Emergence of Escherichia coli harbouring mcr-1 and mcr-3 genes in North West Algerian farmlands

Touati, Meriem; Hadjadj, Linda; Berrazeg, Meryem; Baron, Sophie Alexandra; Rolain, Jean‐Marc

doi:10.1016/j.jgar.2019.10.001

Cited by 52 publications

(33 citation statements)

References 19 publications

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“…Resistance genes to antimicrobials and metals are indicated in red and blue, respectively, whereas virulence genes are indicated in orange. (Touati et al, 2020), respectively. In Brazil, mcr-type genes from soil samples have only been detected in total DNA or cultivable microbiota so far (Oliveira et al, 2019;Furlan et al, 2020b;Dos Santos et al, 2020).…”

Section: Discussionmentioning

confidence: 97%

Colistin-Resistant mcr-1-Positive Escherichia coli ST131-H22 Carrying blaCTX–M–15 and qnrB19 in Agricultural Soil

et al. 2021

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The pandemic Escherichia coli sequence type 131 (ST131) carrying plasmid-mediated colistin resistance mcr genes has emerged worldwide causing extraintestinal infections, with lineages belonging to three major clades (A, B, and C). Clade B is the most prevalent in animals, contaminating associated meat products, and can be transmitted zoonotically. However, the blaCTX–M–15 gene has only been associated with C2 subclade so far. In this study, we performed a genomic investigation of an E. coli (strain S802) isolated from a kale crop in Brazil, which exhibited a multidrug-resistant (MDR) profile to clinically significant antimicrobials (i.e., polymyxin, broad-spectrum cephalosporins, aminoglycosides, and fluoroquinolones). Whole-genome sequencing analysis revealed that the S802 strain belonged to serotype O25:H4, ST131/CC131, phylogenetic group B2, and virotype D5. Furthermore, S802 carried the clade B-associated fimH22 allele, genes encoding resistance to clinically important antimicrobials, metals, and biocides, and was phylogenetically related to human, avian, and swine ST131-H22 strains. Additionally, IncHI2-IncQ1, IncF [F2:A-:B1], and ColE1-like plasmids were identified harboring mcr-1.1, blaCTX–M–15, and qnrB19, respectively. The emergence of the E. coli ST131-H22 sublineage carrying mcr-1.1, blaCTX–M–15, and qnrB19 in agricultural soil represents a threat to food and environmental safety. Therefore, a One Health approach to genomic surveillance studies is required to effectively detect and limit the spread of antimicrobial-resistant bacteria and their resistance genes.

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Section: Discussionmentioning

confidence: 97%

Colistin-Resistant mcr-1-Positive Escherichia coli ST131-H22 Carrying blaCTX–M–15 and qnrB19 in Agricultural Soil

et al. 2021

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“…As mentioned above, the use of wastewater and manure for the irrigation and fertilization of soil may be the main uses responsible for the vast dissemination of the mcr-1 gene. Recently, two Escherichia coli harboring mcr-1 were isolated from horse (n = 1) and bovine (n = 1) manure, and the plasmid carrying mcr-1 isolated from the horse manure was successfully transferred by conjugation [116]. The sul1 and sul2 genes from pig slurry and manured agricultural soil samples were transferred from a variety of Gram-negative bacterial species to Escherichia coli K-12 CV601 and Pseudomonas putida UWC1 recipient cells by conjugation at different rates, indicating their presence on different mobile elements [64].…”

Section: Args Spread By Hgt Mechanismsmentioning

confidence: 99%

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Lima

Domingues

Silva

2020

Veterinary Sciences

125

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The increasing demand for animal-derived foods has led to intensive and large-scale livestock production with the consequent formation of large amounts of manure. Livestock manure is widely used in agricultural practices as soil fertilizer worldwide. However, several antibiotic residues, antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria are frequently detected in manure and manure-amended soils. This review explores the role of manure in the persistence and dissemination of ARGs in the environment, analyzes the procedures used to decrease antimicrobial resistance in manure and the potential impact of manure application in public health. We highlight that manure shows unique features as a hotspot for antimicrobial gene dissemination by horizontal transfer events: richness in nutrients, a high abundance and diversity of bacteria populations and antibiotic residues that may exert a selective pressure on bacteria and trigger gene mobilization; reduction methodologies are able to reduce the concentrations of some, but not all, antimicrobials and microorganisms. Conjugation events are often seen in the manure environment, even after composting. Antibiotic resistance is considered a growing threat to human, animal and environmental health. Therefore, it is crucial to reduce the amount of antimicrobials and the load of antimicrobial resistant bacteria that end up in soil.

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“…Since the co-occurrence of two mcr genes in a single isolate has been reported, [7][8][9] we tested the ability of our multiplex PCR method to simultaneously detect mixed DNA templates in a single tube. As shown in Figure 2, the multiplex PCR system can effectively detect 28 combinations of two genes and 56 combinations of three genes.…”

Section: Validation Of the Multiplex Pcr Methodsmentioning

confidence: 99%

“…6 Most plasmid-mediated colistin-resistant strains carry a single mcr gene; however, co-presence of two mcr genes in a single isolate has been described recently. [7][8][9] MCR family proteins are membrane-associated phosphoethanolamine (PEA) transferases which catalyze the addition of PEA moiety to the lipopolysaccharides (LPS)-lipid A, resulted in reduced affinity to colistin. 10 To prevent the spread of colistin resistance, fast, cheap and efficient diagnostic approaches are desperately needed.…”

Section: Introductionmentioning

confidence: 99%

<p>Molecular Detection of the <em>mcr</em> Genes by Multiplex PCR</p>

Liu

Zhang

Feng

et al. 2020

IDR

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Background: The emergence and prevalence of plasmid-mediated colistin-resistant bacterial strains in recent years have raised great concerns in clinical medicine. It is urgently needed to develop a cheaper, faster, simpler, sensitive, and specific molecular detection method to identify and monitor the dissemination of the transferable resistant determinants. Methods and Results: Herein, eight pairs of primers were designed to set up a multiplex PCR method for the rapid and efficient determination of reported mcr genes. This assay can give results within 85 min (35 min for amplification and 50 min for electrophoresis). We validated the feasibility of this assay by testing the presence of mcr genes in 60 colistinresistant isolates. Conclusion: Our multiplex PCR technique exhibits remarkable advantages in the light of clear identification, efficiency of amplification, as well as the time consuming for detection, and thus could be useful for the surveillance and epidemiological research of plasmidmediated colistin resistance, particularly for the under-resourced laboratories.

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Emergence of Escherichia coli harbouring mcr-1 and mcr-3 genes in North West Algerian farmlands

Cited by 52 publications

References 19 publications

Colistin-Resistant mcr-1-Positive Escherichia coli ST131-H22 Carrying blaCTX–M–15 and qnrB19 in Agricultural Soil

Colistin-Resistant mcr-1-Positive Escherichia coli ST131-H22 Carrying blaCTX–M–15 and qnrB19 in Agricultural Soil

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

<p>Molecular Detection of the <em>mcr</em> Genes by Multiplex PCR</p>

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