Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe

Cointe, Aurélie; Bizot, Etienne; Delannoy, Sabine; Fach, Patrick; Bidet, Philippe; Birgy, André; Weill, François-Xavier; Lefèvre, Sophie; Mariani‐Kurkdjian, Patricia; Bonacorsi, Stéphane

doi:10.3390/toxins13100686

Cited by 5 publications

(7 citation statements)

References 26 publications

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“…Several non-O157 AE-STEC serogroups are important causes of human disease, one of the most common being O80:H2, for which hypotheses of stx genes transduction were already discussed in the literature 39 , 40 . This study demonstrates that stx2d -harboring phages from AE-STEC O80:H2 can transduce EPEC O80:H26 and to non-pathogenic strains, and convert them to stable lysogens unlike the O80:H2 strain.…”

Section: Discussionmentioning

confidence: 99%

Impact of Shiga-toxin encoding gene transduction from O80:H2 Shiga toxigenic Escherichia coli (STEC) on non-STEC strains

Habets

Antoine

Wagemans

et al. 2022

Sci Rep

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Shiga toxin-producing Escherichia coli (STEC) are major foodborne pathogens that cause human diseases ranging from diarrhea to life-threatening complications including hemolytic–uremic syndrome. Virulence of STEC strains and their ability to cause severe diseases are associated with the activity of prophage-encoded Shiga toxins (Stxs). The first objective of this work was to isolate and characterize the Stx2d phage from STEC O80:H2 and to study the transfer of this phage in non-STEC strains. The second objective was to assess the survival of Galleria mellonella larvae inoculated with these transduced strains. Firstly, one bacteriophage isolated from a STEC O80:H2 strain was used to infect six non-STEC strains, resulting in the conversion of three strains. Then, stability assays were performed, showing that this phage was stable in the new STEC strains after three successive subculturing steps, as confirmed by a combination of short and long read genome sequencing approaches. This phage, vB_EcoS_ULI-O80_Stx2d, is resistant to moderate temperature and pH. It belongs to a currently unclassified genus and family within the Caudoviricetes class, shares 98% identity with Stx2_112808 phage and encodes several proteins involved in the lysogenic cycle. The yecE gene was identified at the insertion site. Finally, G. mellonella experiments showed that the transduced strains caused significantly higher mortality rates than the corresponding non-STEC strains. In conclusion, this study showed that stx2d gene from O80:H2 E. coli can be transferred to non-STEC strains and contributes to their virulence.

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Section: Discussionmentioning

confidence: 99%

Impact of Shiga-toxin encoding gene transduction from O80:H2 Shiga toxigenic Escherichia coli (STEC) on non-STEC strains

Habets

Antoine

Wagemans

et al. 2022

Sci Rep

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“…The virulence genes largely varied depending on the identified clones ( Supplementary Table S2 ), but ST301 has particularly caught our attention. This clone, which was found in four isolates of breeding farm #52, was an O80:H2 enterohemorrhagic EHEC possessing, among others, the stx2 , eae and ehxA virulence genes ( Cointe et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…This indicates on the one hand that these isolates may be co-selected by the use of different classes of antibiotics, and on the other hand that they may become pathogenic either for the animals or for humans if the context allows. In particular, one adult goat and three kids in breeding farm #52 presented an O80:H2 enterohemorrhagic EHEC possessing the stx2 , eae and ehxA virulence genes ( Cointe et al, 2021 ). This serotype was among the three major ones causing pediatric haemolytic uraemic syndrome (HUS) in 2015 in France ( Bruyand et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Spread of the mcr-1 colistin-resistance gene in Escherichia coli through plasmid transmission and chromosomal transposition in French goats

et al. 2023

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IntroductionColistin-resistance widely disseminated in food-producing animals due to decades of colistin use to treat diarrhea. The plasmid-borne mcr-1 gene has been extensively reported from bovine, swine and chicken worldwide, but smaller productions such as the goat farming sector were much less surveyed.MethodsWe looked for colistin-resistant isolates presenting plasmid-borne genes of the mcr family in both breeding (n=80) and fattening farms (n=5). Localization of the mcr-1 gene was performed using Southern blot analysis coupled to short-read and long-read sequencing.ResultsOnly the mcr-1 gene was identified in 10% (8/80) of the breeding farms and four over the five fattening farms. In total, 4.2% (65/1561) of the animals tested in breeding farms and 60.0% (84/140) of those tested in fattening farms presented a mcr-1-positive E. coli. The mcr-1 gene was located either on the chromosome (32.2%) or on IncX4 (38.9%) and IncHI2 (26.8%) plasmids. As expected, both clonal expansion and plasmidic transfers were observed in farms where the mcr-1 gene was carried by plasmids. Tn6330 transposition was observed in the chromosome of diverse E. coli sequence types within the same farm.DiscussionOur results show that the mcr-1 gene is circulating in goat production and is located either on plasmids or on the chromosome. Evidence of Tn6330 transposition highlighted the fact that chromosomal insertion does not impair the transmission capability of the mcr-1 gene. Only strict hygiene and biosecurity procedures in breeding farms, as well as a prudent use of antibiotics in fattening farms, can avoid such complex contamination pathways.

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“…Serotype O80:H2 represents one of these “minor” serotypes that has been emerging since 2010 in France and other Western European countries in humans suffering (bloody) diarrhea, hemolytic–uremic syndrome, and bacteremia [ 13 , 14 , 15 , 16 , 17 , 18 ]. These AE-STEC O80:H2 combine not only the pathogenic properties of EPEC and STEC, but also of SePEC since they can invade the blood stream and colonize internal organs.…”

Section: Introductionmentioning

confidence: 99%

“…These AE-STEC O80:H2 combine not only the pathogenic properties of EPEC and STEC, but also of SePEC since they can invade the blood stream and colonize internal organs. The invasiveness properties are encoded by genes located on a pS88-like ColV conjugative plasmid [ 15 , 16 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Virulence of Shigatoxigenic and Enteropathogenic Escherichia coli O80:H2 in Galleria mellonella Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d Phage

Ikeda,

Laforêt,

Antoine

et al. 2023

Veterinary Sciences

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The invasiveness properties of Shigatoxigenic and enteropathogenic Escherichia coli (STEC and EPEC) O80:H2 in humans and calves are encoded by genes located on a pS88-like ColV conjugative plasmid. The main objectives of this study in larvae of the Galleria mellonella moth were therefore to compare the virulence of eight bovine STEC and EPEC O80:H2, of two E. coli pS88 plasmid transconjugant and STX2d phage transductant K12 DH10B, of four E. coli O80:non-H2, and of the laboratory E. coli K12 DH10B strains. Thirty larvae per strain were inoculated in the last proleg with 10 μL of tenfold dilutions of each bacterial culture corresponding to 10 to 106 colony-forming units (CFUs). The larvae were kept at 37 °C and their mortality rate was followed daily for four days. The main results were that: (i) not only the STEC and EPEC O80:H2, but also different E. coli O80:non-H2 were lethal for the larvae at high concentrations (from 104 to 106 CFU) with some variation according to the strain; (ii) the Stx2d toxin and partially the pS88 plasmid were responsible for the lethality caused by the E. coli O80:H2; (iii) the virulence factors of E. coli O80:non-H2 were not identified. The general conclusions are that, although the Galleria mellonella larvae represent a useful first-line model to study the virulence of bacterial pathogens, they are more limited in identifying their actual virulence properties.

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Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe

Cited by 5 publications

References 26 publications

Impact of Shiga-toxin encoding gene transduction from O80:H2 Shiga toxigenic Escherichia coli (STEC) on non-STEC strains

Impact of Shiga-toxin encoding gene transduction from O80:H2 Shiga toxigenic Escherichia coli (STEC) on non-STEC strains

Spread of the mcr-1 colistin-resistance gene in Escherichia coli through plasmid transmission and chromosomal transposition in French goats

Virulence of Shigatoxigenic and Enteropathogenic Escherichia coli O80:H2 in Galleria mellonella Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d Phage

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