Emergence of mcr-1- and blaNDM-5-harbouring IncHI2 plasmids in Escherichia coli strains isolated from meat in Zhejiang, China

Wang, Jingge; Tang, Biao; Lin, Rumeng; Zheng, Xue Bin; Ma, Jian-Gang; Xiong, Xiaoxia; Jiang, Han; Yang, Hua; Ding, Baoan

doi:10.1016/j.jgar.2022.06.002

Cited by 9 publications

(11 citation statements)

References 6 publications

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“…Isolate 12 harbors a P0111_1 plasmid, isolate 19 harbors a IncI2_1 plasmid, and isolate 189 harbors a IncI2_1_Delta plasmid. There have been studies showing that mcr-1 harboring IncI plasmids were prevalent in various Enterobacterales in China, including animal-originated E. coli isolates (Tang et al, 2022;Wang et al, 2022), Escherichia fergusonii, and Salmonella (Tang et al, 2020;Tang et al, 2023), which were also observed in our study.…”

Section: Phylogenetic Analysissupporting

confidence: 84%

Prevalence and molecular characteristics of polymyxin-resistant Enterobacterales in a Chinese tertiary teaching hospital

Xiao

Li²,

Huang³

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionPolymyxin-resistant Enterobacterales poses a significant threat to public health globally, but its prevalence and genomic diversity within a sole hospital is less well known. In this study, the prevalence of polymyxin-resistant Enterobacterales in a Chinese teaching hospital was investigated with deciphering of their genetic determinants of drug resistance.MethodsPolymyxin-resistant Enterobacterales isolates identified by matrix-assisted laser desorption were collected in Ruijin Hospital from May to December in 2021. Both the VITEK 2 Compact and broth dilution methods were used to determine polymyxin B (PMB) susceptibility. Polymyxin-resistant isolates were further characterized by molecular typing using PCR, multi-locus sequence typing, and sequencing of the whole genome.ResultsOf the 1,216 isolates collected, 32 (2.6%) across 12 wards were polymyxin-resistant (minimum inhibitory concentration (MIC) range, PMB 4–256 mg/ml, and colistin 4 ≥ 16 mg/ ml). A total of 28 (87.5%) of the polymyxin-resistant isolates had reduced susceptibility to imipenem and meropenem (MIC ≥ 16 mg/ml). Of the 32 patients, 15 patients received PMB treatment and 20 survived before discharge. The phylogenetic tree of these isolates showed they belonged to different clones and had multiple origins. The polymyxin-resistant Klebsiella pneumoniae isolates belonged to ST-11 (85.72%), ST-15 (10.71%), and ST-65 (3.57%), and the polymyxin-resistant Escherichia coli belonged to four different sequence types, namely, ST-69 (25.00%), ST-38 (25.00%), ST-648 (25.00%), and ST-1193 (25.00%). In addition, six mgrB specific mutations (snp_ALT c.323T>C and amino acid change p.Val8Ala) were identified in 15.6% (5/32) of the isolates. mcr-1, a plasmid-mediated polymyxin-resistant gene, was found in three isolates, and non-synonymous mutations including T157P, A246T, G53V, and I44L were also observed.DiscussionIn our study, a low prevalence of polymyxin-resistant Enterobacterales was observed, but these isolates were also identified as multidrug resistant. Therefore, efficient infection control measures should be implemented to prevent the further spread of resistance to last-line polymyxin therapy.

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Section: Phylogenetic Analysissupporting

confidence: 84%

Prevalence and molecular characteristics of polymyxin-resistant Enterobacterales in a Chinese tertiary teaching hospital

Xiao

Li²,

Huang³

et al. 2023

Front. Cell. Infect. Microbiol.

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“…At the same time, the AMR rates of A/C (3.31%), MEM (2.76%), CL (5.52%) and CAZ (7.18%) were low. CL and MEM are recognized as the last line of defense against gram-negative bacteria ( Lin et al, 2022 ; Wang et al, 2022 ) and low AMR rates reveal their resistance’s effectiveness is decreasing.…”

Section: Discussionmentioning

confidence: 99%

“…The recent emergence and rapid increase of multi-drug resistant (MDR, resistance to more than three kinds of antibiotics) bacteria have caused public concern, represented by Escherichia coli resistant to carbapenem, colistin and tigecycline which were recognized as the last line of resort ( Li et al, 2017 ; He et al, 2019 ; Ma et al, 2022 ). Due to incorrect use and misuse of antibiotics, the spread of antimicrobial resistance (AMR) is accelerating ( Wang et al, 2022 ; Tang et al, 2022a , b ). The continuous spread of AMR not only increases the difficulty of preventing and controlling livestock and poultry diseases but also seriously threatens livestock products’ safety and endangers consumers’ health ( Xu et al, 2022 ; Li et al, 2022a ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

et al. 2022

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Escherichia coli is considered an opportunistic pathogen and an indicator for antimicrobial resistance (AMR) monitoring. Despite many reports on its AMR monitoring, studies based on genome-based analysis of AMR genes are still insufficient. Here, 181 E. coli strains were isolated from anal swab samples collected from pigs and chickens of animal farms located in Eastern China and sequenced through the Illumina platform. The results showed that 87.85% (159/181) of the E. coli isolates were multidrug-resistant (MDR). Ampicillin (AMP)- spectinomycin (SPT)- tetracycline (TET)- florfenicol (FFC)- sulfisoxazole (SF)- trimethoprim/sulfamethoxazole (SXT) was the predominant AMR pattern. By whole-genome sequencing, we found that ST10 (10.49%, 19/181) and ST48 (7.18%, 13/181) were major sequence types. IncFIB and IncX1 were the most prevalent plasmid replicons. The AMR genes blaNDM-5 (1.10%, 2/181), mcr-1 (1.10%, 2/181), tet(X4) (1.10%, 2/181), and cfr (6.08%, 2/181) were also found in these isolates. In addition, among the 169 virulence genes detected, we identified astA (37.02%, 67/181), hlyA (1.66%, 3/181), hlyB (1.66%, 3/181) and hlyD (1.66%, 3/181), which were closely related to heat-stable enterotoxin 1 and α-hemolysin. In addition, there were 33 virulence genes associated with the iron uptake system, and 46 were adhesion-related genes. Our study highlighted the need for routine surveillance of AMR with advanced genomic approaches, providing up-to-date data on the prevalence of AMR for the development and execution of antimicrobial stewardship policy.

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“…Meanwhile, the backbone structure of bla NDM-5 -carrying the IncX3-type plasmid was highly conserved, including the ISAb125-IS5-bla NDM-5 -bleO-trpF-tat-cutA-IS26 gene cassette, the T4SS systems, the plasmid replication/maintenance genes and mobile elements IS3000, Tn2 (Figure 5). Moreover, bla NDM-5 gene was mostly located on the Incx3-type plasmid in MNPECs, regardless of the location of the mcr-1 gene on the chromosome or plasmid (Yu et al, 2016;Kong et al, 2017;Ho et al, 2018;Kuang et al, 2022;Wang et al, 2022). This indicated that IncX3-type plasmid was the dominant vector for carrying bla NDM-5 and disseminate the carbapenem resistance in MNPECs.…”

Section: Discussionmentioning

confidence: 97%

Genetic characteristic of coexisting of mcr-1 and blaNDM-5 in Escherichia coli isolates from lesion-bearing animal organs

Xiang

Liu

et al. 2023

Front. Microbiol.

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The coexistence of mcr-1 and blaNDM-5 in the plasmid of Escherichia coli has been widely reported and such strains have been mainly isolated from animal and human feces. However, few reports have focused on the genetic diversity of mcr-1-carrying chromosomes and blaNDM-5-carrying plasmids in E. coli isolates from lesion-bearing animal organs. This study investigated the genetic characteristics of chromosome-mediated mcr-1 and plasmid-mediated blaNDM-5 in E. coli isolated from lesion-bearing animal organs. Nine mcr-1- and blaNDM-5-positive E. coli strains (MNPECs) showed extensive drug resistance (XDR). The predominant clonal complexes (CC) mainly belonged to CC156, CC10, and CC165 from the 56 MNEPCs (including nine strains in this study) retrieved from the literature. These strains were widely distributed in China, and originated from pig fecal samples, human stool/urine samples as well as intestinal contents of chicken. Two transconjugants harboring blaNDM-5 gene were also successfully obtained from two donors (J-8 and N-14) and this transfer increased the MIC for meropenem by 256 times. However, conjugative transfer of mcr-1 gene failed. Both J-8 and N-14 strains contained point mutations associated with quinolone resistance and more than three types of AMR genes, including the mcr-1 gene on the chromosome and the blaNDM-5 gene on the IncX3-type plasmid. The genetic structure of mcr-1 located on the chromosome was an intact Tn6330, and blaNDM-5-carrying IncX3-type plasmid was ISAb125-IS5-blaNDM-5-bleO-trpF-tat-cutA-IS26 gene cassette. Moreover, differences between chromosomes included additional partial sequence of phage integrated into host genome and the different genes associated with O-antigen synthesis.

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Emergence of mcr-1- and blaNDM-5-harbouring IncHI2 plasmids in Escherichia coli strains isolated from meat in Zhejiang, China

Cited by 9 publications

References 6 publications

Prevalence and molecular characteristics of polymyxin-resistant Enterobacterales in a Chinese tertiary teaching hospital

Prevalence and molecular characteristics of polymyxin-resistant Enterobacterales in a Chinese tertiary teaching hospital

Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

Genetic characteristic of coexisting of mcr-1 and blaNDM-5 in Escherichia coli isolates from lesion-bearing animal organs

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