Emergence of Omicron BA.2.12.1 in Pakistan: What does it mean for the South Asian nation?

Awan, Hashir Ali; Awan, Sana; Shaikh, Hamid Saeed; Swed, Sarya; Hasan, Mohammad Mehedi

doi:10.1016/j.amsu.2022.103943

Cited by 2 publications

(1 citation statement)

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“…The NovaType III SARS-CoV-2 assay became available in August 2021 and was used until December 2021 for the identification of key lineage mutations for; Delta/Epsilon (E484, L452R), Kappa (E484Q, L452R) and Beta/Gamma (E484K) variants. By December 2021, the Omicron variant (BA.1) was identified [ 9 ], with its characteristic deletion in Spike codon 69–70 leading to a S- gene Target Failure (SGTF) identified on the TaqPath™ COVID‑19 CE‑IVD RT‑PCR assay [ 10 ]. Thus, we employed the TaqPath assay until February 2022, keeping the NovaType III assay for screening of additional specimens.…”

Section: Resultsmentioning

confidence: 99%

Tracking SARS-CoV-2 variants through pandemic waves using RT-PCR testing in low-resource settings

Nasir

Aamir

Kanji

et al. 2023

PLOS Glob Public Health

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COVID-19 resulted in extensive morbidity and mortality worldwide. SARS-CoV-2 evolved rapidly, with increasing transmission due to Variants of Concern (VOC). Identifying VOC became important but genome submissions from low-middle income countries (LMIC) remained low leading to gaps in genomic epidemiology. We demonstrate the use of a specific mutation RT-PCR based approach to identify VOC in SARS-CoV-2 positive samples through the pandemic in Pakistan. We selected 2150 SARS-CoV-2 PCR positive respiratory specimens tested between April 2021 and February 2022, at the Aga Khan University Hospital Clinical Laboratories, Karachi, Pakistan. Commercially available RT-PCR assays were used as required for mutations in Spike protein (N501Y, A570D, E484K, K417N, L452R, P681R and deletion69_70) to identify Alpha, Beta, Gamma, Delta, and Omicron variants respectively. Three pandemic waves associated with Alpha, Delta and Omicron occurred during the study period. Of the samples screened, VOC were identified in 81.7% of cases comprising mainly; Delta (37.2%), Alpha (29.8%) and Omicron (17.1%) variants. During 2021, Alpha variants were predominant in April and May; Beta and Gamma variants emerged in May and peaked in June; the Delta variant peaked in July and remained predominant until November. Omicron (BA.1) emerged in December 2021 and remained predominant until February 2022. The CT values of Alpha, Beta, Gamma and Delta were all significantly higher than that of Omicron variants (p<0.0001). We observed VOC through the pandemic waves using spike mutation specific RT-PCR assays. We show the spike mutation specific RT-PCR assay is a rapid, low-cost and adaptable for the identification of VOC as an adjunct approach to NGS to effectively inform the public health response. Further, by associating the VOC with CT values of its diagnostic PCR we gain information regarding the viral load of samples and therefore the level of transmission and disease severity in the population.

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Section: Resultsmentioning

confidence: 99%