Emergence of OXA-48 carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in dogs

Stolle, Inka; Prenger‐Berninghoff, Ellen; Stamm, Ivonne; Scheufen, Sandra; Hassdenteufel, Esther; Guenther, Sebastian; Bethe, Astrid; Pfeifer, Yvonne; Ewers, Christa

doi:10.1093/jac/dkt259

Cited by 159 publications

(115 citation statements)

References 38 publications

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“…Hence, almost 20% of the owners considered here (including family members) could be at risk of acquiring these genes from their dogs. Moreover, in contrast to a recent report in diseased dogs in Germany (23), no carbapenemase producer was found. However, ChromID ESBL plates may have been impaired in detecting certain phenotypes, such as OXA-48 producers in the absence of ESBL coexpression.…”

Section: Resultscontrasting

confidence: 99%

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Haenni¹,

Saras²,

Métayer³

et al. 2014

Antimicrob Agents Chemother

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bIn the community, close contacts between humans and dogs may promote the transfer of extended-spectrum beta-lactamase/ plasmidic AmpC cephalosporinase (ESBL/pAmpC) genes. Large-scale prevalence studies on ESBL/pAmpC carriage in dogs are rare, and data on ESBL/pAmpC plasmids are even more limited. Here, a considerable rate of 18.5% ESBL/pAmpC carriers was found among 368 unrelated healthy dogs in Paris, France. This prevalence is much higher than the one found in healthy humans in the same city (6%) but close to that recently reported in dogs in China (24.5%). All isolates were identified as Escherichia coli, except one Salmonella enterica and one Klebsiella pneumoniae isolate. The sequence type 131 (ST131) clone was rare (2/73 isolates). Interestingly, two plasmids (bla CTX-M-1 /IncI1/ST3 and bla CMY-2 /IncI1/ST2) were unexpectedly highly predominant, raising the question of their successful spread. Considering that CTX-M-1 was recently found to be equally as abundant as CTX-M-15 in healthy Parisian subjects, the question of dogs being a CTX-M-1 reservoir for humans is open. Such a high prevalence of the bla CMY-2 /IncI1/ST2 plasmid may result from the use of cephalexin in veterinary medicine, as previously demonstrated experimentally. In all, our study points out healthy urban dogs as a potential source of ESBL/pAmpC genes that can further disseminate to the human community.

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Section: Resultscontrasting

confidence: 99%

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Haenni¹,

Saras²,

Métayer³

et al. 2014

Antimicrob Agents Chemother

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“…The same study identified, ESBL producing S. enterica, Proteus miriabilis and Enterobacter cloaceae from urinary, wound, respiratory, abdominal and bone infections (Dierikx et al, 2012). In Germany from six nosocomially infected dogs, OXA-48 type Carbopenamases producing E. coli and K. pneumonia were isolated (Stolle et al, 2013) and in France CTX-M-15 generating K. pneumoniae have been detected from urinary tract infections of dogs and cats (Haenni et al, 2012). Clinical samples from urinary, wound infections etc.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 86%

Nosocomial Infections and their Surveillance in Veterinary Hospitals

Milton¹

2015

Adv. Anim. Vet. Sci.

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| Nosocomial, or hospital-acquired, infections are well-thought-out to be the most common problem affecting hospitalized human patients as well as veterinary patients. These are of great consequence in veterinary medicine and can have considerable adverse effects on the individual patient as well as on the veterinary hospital as a whole. Different infectious agents like MRSA, Cl. difficile, MDR E. coli, Salmonella spp. and many more are of prodigious significance in causing NIs. A surveillance and control measure for these NIs in veterinary settings needs to be addressed in future. This review consolidates some Nosocomial infections and the strategy for its surveillance in veterinary practice.

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“…During the past few years, cases of infections caused by K. pneumoniae strains resistant to clinically important classes of antibiotics, including third-generation cephalosporins (3GCs), have also been reported in companion animals (2)(3)(4)(5)(6). 3GCs represent important antibiotics for the treatment of serious infections caused by K. pneumoniae before the use of last-resort carbapenems.…”

mentioning

confidence: 99%

Third-Generation-Cephalosporin-Resistant Klebsiella pneumoniae Isolates from Humans and Companion Animals in Switzerland: Spread of a DHA-Producing Sequence Type 11 Clone in a Veterinary Setting

Wohlwend

Endimiani

Francey

et al. 2015

Antimicrob Agents Chemother

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Characterization of third-generation-cephalosporin-resistant Klebsiella pneumoniae isolates originating mainly from one human hospital (n ‫؍‬ 22) and one companion animal hospital (n ‫؍‬ 25) in Bern (Switzerland) revealed the absence of epidemiological links between human and animal isolates. Human infections were not associated with the spread of any specific clone, while the majority of animal infections were due to K. pneumoniae sequence type 11 isolates producing plasmidic DHA AmpC. This clonal dissemination within the veterinary hospital emphasizes the need for effective infection control practices. The rapid spread of multidrug-resistant (MDR) Klebsiella pneumoniae has led to major concerns in hospitals (1). During the past few years, cases of infections caused by K. pneumoniae strains resistant to clinically important classes of antibiotics, including third-generation cephalosporins (3GCs), have also been reported in companion animals (2-6). 3GCs represent important antibiotics for the treatment of serious infections caused by K. pneumoniae before the use of last-resort carbapenems. Transmission of 3GC-resistant K. pneumoniae (3GC-R-Kp) between companion animals and humans represents a possible threat to both human and animal health (7). This prompted us to determine which resistance determinants and clonal lineages are associated with 3GC-R-Kp obtained primarily from one human hospital as well as from one companion animal clinic in Bern, Switzerland.Isolates were selected based on decreased susceptibility to cefotaxime or ceftazidime (MICs of both, Ն1 g/ml) (8). Species identification was confirmed by using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (Bruker Daltonik) (9). The human isolates consisted of all 3GC-R-Kp isolates from patients admitted to different wards of the same hospital (hospital 1 [H1]) between 2013 and 2014 (n ϭ 21), except one (5208.51) which came from H2. K. pneumoniae isolates were predominantly recovered from urine and less frequently from blood and biopsy specimens (Table 1). Multilocus sequence typing (MLST) (http://www.pasteur.fr/recherche /genopole/PF8/mlst/) and XbaI pulsed-field gel electrophoresis (PFGE) profiles (contour-clamped homogeneous electric field [CHEF] DR-III apparatus [Bio-Rad]; run time, 18.5 h; gradient, 6 V/cm; initial switch time, 2.2 s; final switch time, 54.2 s; angle, 120°) (10) revealed that the human isolates differed genetically from each other; each patient was infected with a unique strain that exhibited a distinct PFGE profile, and all of the isolates belonged to different sequence types (ST) except two isolates that were of ST101 and three that were of ST873 (Fig. 1). The absence of clonal spread of K. pneumoniae, even within the same ward, is suggestive of infection control best practices at the hospital. Such diversity indicates that resistance may emerge independently of the wards in different K. pneumoniae lineages, e.g., through the acquisition of resistance to 3GCs. In contrast, only two different ...

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Emergence of OXA-48 carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in dogs

Abstract: This is the first known report of OXA-48-producing bacteria from companion animals. The clonal nature of the K. pneumoniae and two E. coli isolates suggests a nosocomial dissemination rather than repeated introduction by individual patients into the clinic.

Cited by 159 publications

References 38 publications

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Nosocomial Infections and their Surveillance in Veterinary Hospitals

Third-Generation-Cephalosporin-Resistant Klebsiella pneumoniae Isolates from Humans and Companion Animals in Switzerland: Spread of a DHA-Producing Sequence Type 11 Clone in a Veterinary Setting

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Emergence of OXA-48 carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in dogs

Abstract: This is the first known report of OXA-48-producing bacteria from companion animals. The clonal nature of the K. pneumoniae and two E. coli isolates suggests a nosocomial dissemination rather than repeated introduction by individual patients into the clinic.

Cited by 159 publications

References 38 publications

High Prevalence of bla CTX-M-1 /IncI1/ST3 and bla CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

High Prevalence of bla CTX-M-1 /IncI1/ST3 and bla CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Nosocomial Infections and their Surveillance in Veterinary Hospitals

Third-Generation-Cephalosporin-Resistant Klebsiella pneumoniae Isolates from Humans and Companion Animals in Switzerland: Spread of a DHA-Producing Sequence Type 11 Clone in a Veterinary Setting

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High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France