Emergence of plasmid-mediated quinolone resistance genes in clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa in Henan, China

Jiang, Xiaobing; Yu, Tao; Jiang, Xiaojie; Zhang, Wei; Zhang, Laiping; Ma, Jianmin

doi:10.1016/j.diagmicrobio.2014.03.025

Cited by 33 publications

(31 citation statements)

References 15 publications

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“…To the best of our knowledge, little data has been generated pertaining to the characterization of quinolone resistance mechanisms in LAB strains (Hummel et al 2007). In contrast, many similar studies have been performed in relation to other bacterial species such as Escherichia coli (Jiang et al 2014a), Salmonella (Kim et al 2011), Pseudomonas aeruginosa (Jiang et al 2014b), and Staphylococcus aureus (Ito et al 1994). In summary, the objective of this study was to investigate the characteristics associated with quinolone resistance mechanisms in yogurt-based LAB strains.…”

Section: Introductionmentioning

confidence: 82%

Characterization of quinolone resistance mechanisms in lactic acid bacteria isolated from yogurts in China

Jiang

Zhou

et al. 2016

Ann Microbiol

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Twenty-five strains of ciprofloxacin-resistant lactic acid bacteria (LAB; 14 Lactobacillus spp. and 11 Streptococcus thermophiles spp.) isolated from commercial yogurts in China were analyzed in this study. For each of these strains, amino acid changes associated with quinolone resistance-determining regions (QRDRs) were investigated using PCR-based detection methods. The same methodology was used to identify the presence of plasmid-mediated quinolone resistance (PMQR) genes in LAB. Sequencing analyses and an efflux pump inhibition test (using reserpine) were also performed as part of the analysis. Our results showed that typical mutations corresponding to quinolone resistance were found in the QRDRs of LAB strains. Detected mutations included Ser80Leu in parC, and Ser83Leu and Glu87Asp in gyrA. In addition, a Tyr74Phe substitution in parC, which had not previously been reported to be associated with quinolone resistance, was observed in two Lactobacillus delbrueckii subsp. bulgaricus strains. For each of the tested strains, the presence of the efflux pump inhibitor, reserpine, resulted in a two-to eightfold reduction in the minimum inhibitory concentrations (MICs) of ciprofloxacin. However, PMQR genes were not observed in any of the strains analyzed. Our results suggest that mutations in the QRDRs or efflux pump could be involved in ciprofloxacin resistance, and that a combination of these mechanisms may lead to increased ciprofloxacin resistance in LAB strains.

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Section: Introductionmentioning

confidence: 82%

Characterization of quinolone resistance mechanisms in lactic acid bacteria isolated from yogurts in China

Jiang

Zhou

et al. 2016

Ann Microbiol

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“…In these classes, members of the Campylobacteraceae, Aeromonadaceae, Enterobacteriaceae, Moraxellaceae, and Pseudomonadaceae were those with the highest decreases (data not shown). Given the fact that these groups comprise important human pathogens (Luangtongkum et al, 2010;Toleman et al, 2006;Miró et al, 2010;Jiang et al, 2014), this variation meets one of the key requirements of wastewater treatmentthe removal of pathogens. The balanced increase of some bacterial groups and a decrease of others resulted in the higher diversity and equitability indices after the secondary treatment (Table S3), an effect that was not reverted after disinfection as has been reported by other authors (Hu et al, 2012;Ye and Zhang, 2013).…”

Section: Discussionmentioning

confidence: 99%

Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant

Narciso-da-Rocha

Rocha

Vaz‐Moreira

et al. 2018

Environment International

109

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Urban wastewater treatment plants (UWTPs) are reservoirs of antibiotic resistance. Wastewater treatment changes the bacterial community and inevitably impacts the fate of antibiotic resistant bacteria and antibiotic resistance genes (ARGs). Some bacterial groups are major carriers of ARGs and hence, their elimination during wastewater treatment may contribute to increasing resistance removal efficiency. This study, conducted at a full-scale UWTP, evaluated variations in the bacterial community and ARGs loads and explored possible associations among them. With that aim, the bacterial community composition (16S rRNA gene Illumina sequencing) and ARGs abundance (real-time PCR) were characterized in samples of raw wastewater (RWW), secondary effluent (sTWW), after UV disinfection (tTWW), and after a period of 3 days storage to monitoring possible bacterial regrowth (tTWW-RE). Culturable enterobacteria were also enumerated. Secondary treatment was associated with the most dramatic bacterial community variations and coincided with reductions of ~2 log-units in the ARGs abundance. In contrast, no significant changes in the bacterial community composition and ARGs abundance were observed after UV disinfection of sTWW. Nevertheless, after UV treatment, viability losses were indicated ~2 log-units reductions of culturable enterobacteria. The analysed ARGs (qnrS, bla, bla, bla, bla, sul1, sul2, and intI1) were strongly correlated with taxa more abundant in RWW than in the other types of water, and which associated with humans and animals, such as members of the families Campylobacteraceae, Comamonadaceae, Aeromonadaceae, Moraxellaceae, and Bacteroidaceae. Further knowledge of the dynamics of the bacterial community during wastewater treatment and its relationship with ARGs variations may contribute with information useful for wastewater treatment optimization, aiming at a more effective resistance control.

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“…Otherwise qnrD has a higher frequency detection (91.43 %). These results implied the wider distribution of qnrS and qnrD than oqxB, which may be explained by the fact that qnrS and qnrD were plasmid-mediated quinolone resistance (PMQR) genes (Jiang et al 2014a), while oqxB was generally located in genome DNA.…”

Section: Quinolone Resistance Genesmentioning

confidence: 96%

Prevalence of antibiotic resistance genes of wastewater and surface water in livestock farms of Jiangsu Province, China

Chen

Lü

Guo

et al. 2015

Environ Sci Pollut Res

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The overuse of antibiotics in livestock farms is general, leading to a wide distribution of antibiotic resistance genes (ARGs) in aquatic environment adjacent to livestock farms. However, researches of the distribution and types of ARGs in aquatic environment of China are still in the initial stage. In this study, wastewater and surface water samples were collected from 12 livestock farms (four pig farms, four cattle farms, and four chicken farms) in Jiangsu Province of China. The prevalence, abundance, and distribution of 22 ARGs were investigated, which were categorized into six groups, including nine tetracyclin resistance genes, three sulfonamides resistance genes, three quinolone resistance genes, two macrolide resistance genes, three aminoglycoside resistance genes, and two multidrug resistance genes, employing quantitative real-time PCR (qPCR). The results suggested that all of the 22 ARGs were detected in samples. Sul1, sul2, and tetM were the most abundant with the average concentration of 3.84×10 1 copies/16S recombinant RNA (rRNA) gene copies, 1.62 × 10 1 copies/16S rRNA gene copies, 2.33 × 10 1 copies/16S rRNA gene copies, respectively. Principle component analysis revealed that the comprehensive pollution of ARGs in northern Jiangsu was more serious. ARGs in wastewater were more abundant when compared to that in surface water. A preliminary study regarding the fate of ARGs after an aerobiotic process showed that tetA, tetC, sul1, sul2, oqxB, and qnrS were significantly increased. And, among the tetracycline resistance genes, the efflux pump genes were enriched while the ribosomal protection protein encoding genes were decreased in the aerobiotic process. The prevalance of ARGs in water environment is of concern; more surveillance is required to determine the pollution level and pattern of antibiotic resistance genes.

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Emergence of plasmid-mediated quinolone resistance genes in clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa in Henan, China

Cited by 33 publications

References 15 publications

Characterization of quinolone resistance mechanisms in lactic acid bacteria isolated from yogurts in China

Characterization of quinolone resistance mechanisms in lactic acid bacteria isolated from yogurts in China

Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant

Prevalence of antibiotic resistance genes of wastewater and surface water in livestock farms of Jiangsu Province, China

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