Emergence of multidrug-resistant Acinetobacter baumannii producing OXA-23 Carbapenemase in Qatar

Abstract

The objective of our study was to describe the molecular support of carbapenem resistance from randomly selected clinical isolates of multidrug-resistant (MDR) Acinetobacter baumannii as a pilot study from the Hamad Medical Corporation (HMC), Qatar. Results of our report will be used to study carbapenemases using molecular techniques in all isolated MDR A. baumannii. Forty-eight MDR A. baumannii were randomly selected from isolates preserved at HMC. Identification of all isolates was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antibiotic resistance was tested phenotypically by Phoenix and confirmed by Etest. The molecular support of carbapenemases (blaOXA-23, blaOXA-24, blaOXA-58, blaNDM) was investigated by real-time PCR. The epidemiologic relatedness of the isolates was verified by phylogenetic analysis based on partial sequences of CsuE and blaOXA-51 genes. All 48 isolates were identified as A. baumannii and were confirmed to be resistant to most antibiotics, especially meropenem, imipenems, ciprofloxacin, levofloxacin, amikacin, gentamicin and most of the β-lactams; they were sensitive to colistin. All the isolates were positive for blaOXA-23 and negative for the other tested carbapenemase genes. Clonality analysis demonstrated that different lineages were actually circulating in Qatar; and we suggest that an outbreak occurred in the medical intensive care unit of HMC between 2011 and 2012. Here we report the emergence of MDR A. baumannii producing the carbapenemase OXA-23 in Qatar.

Characterization of Carbapenem Resistant Acinetobacter baumannii causing Ventilator associated Pneumonia in ICUs of Zagazig University Hospitals, Egypt

“…The percentage of bla OXA-23 -like gene detected in impenem resistant isolates were 69.7% (23/33), this result is in agreement with Zowawi et al, 2015;Luo et al, 2015, different percentages of presence of bla OXA-et al, 2013), 100% by Fouad et al 2013, and others also reported all over the world as those reported by Cherkaoue et al, 2015(51.8%), Val et al, 2015(85%), 85.7% ElAbd et al, 2014and 100% Rolain et al, 2016 None of the impenem resistant isolates were positive for bla OXA-24 -like or bla OXA-58 -like genes this result was in agreement with Rolain et al, (2016). However bla OXA-58 -like, and bla OXA-24/40 -like genes were detected in lower rate than ofbla OXA-23 -like genes in many studies in Egypt as that by Al Agamy et al, (2014)who reported that A. baumannii carryingbla OXA-58 -like gene were 5% and that carrying bla OXA-24/40 -like gene is 7.5% ,and another study by AlHassan et al(2013) reported that the percentage of bla , and bla OXA-58 were 2.9%, and 14.7%, respectively.…”

Effect of Antibiotic Combinations on the Sensitivity of Carbapenem Resistant Acinetobacter baumannii Strains

“…Also, our results were nearly confirmed by Gao et al [28] who confirmed that (100%) of the carbapenem resistant isolates showed positive results for the presence of OXA 51 gene, (94%) for OXA 23, and the other two resistance genes (OXA 24 and OXA 58) were not detected in any of the strains. Same results were revealed by Rolain et al [29] in Hamad hospital in Qatar, that conveyed that (100%) of A. baumannii isolates were positive for OXA-23; none were positive for OXA-24, OXA-58 [30].…”

Retrospective Analysis on Antimicrobial Resistance Trends and Prevalence of β-lactamases in Escherichia coli and ESKAPE Pathogens Isolated from Arabian Patients during 2000–2020

“…Those studies showed that during the study period, ESBL prevalence ranged between 9% to 31.7% based on the data with clinical isolates ( n = 2166) [ 44 , 46 , 47 ]. Molecular characterization with the use of PCR demonstrated that bla OXA and bla CTX-M were the most prevalent ESBL traits among E. coli and ESKAPE pathogens, a smaller number of clinical isolates were reported to express bla TEM and, bla SHV ESBL traits ( Table 2 ) [ 44 , 46 , 47 , 48 ].…”