instead, they partially promoted the virulence and biofilm formation in host bacteria. Regression analysis revealed that the expression of hns gene in plasmids was positively linked to the relative fitness of host bacteria. Furthermore, when pCE2::hns was introduced, the fitness of tet(X4)-positive IncX1 plasmid pRF55-1 without hns gene was significantly improved, indicating that hns mediates the improvement of fitness. Finally, we showed that the expression of hns gene is negatively correlated with the expression of tet(X4) gene, suggesting that the regulatory effect of H-NS on adaptability may be attributed to its inhibitory effect on the expression of ARGs. Together, our findings suggest the important role of plasmid-encoded H-NS protein in modulating the fitness of tet(X4)-bearing IncX1 plasmids, which shed new insight into the dissemination of tet(X4) gene in a biological environment.

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Emergence of plasmid-mediated tigecycline resistance tet(X4) gene in Escherichia coli isolated from poultry, food and the environment in South Asia

Cited by 47 publications

References 15 publications

Emerging Opportunity and Destiny of mcr-1 - and tet (X4)-Coharboring Plasmids in Escherichia coli

Emerging Opportunity and Destiny of mcr-1 - and tet (X4)-Coharboring Plasmids in Escherichia coli

Emergence of Tigecycline Nonsusceptible and IMP-4 Carbapenemase-Producing K2-ST65 Hypervirulent Klebsiella pneumoniae in China

Histone-Like Nucleoid Structuring Protein Modulates the Fitness of tet(X4)-Bearing IncX1 Plasmids in Gram-Negative Bacteria

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