Emergence of Methicillin-Resistant Staphylococcus aureus ST239 with High-Level Mupirocin and Inducible Clindamycin Resistance in a Tertiary Care Center in Chennai, South India

Abimanyu, Nagarajan; Saravanan, Muthupandian; Krishnan, Padma

doi:10.1128/jcm.01663-12

Cited by 31 publications

(22 citation statements)

References 9 publications

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“…Our previous study from Iran, carried out to identify molecular characteristics of MRSA isolated from blood samples, showed that ST239 was the most predominant type (43%). In line with the studies conducted by Abimanyu et al in India [22] and Udo et al in Kuwait [20], who demonstrated that HLMUPR-MRSA strains belonged to ST239 clone, our findings showed that 12 HLMUPR-MRSA strains (14.3%) and 5 HLMUPR-MRSA strains (5.9%) belonged to ST239. Among our ST239 isolates, 12 (14.3%), 3 (3.6%), and 2 isolates (2.4%) were positive for tst, eta and etb genes, respectively.…”

Section: Discussionsupporting

confidence: 93%

Molecular investigation of methicillin-resistant staphylococcus aureus isolates from blood: USA600 emerges as the major type

Goudarzi

Sabzehali

Heidary

et al. 2018

J Infect Dev Ctries

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Introduction: The widespread emergence of methicillin-resistant Staphylococcus aureus is turning into a real worry in public health. The goals of the present study were to identify resistance and virulence encoding genes and molecular characteristics of methicillin-resistant S. aureus bloodstream isolates. Methodology: A cross-sectional study was conducted on 84 S. aureus bloodstream isolates during a 10-month period. To evaluate antibiotic susceptibility of the isolates, we used Kirby-Bauer disk diffusion method. In addition, the prevalence of antimicrobial resistance and toxins genes was assessed using polymerase chain reaction. Isolates were typed according to polymorphisms seven housekeeping genes by MLST. Results: All the isolates were resistant to methicillin. The most prevalent resistance gene was mecA gene (100%) followed by tetM (57.1%), aac (6΄)-Ie/aph (2˝) (53.6%), ant (4΄)-Ia (46.4%), ermA (45.2%), msrA (35.7%), msrB (33.3%), aph (3΄)-IIIa (33.3%), ermB (31%), ermC (16.7%), and mupA (14.3%) genes. The presence of toxin encoding genes tst, pvl, eta, and etb were detected in 25%, 14.3%, 3.6% and 3.6%, respectively. The isolates were classified into five different sequence types: ST45 (29.8%), ST239 (27.4%), ST858 (21.4%), ST22 (17.8%), and ST59 (3.6%). All the high-level mupirocin-resistant (HLMUPR) strains belonged to ST239, while the low-level mupirocin resistant (LLMUPR) strains belonged to ST22 (13%) and ST239 (6%). Conclusions: To the best of our knowledge, the present study is the first report of ST59 in MRSA bloodstream isolates in Iran. Our data demonstrated the need for thorough epidemiological monitoring to detect emergence and dissemination of MDR- MRSA types in our hospitals.

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Section: Discussionsupporting

confidence: 93%

Molecular investigation of methicillin-resistant staphylococcus aureus isolates from blood: USA600 emerges as the major type

Goudarzi

Sabzehali

Heidary

et al. 2018

J Infect Dev Ctries

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“…This agrees with a previous study showing SCC mec ‐III MRSAs with spa type t037 as the most predominant group circulating in a single hospital over 5‐month surveillance periods in Malaysia . Globally, there were other reports on the presence of MRSA with spa type t037 from clinical samples in India and USA . The second most common spa type among our MRSAs was spa type t8696, which is the least reported isolate globally.…”

Section: Discussionsupporting

confidence: 92%

Nasal colonisation, antimicrobial susceptibility and genotypic pattern of Staphylococcus aureus among agricultural biotechnology students in Besut, Terengganu, east coast of Malaysia

Suhaili

Yeo

Faizal

et al. 2018

Tropical Med Int Health

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“…In a study conducted in 2017 in Iran by Goudarzi et al (10), it was found that HLMUPR-MRSA strains belonged to ST15-SCCmec IV/t084 (40%), ST22-SCCmec IV/t790 (23.3%), ST239-SCCmec III/t631 (20%), and ST239-SCCmec III/t030 (16.7%) clones. In this study, it was shown that all the ST239-SCCmec III/t631 strains harbored the mupA gene, which is consistent with the studies of Abimanyu et al (34) and Boswihi et al (29) who reported high-level mupirocin resistance in the MRSA ST239 clone. Similar to the data from the previous studies, we found that none of the isolates contained class 3 integron (12).…”

Section: Discussionsupporting

confidence: 91%

Molecular Analysis and Integron Carriage of Mupirocin-Resistant Staphylococcus aureus Strains Isolated from Burn Wound Infections, Tehran, Iran

Navidinia

Goudarzi

Pouriran

et al. 2019

Arch Clin Infect Dis

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Background:The prevalence of wound infections associated with multidrug-resistant Staphylococcus aureus (S. aureus) strains among burns patients has tremendously increased. Recently, the role of integrons as a key system involved in spreading multi-drug resistance has been well documented. Objectives: To investigate the prevalence of integrons, antimicrobial resistance pattern, and S. aureus protein A (spa) typing of the mupirocin-resistant S. aureus (MRSA) strains obtained from a referral burns hospital in Tehran, Iran. on 64 MRSA isolates gathered from burns patients. The isolates were examined for susceptibility to antimicrobial agents. The integrase-encoding gene was detected by conventional polymerase chain reaction (PCR). Integrase-positive strains were evaluated for the determination of integron classes by using PCR-restriction fragment length polymorphism assay, and genotyping was performed by spa typing. Results: Out of 64 MRSA strains, 24 (37.5%) and 40 (62.5%) isolates carried high-level and low-level resistance, respectively. The findings revealed that the entire isolates were MRSA. They belonged to six different spa types including t860 (40.6%), t790 (21.8%), t037 (17.2%), t064 (17.2%), t008 (1.6%), and t631 (1.6%). Class 1 and 2 integrons were found in 59.4% and 17.2% of the isolates, respectively, and 4 (6.2%) isolates were observed to carry class 1 and 2 integrons simultaneously. Conclusions: Precise epidemiological monitoring of integrons is required to prevent mupirocin resistance dissemination in MRSA strains.

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Emergence of Methicillin-Resistant Staphylococcus aureus ST239 with High-Level Mupirocin and Inducible Clindamycin Resistance in a Tertiary Care Center in Chennai, South India

Cited by 31 publications

References 9 publications

Molecular investigation of methicillin-resistant staphylococcus aureus isolates from blood: USA600 emerges as the major type

Molecular investigation of methicillin-resistant staphylococcus aureus isolates from blood: USA600 emerges as the major type

Nasal colonisation, antimicrobial susceptibility and genotypic pattern of Staphylococcus aureus among agricultural biotechnology students in Besut, Terengganu, east coast of Malaysia

Molecular Analysis and Integron Carriage of Mupirocin-Resistant Staphylococcus aureus Strains Isolated from Burn Wound Infections, Tehran, Iran

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