Emergence of NDM-5-Producing Escherichia coli in a Teaching Hospital in Chongqing, China: IncF-Type Plasmids May Contribute to the Prevalence of blaNDM–5

Zhang, Hua; Jia, Xiaojiong; Liu, Huan; Li, Shuang; Wu, Xiuquan; Huang, Shifeng

doi:10.3389/fmicb.2020.00334

Cited by 30 publications

(23 citation statements)

References 26 publications

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“…1a ). A similar structure has been found on an IncFIA/FIB plasmid (pNDM-5-1001) of an E. coli ST410 clinical isolate from China ( 12 ) and on an IncFII plasmid (pNDM-5-IT) of an E. coli ST167 clinical isolate from Italy ( 13 ), although there is a difference in the direction of the mphA - mrx - mphR module. Two copies of bla OXA-48 , both located between two copies of insA - insB (IS 1R ), were present on the chromosome of FUJ80155 ( Fig.…”

Section: Observationsupporting

confidence: 64%

Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure

et al. 2021

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Although patients with history of international hospitalization are often subject to screening for multidrug-resistant organisms, it is unclear whether patients who reside in countries where carbapenemase-producing Enterobacterales (CPE) is endemic but have no history of local hospitalization contribute to the transmission of CPE. In this study, NDM-5-producing and OXA-48-producing Escherichia coli sequence type (ST) 648, a recently recognized high-risk, multidrug-resistant clone, were detected from two overseas visitors without previous medical exposure.

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Section: Observationsupporting

confidence: 64%

Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure

et al. 2021

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“…IncF type plasmids have also been shown to contribute to the dissemination of bla NDM-5 among E. coli [17,18,46]. In this study, we observed that the bla NDM-5 in E. coli from neonates was carried on IncFIA type plasmids.…”

Section: Discussionsupporting

confidence: 49%

“…The bla NDM-5 from these isolates co-existed with bla CTX-M-15 , bla TEM-1B and bla OXA-1 located on the IncFIA plasmid. Other recent studies in Africa and elsewhere have observed co-harbouring of bla NDM-5 and bla CTX-15 , bla TEM-1B , bla OXA-1 in the same plasmid type in E. coli [3,12,46]. Co-localization of bla NDM-5 and extended spectrum β-lactamase (bla CTX-M-15 ) plus other resistance genes in the same plasmid could increase the dissemination of multiple resistances in a single gene transfer event.…”

Section: Discussionmentioning

confidence: 91%

First identification of bla NDM-5 producing Escherichia coli from neonates and a HIV infected adult in Tanzania

Manyahi

Moyo

Kibwana

et al. 2022

Journal of Medical Microbiology

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Introduction. Carbapenem-resistant members of the family Enterobacteriaceae are emerging as a global public-health threat and cause substantial challenges in clinical practice. Gap Statement. There is a need for increased and continued genomic surveillance of antimicrobial resistance genes globally in order to detect outbreaks and dissemination of clinically important resistance genes and their associated mobile genetic elements in human pathogens. Aim. To describe the resistance mechanisms of carbapenem-resistant Escherichia coli . Methods. Rectal swabs from neonates and newly diagnosed human immunodeficiency virus (HIV) infected adults were collected between April 2017 and May 2018 and screened for faecal carriage of carbapenamases and OXA-48 producing members of the family Enterobacteriaceae. Bacterial isolates were identified using matrix assisted laser desorption ionization time of flight mass spectrometry. Antimicrobial susceptibility testing was performed by E-test. Whole genomes of carbapenem-resistant E. coli were investigated using a hybrid assembly of Illumina and Oxford Nanopore Technologies sequencing reads. Results. Three carbapenem-resistant E. coli were detected, two from neonates and one from an HIV infected adult. All three isolates carried bla NDM-5. Two E. coli from neonates belonged to ST167 and bla NDM-5 co-existed with bla CTX-M-15 and bla OXA-01, and all were carried on IncFIA type plasmids. The E. coli from the HIV infected adult belonged to ST2083, and carried bla NDM-5 on an IncX3 type plasmid and bla CMY-42 on an IncI type plasmid. All bla NDM-5 carrying plasmids contained conjugation related genes. In addition, E. coli from the HIV infected adult carried three more plasmid types; IncFIA, IncFIB and Col(BS512). One E. coli from a neonate also carried one extra plasmid Col(BS512). All three E. coli harboured resistance genes to fluoroquinolone, aminoglycosides, sulfamethoxazole, trimethoprim, macrolides and tetracycline, carried on the IncFIA type plasmid. Furthermore, E. coli from the neonates carried a chloramphenicol resistance gene (catB3), also on the IncFIA plasmid. All three isolates were susceptible to colistin. Conclusion. This is the first report, to our knowledge, from Tanzania detecting bla NDM-5 producing E. coli. The carbapenemase gene was carried on an IncFIA and IncX3 type plasmids. Our findings highlight the urgent need for a robust antimicrobial resistance (AMR) surveillance system to monitor and rapidly report on the incidence and spread of emerging resistant bacteria in Tanzania.

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“…Our results found that AR_N1_I is highly homologous to bla NDM– 1 surrounding fragments encoded by Enterobacter cloacae174 chromosome, indicating that bla NDM– 1 surrounding fragments could transfer between the IncX3 plasmid and Enterobacter chromosome. In 2020, Zou et al (2020) reported that the genetic environment containing bla NDM– 5 could be divided into five subtypes. Among them, Type A corresponds to the AR_N5_I genetic environment in our study.…”

Section: Discussionmentioning

confidence: 99%

In silico Evolution and Comparative Genomic Analysis of IncX3 Plasmids Isolated From China Over Ten Years

et al. 2021

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IncX3 plasmids are correlated with the dissemination and acquisition of carbapenem resistance in Enterobacteriaceae and have been prevalent in China over the last 10 years. Since the distribution characteristics of IncX3 plasmids across China as well as their evolutionary traits for 10 years remain unclear, here we conducted a retrospective literature review and in silico comparative analysis of IncX3 plasmids in publicly available IncX3 plasmid genomes. IncX3 plasmids distributed in 17 provinces or cities were extracted for analysis, which tend to be specifically associated with hospital-isolated Escherichia coli ST410 from phylogroup A. Although the backbones of IncX3 plasmids have remained highly conservative over the last 10 years, the blaNDM resistance genetic contexts on these plasmids could fall into five subtypes, among which AR_N1_I has been identified in Enterobacter cloacae174 chromosome and AR_N5_I was simultaneously located on IncF and IncA/C plasmids. This suggests that the blaNDM resistance gene environment can spread between different plasmids, between different bacterial genera, or between strains and plasmids, highlighting that it is imperative to adopt more stringent infection control measures targeting IncX3 plasmid spread.

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Emergence of NDM-5-Producing Escherichia coli in a Teaching Hospital in Chongqing, China: IncF-Type Plasmids May Contribute to the Prevalence of blaNDM–5

Cited by 30 publications

References 26 publications

Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure

Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure

First identification of bla NDM-5 producing Escherichia coli from neonates and a HIV infected adult in Tanzania

In silico Evolution and Comparative Genomic Analysis of IncX3 Plasmids Isolated From China Over Ten Years

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