Emergence of genetically unrelated NDM-1-producing Acinetobacter pittii strains in Paraguay

Pasterán, Fernando; Mora, Mario Martínez; Albornoz, Ezequiel; Faccone, Diego; Franco, Rossana; Ortellado, Juana; Melgarejo, Nancy; Gómez, Sonia; Riquelme, Irma; Matheu, Jorge; Ramón-Pardo, Pilar; Corso, Alejandra

doi:10.1093/jac/dku139

Cited by 29 publications

(32 citation statements)

References 13 publications

(11 reference statements)

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“…NDM-1 producers generally associated with Enterobacteriaceae species have also been reported, albeit with much lower frequency, in Latin American countries, including Guatemala, Mexico, Colombia, and Brazil (3). Moreover, production of NDM-1 in this geographic region has also been noted in Acinetobacter baumannii in Honduras and Brazil (3,4) and in Acinetobacter pittii in Paraguay and Brazil (5,6). Here, we report the first case of an NDM-1-producing Acinetobacter species in Argentina, an Acinetobacter bereziniae clinical isolate.…”

supporting

confidence: 51%

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Brovedan

Marchiaro

Morán-Barrio

et al. 2015

Antimicrob Agents Chemother

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b The New Delhi metallo-␤-lactamase (NDM-1) was initially identified in clinical isolates of Escherichia coli and Klebsiella pneumoniae in Sweden from a patient previously hospitalized in India (1). Since then, bla NDM-1 has frequently been reported in Enterobacteriaceae and Acinetobacter spp., with a fast dissemination in the Indian subcontinent, the Balkan countries, China, and the Middle East (2). NDM-1 producers generally associated with Enterobacteriaceae species have also been reported, albeit with much lower frequency, in Latin American countries, including Guatemala, Mexico, Colombia, and Brazil (3). Moreover, production of NDM-1 in this geographic region has also been noted in Acinetobacter baumannii in Honduras and Brazil (3,4) and in Acinetobacter pittii in Paraguay and Brazil (5, 6). Here, we report the first case of an NDM-1-producing Acinetobacter species in Argentina, an Acinetobacter bereziniae clinical isolate. We describe also the complete sequence of a bla NDM-1 -containing plasmid in this strain.(Part of this work was presented at the 10th International Symposium on the Biology of Acinetobacter 2015, Athens, Greece, 3 to 6 June 2015 [7].)A. bereziniae HPC229 was isolated on June 2014 from a blood sample of a 53-year-old female patient that underwent chemotherapy due to leukemia at a hospital located in Rosario, Argentina. The patient was treated with ciprofloxacin plus tigecycline, resulting in clinical and microbiological cure as evaluated by negative blood cultures. The patient was readmitted to the hospital 3 months later with symptoms of severe sepsis and died due to septic shock, with positive blood cultures that grew Escherichia coli.HPC229, originally identified as Acinetobacter lwoffii by the Vitek 2 system (bioMérieux), was reclassified by DNA sequence comparison analyses of its 16S rRNA, gyrB (8), and rpoB (9) genes. The highest identity was found to the corresponding orthologs of the A. bereziniae ATCC 17924 type strain (99.9%, 99.7%, and 99.8%, respectively). The antibiotic susceptibility profile of HPC229 was determined by either the Vitek 2 system or the agar dilution method. The interpretation of the obtained MICs based on CLSI breakpoints (10) indicated resistance to ␤-lactams, including carbapenems (Table 1).PCR amplification using specific primers for the bla IMP , bla VIM , bla SPM , bla NDM , bla OXA-23-like , bla OXA-24/40-like , and bla OXA-58-like genes (11-13) and sequencing analysis revealed the presence of bla NDM-1 . Evidence that bla NDM-1 was located in a plasmid was first obtained by plasmid curing (14) in which HPC229 was cultured in 5 ml of LB liquid medium containing 0.2 ml of 10% SDS for 48 h at 40°C. This procedure allowed the isolation of HPC229c, which showed a marked increase in susceptibility to ␤-lactams (Table 1). Susceptibility to aztreonam as judged by the disk diffusion assay, in contrast, showed no differences between HPC229 and HPC229c. All conjugation attempts using HPC229 as the donor and Escherichia coli DH5␣, A. baumannii ATCC 17978, or Pseudo...

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supporting

confidence: 51%

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Brovedan

Marchiaro

Morán-Barrio

et al. 2015

Antimicrob Agents Chemother

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“…Other reports from China, Egypt, and France followed (5)(6)(7)27). The Latin American countries Honduras (28) and Paraguay (29) have also reported Acinetobacter pittii isolates. ST25 NDM-1-positive A. baumannii originating from the Balkan region has been reported in European studies (13,25).…”

mentioning

confidence: 93%

“…Although NDM-1-positive Enterobacteriaceae have been isolated in Rio Grande do Sul (10,11), approximately 1,200 km south of Londrina, there was no history of patient transfer between these locations. Recently, NDM-positive A. pittii was isolated in Rio Grande do Sul (A. Barth, personal communication) and in the bordering country of Paraguay (29), but there is no apparent epidemiological link. Further investigations are under way to establish a hypothesis for the origin of this bla NDM-1 plasmid.…”

mentioning

confidence: 99%

First Report of NDM-1-Producing Acinetobacter baumannii Sequence Type 25 in Brazil

Pillonetto

Arend²,

Vespero

et al. 2014

Antimicrob Agents Chemother

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New Delhi metallo-β-lactamase 1 (NDM-1) was first identified in Brazil in Enterobacter hormaechei and Providencia rettgeri in 2013. Here, we describe the first case of NDM-1-producing Acinetobacter baumannii sequence type 25 isolated from the urinary tract of a 71-year-old man who died of multiple complications, including A. baumannii infection. The NDM-1 gene was detected by quantitative PCR, and its sequence confirmed its presence in an ∼ 100-kb plasmid.

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“…Multilocus sequence typing (MLST) was performed according to the Institute Pasteur scheme (http://www.pasteur.fr) and A. pittii isolate MP was identified as ST119. Interestingly, two bla NDM -positive A. pittii isolates were recently identified in Paraguay [4], a neighbouring country of Brazil, but those isolates belonged to ST320 and ST321. The only reports of A. pittii ST119 isolates are from Japan, with isolates producing the carbapenemase IMP-19 [1].…”

mentioning

confidence: 99%

Emergence of NDM-1-producing Acinetobacter pittii in Brazil

Pagano¹,

Poirel²,

Martins³

et al. 2015

International Journal of Antimicrobial Agents

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Emergence of genetically unrelated NDM-1-producing Acinetobacter pittii strains in Paraguay

Cited by 29 publications

References 13 publications

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

First Report of NDM-1-Producing Acinetobacter baumannii Sequence Type 25 in Brazil

Emergence of NDM-1-producing Acinetobacter pittii in Brazil

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Emergence of genetically unrelated NDM-1-producing Acinetobacter pittii strains in Paraguay

Cited by 29 publications

References 13 publications

Complete Sequence of a bla NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Complete Sequence of a bla NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

First Report of NDM-1-Producing Acinetobacter baumannii Sequence Type 25 in Brazil

Emergence of NDM-1-producing Acinetobacter pittii in Brazil

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Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina