Emergence of Community-Associated Methicillin-Resistant Staphylococcus aureus at a Memphis, Tennessee Children??s Hospital

Buckingham, Steven C.; McDougal, Linda K.; Cathey, Lorene D.; Comeaux, Katha; Fridkin, Scott K.; Tenover, Fred C.

doi:10.1097/01.inf.0000131981.67342.c4

Cited by 168 publications

(128 citation statements)

References 35 publications

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“…An isolate of the same lineage as USA400 (also known as S. aureus MW-2) was responsible for a series of infections in health care settings across Canada (61), infections in Native Americans (31) and among children in day care (33), and an outbreak of S. aureus infection on a maternity ward of a hospital in New York (62). USA300 isolates have been recovered from a variety of community populations, including children (5,39), correctional facility inmates (7,10), participants in sports teams (9,40), men who have sex with men (8,34,42), and military recruits (72). Over the last 3 years, outbreak investigations conducted by the Centers for Disease Control and Prevention (CDC) in diverse geographic locations and with diverse patient populations often yielded the same USA300 PFGE pattern, designated USA300-0114, from wound cultures and other clinical specimens (40).…”

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confidence: 99%

Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States

Tenover¹,

McDougal²,

Goering³

et al. 2006

Journal of Infection

151

221

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A highly stable strain of Staphylococcus aureus with a pulsed-field gel electrophoresis type of USA300 and multilocus sequence type 8 has been isolated from patients residing in diverse geographic regions of the United States. This strain, designated USA300-0114, is a major cause of skin and soft tissue infections among persons in community settings, including day care centers and correctional facilities, and among sports teams, Native Americans, men who have sex with men, and military recruits. The organism is typically resistant to penicillin, oxacillin, and erythromycin (the latter mediated by msrA) and carries SCCmec type IVa. This strain is variably resistant to tetracycline [mediated by tet(K)]; several recent isolates have decreased susceptibility to fluoroquinolones. S. aureus USA300-0114 harbors the genes encoding the Panton-Valentine leucocidin toxin. DNA sequence analysis of the direct repeat units within the mec determinant of 30 USA300-0114 isolates revealed differences in only a single isolate. Plasmid analysis identified a common 30-kb plasmid that hybridized with blaZ and msrA probes and a 3.1-kb cryptic plasmid. A 4.3-kb plasmid encoding tet(K) and a 2.6-kb plasmid encoding ermC were observed in a few isolates. DNA microarray analysis was used to determine the genetic loci for a series of virulence factors and genes associated with antimicrobial resistance. Comparative genomics between USA300-0114 and three other S. aureus lineages (USA100, USA400, and USA500) defined a set of USA300-0114-specific genes, which may facilitate the strain's pathogenesis within diverse environments.

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mentioning

confidence: 99%

Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States

Tenover¹,

McDougal²,

Goering³

et al. 2006

Journal of Infection

151

221

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“…S. aureus is also the major cause of toxic shock syndrome and scalded skin syndrome (3,4). During the past decade, community acquired, methicillin-resistant strains of S. aureus (CA-MRSA) 3 have caused a marked increase in serious infections in many regions of the United States (5)(6)(7)(8)(9).…”

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confidence: 99%

Knockout of Mkp-1 Enhances the Host Inflammatory Responses to Gram-Positive Bacteria

Wang

Meng

Kuhlman

et al. 2007

The Journal of Immunology

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MAPK phosphatase (MKP)-1 is an archetypal member of the dual specificity protein phosphatase family that dephosphorylates MAPK. We have previously demonstrated that MKP-1 acts as a negative regulator of p38 and JNK in immortalized macrophages after stimulation with peptidoglycan isolated from Gram-positive bacteria. To define the physiological function of MKP-1 during Gram-positive bacterial infection, we studied the innate immune responses to Gram-positive bacteria using Mkp-1 knockout (KO) mice. We found that Mkp-1−/− macrophages exhibited prolonged activation of p38 and JNK, but not of ERK, following exposure to either peptidoglycan or lipoteichoic acid. Compared with wild-type (WT) macrophages, Mkp-1−/− macrophages produced more proinflammatory cytokines such as TNF-α and IL-6. Moreover, after challenge with peptidoglycan, lipoteichoic acid, live or heat-killed Staphylococcus aureus bacteria, Mkp-1 KO mice also mounted a more robust production of cytokines and chemokines, including TNF-α, IL-6, IL-10, and MIP-1α, than did WT mice. Accordingly, Mkp-1 KO mice also exhibited greater NO production, more robust neutrophil infiltration, and more severe organ damage than did WT mice. Surprisingly, WT and Mkp-1 KO mice exhibited no significant difference in either bacterial load or survival rates when infected with live S. aureus. However, in response to challenge with heat-killed S. aureus, Mkp-1 KO mice exhibited a substantially higher mortality rate compared with WT mice. Our studies indicate that MKP-1 plays a critical role in the inflammatory response to Gram-positive bacterial infection. MKP-1 serves to limit the inflammatory reaction by inactivating JNK and p38, thus preventing multiorgan failure caused by exaggerated inflammatory responses.

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“…4 Si bien los genes que codifican la resistencia a meticilina de las cepas hospitalarias y comunitarias son los mismos, el cassette cromosómico estafilocócico en el cual están integrados esos genes es diferente, lo que indica que las cepas de la comunidad no representan una diseminación de las cepas hospitalarias. 12,13 Asimismo, una citotoxina llamada leucocidina Panton-Valentine (PVL) presente en las cepas SAMR-C y en algunas SA meticilino sensibles (SAMS) se asoció a IPPB, 4,14 invasividad, osteomielitis, neumonía necrotizante y mal pronóstico. 4,[14][15][16][17][18] Sin embargo, recientemente, se ha discutido su rol como determinante de virulencia.…”

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Infecciones por Staphylococcus aureus meticilino resistente adquirido en la comunidad: hospitalización y riesgo de letalidad en 10 centros pediátricos de Argentina

Gentile

Bakir

Ensinck

et al. 2018

Arch Argent Pediatr

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Emergence of Community-Associated Methicillin-Resistant Staphylococcus aureus at a Memphis, Tennessee Children??s Hospital

Abstract: Community-associated MRSA has emerged as a potentially invasive pathogen among children in the greater Memphis area, and this phenomenon is not explained by spread of nosocomial strains into the community.

Cited by 168 publications

References 35 publications

Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States

Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States

Knockout of Mkp-1 Enhances the Host Inflammatory Responses to Gram-Positive Bacteria

Infecciones por Staphylococcus aureus meticilino resistente adquirido en la comunidad: hospitalización y riesgo de letalidad en 10 centros pediátricos de Argentina

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