Emergence of Enteric Viruses in Production Chickens Is a Concern for Avian Health

Mettifogo, Elena; Núñez, L; Chacón, Jorge Luis; Parra, Silvana Hipatia Santander; Astolfi-Ferreira, Claudete S.; Jerez, José Antonio; Jones, Richard C.; Ferreira, Antônio José Piantino

doi:10.1155/2014/450423

Cited by 59 publications

(84 citation statements)

References 45 publications

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“…In a subsequent study, however, these authors (Pantin-Jackwood et al, 2008) reported ARVs in 62.80% of the chicken flocks and 45.50% of the turkey flocks tested. Previous studies have reported the presence of ARV in intestinal/faecal samples of broiler chickens (Koo et al, 2013;Mettifogo et al, 2014) although most of these studies indicated low prevalence of ARV as compared to other enteric viruses.…”

Section: Discussionmentioning

confidence: 99%

“…Several pathogens such as avian rotavirus, astrovirus, reovirus, coronavirus, adenovirus, Salmonella, Escherichia coli, Enterococcus, and Eimeria spp. have been detected either alone or in different combinations from broiler chickens and turkey flocks afflicted with enteritis (Barnes et al, 2000;Jindal et al, 2010;Pantin-Jackwood et al, 2007, 2008Day and Zsak, 2013;Koo et al, 2013;Mettifogo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Serological studies suggested widespread prevalence of ANV infections in commercial broiler chickens in various countries (Imada et al, 1980;Connor et al, 1987). In recent years, ANVs have been detected in enteritis-or RSS-affected broiler chickens from many different countries by molecular methods (Pantin-Jackwood et al, 2008;Smyth et al, 2009;Roussan et al, 2012;Koo et al, 2013;Mettifogo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The detection/isolation of CAstVs has been reported from broiler chickens exhibiting RSS and from dead-in-shell chicks having hatchability problems (Baxendale and Mebatsion, 2004;Smyth et al, 2009;Pantin-Jackwood et al, 2011;Kang et al, 2012;Koo et al, 2013;Smyth et al, 2013;Mettifogo et al, 2014;SajewiczKrukowska et al, 2016). In most of these studies, CAstVs have been detected using reverse transcription PCR (RT-PCR) and/or quantitative RT-PCR targeting ORF1b or ORF2 genes.…”

Section: Introductionmentioning

confidence: 99%

“…The ARVs have been detected in enteritis-or RSS-affected broiler chickens (Davis et al, 2013;Koo et al, 2013;Mettifogo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Detection and molecular characterization of enteric viruses in enteritis-affected commercial broiler chickens in India

Kaithal¹,

Jindal²,

Kumar³

et al. 2016

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Summary. -A study was conducted to detect and characterize the enteric viruses (chicken astrovirus, avian nephritis virus and avian orthoreovirus) present in flocks of commercial broiler chickens suffering from enteritis in Haryana, India. The intestinal contents were collected from 65 enteritis-affected flocks (cases) and tested by reverse transcription PCR (RT-PCR). Of these 65 cases, 35 (53.80%) were positive for a single virus and 26 (40.00%) for two viruses. The remaining four samples were negative for all three viruses tested. Of the 65 cases, 57 were positive for chicken astrovirus (CAstV) while 30 cases had avian nephritis virus (ANV). None of the cases were positive for orthoreovirus. Comparison of 12 CAstVs of this study with previously published CAstV sequences revealed nucleotide identities ranging from 73.20 to 98.00%. The nucleotide identities ranged between 83.10-95.50% when nine ANVs of this study were compared with previously reported ANV sequences. The amino acid sequences of CAstVs in comparison to previously published sequences revealed certain unique changes. Phylogeny based on polymerase gene revealed that CAstVs and ANVs of this study were under the same monophyletic clade. In conclusion, a large number of broiler chicken flocks experiencing enteritis were positive for CAstV and ANV by RT-PCR. The presence of more than one enteric virus in enteritis-affected flocks and changes at the genetic level in these viruses may affect the severity of disease.Keywords: chicken astrovirus; avian nephritis virus; avian orthoreovirus; broiler chicken; India * Corresponding author. E-mail: nareshjindal1@gmail.com; phone: +91-1662-289323. Abbreviations: ANV = avian nephritis virus; ARV = avian reovirus; CAstV = chicken astrovirus; GI = gastrointestinal; PES = poult enteritis syndrome; RSS = runting and stunting syndrome; RT-PCR = reverse transcription PCR

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The ARVs have been detected in enteritis-or RSS-affected broiler chickens (Davis et al, 2013;Koo et al, 2013;Mettifogo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection and molecular characterization of enteric viruses in enteritis-affected commercial broiler chickens in India

Kaithal¹,

Jindal²,

Kumar³

et al. 2016

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Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil

et al. 2016

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The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens of broiler chickens in Brazil. During 2008 and 2011, a total of 85 fecal samples were collected. The viral genome was extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequencing. Samples were screened for rotaviruses by PAGE, and RVF and RVG genome banding patterns were not seen. Using RT-PCR, it was found that 9.4 % (8/85) of the pools contained RVF, whereas 10.6 % (9/85) contained RVG. The predicted amino acid sequences of RVF and RVG from Brazilian samples were 94.4-95.7 % and 96.8-96.9 % identical, respectively, to those of prototypes from Germany. The detection of RVF and RVG in this study provides important epidemiological data about the simultaneous circulation of rotaviruses affecting broiler flocks in the Amazon region of Brazil.

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Avian nephritis virus (ANV) on Brazilian chickens farms: circulating genotypes and intra-genotypic diversity

Beserra

Soares

et al. 2016

Arch Virol

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Avian nephritis virus (ANV), which belongs to the family Astroviridae, is associated with different clinical manifestations (including enteric disorders). Despite being frequently found in the avian industry worldwide, information regarding genetic features of these viruses in Brazil is scarce. Therefore, sixty fecal sample pools (5-6 birds of the same flock), representing 60 poultry farms from six Brazilian States, were screened using an astrovirus-specific hemi-nested-PCR assay targeting the conserved ORF1b gene, followed by nucleotide sequencing of amplified products. PCR and phylogenetic analysis confirmed the detection of 21 positive samples to ANV (35 %). In order to investigate the genetic diversity represented by these viruses, amplification, cloning and phylogenetic analysis of the deduced amino acid sequence of ORF2 gene were attempted. Eight samples were successfully cloned (generating 32 clones in total) and sequenced. Based on phylogenetic analysis of ORF2, sequences defined in this study were classified into three genotypes: genotype 5, which has already been described in birds, and two other novel genotypes, tentatively named genotype 8 and 9, all of which occurred in single or mixed infections. Moreover, high intra-genotypic diversity and co-circulation of distinct strains in a same host population were observed. This study revealed the presence of new strains of ANV in Brazilian poultry and their circulation in commercial chicken flocks.

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Emergence of Enteric Viruses in Production Chickens Is a Concern for Avian Health

Cited by 59 publications

References 45 publications

Detection and molecular characterization of enteric viruses in enteritis-affected commercial broiler chickens in India

Detection and molecular characterization of enteric viruses in enteritis-affected commercial broiler chickens in India

Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil

Avian nephritis virus (ANV) on Brazilian chickens farms: circulating genotypes and intra-genotypic diversity

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