Emergence of committed B lymphoid progenitors in the developing chicken embryo.

Reynaud, Claude‐Agnès; Imhof, Beat A.; Anquez, Viviane; Weill, Jean Claude

doi:10.1002/j.1460-2075.1992.tb05534.x

Cited by 87 publications

(74 citation statements)

References 49 publications

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“…occurs in the fetal liver of most mammals (1) and in the avian yolk sac (2). The repertoire of these B cells becomes further diversified after trafficking to lymphoid tissues such as the bursa of Fabricius in chickens (3), the rabbit appendix (4), the ileal Peyers patches in sheep (5), and other secondary lymphoid organs such as the spleen and lymph nodes in humans and mice.…”

Section: P Rimary Ab Repertoire Development (Vdj Rearrangement)mentioning

confidence: 99%

Antibody Repertoire Development in Fetal and Neonatal Piglets. II. Characterization of Heavy Chain Complementarity-Determining Region 3 Diversity in the Developing Fetus

Butler

Weber

Šinkora

et al. 2000

The Journal of Immunology

104

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Since the actual combinatorial diversity in the VH repertoire in fetal piglets represents <1% of the potential in mice and humans, we wondered whether 1) complementarity-determining region 3 (CDR3) diversity was also restricted; 2) CDR3 diversity changed with fetal age; and 3) to what extent CDR3 contributed to the preimmune VDJ repertoire. CDR3 spectratyping and sequence analyses of 213 CDR3s recovered from >30 fetal animals of different ages showed that >95% of VDJ diversity resulted from junctional diversity. Unlike sheep and cattle, somatic hypermutation does not contribute to the repertoire. These studies also revealed that 1) N region additions are as extensive in VDJ rearrangements recovered at 30 days as those in late term fetuses, suggesting that TdT is fully active at the onset of VDJ rearrangement; 2) nearly 90% of all rearrangement are in-frame until late gestation; 3) the oligoclonal CDR3 spectratype of 30-day fetal liver becomes polyclonal by 50 days, while this change occurs much later in spleen; 4) there is little evidence of individual variation in CDR3 spectratype or differences in spectratype among lymphoid tissues with the exception of the thymus; and 4) there is a tendency for usage of the most JH proximal DH segment (DHB) to decrease in older fetuses and for the longer DH segment to be trimmed to the same length as the shorter DH when used in CDR3. These findings suggest that in the fetal piglet, highly restricted combinatorial diversity and the lack of somatic mutation are compensated by early onset of TdT activity and other mechanisms that contribute to CDR3 junctional diversity.

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Section: P Rimary Ab Repertoire Development (Vdj Rearrangement)mentioning

confidence: 99%

Antibody Repertoire Development in Fetal and Neonatal Piglets. II. Characterization of Heavy Chain Complementarity-Determining Region 3 Diversity in the Developing Fetus

Butler

Weber

Šinkora

et al. 2000

The Journal of Immunology

104

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“…However, the bursa seems not to be essential for the Ig gene rearrangements, which occur before bursal colonization in committed B-cell precursors [2,3]. During the maturation process most of the developing bursal cells die by apoptosis [4][5][6] and only a minority of the daily bursal B-cell production survives to finally emigrate into the periphery [7,8].…”

Section: Introductionmentioning

confidence: 99%

Expression of chL12 Surface Antigen is Associated with Cell Survival in the Avian Bursa of Fabricius

Lampisuo

Liippo

et al. 1998

Scand J Immunol

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Lampisuo M, Arstila TP, Liippo J, Lassila O. Expression of chL12 Surface Antigen is Associated with Cell Survival in the Avian Bursa of Fabricius. Scand J Immunol 1998;47:223-228 During B-cell development in the avian bursa of Fabricius most of the developing B cells die by apoptosis and only a minority survive to emigrate into the periphery. Recently, it has been shown that when developing bursal cells become mature and ready to migrate they start to express chL12 antigen. The expression of this cell-surface molecule was found to be associated with the survival of the bursal cells both after in vitro culture and after in vivo cyclophosphamide (CY) treatment. The frequency of early apoptotic cells in freshly isolated bursal cells was found to be high. The high susceptibility of these cells to apoptosis is in line with the finding of low bcl-2 mRNA expression. We conclude that expression of avian chL12 antigen is associated with the survival of bursal cells.

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“…In chicks, the first B-lymphocyte-committed lineage cells are found in the yolk sac at day 5-6 of development (27), although the earliest identifiable cells with hemopoietic precursor potential are found within the aortic mesoderm at day 3-4 of gestation (28). We hope that the in vitro systems that support T-or B-lymphocyte development from yolk sac cells described here will also be useful to determine the site, origin, and nature of the cells that give rise to yolk sac lymphohemopoietic precursor cells in the mouse embryo.…”

mentioning

confidence: 99%

At day 8-8.5 of mouse development the yolk sac, not the embryo proper, has lymphoid precursor potential in vivo and in vitro.

Palacios

Imhof

1993

Proc. Natl. Acad. Sci. U.S.A.

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At day 8-8.5 of mouse development the yolk sac, not the embryo proper, has lymphoid precursor potential in vivo and in vitro ( ABSTRACTWe have studied both in vitro and in vivo the formation of lymphocyte progenitors before blood circulation (day 9 of gestation) has started in the mouse embryo, and we have determined the tissue where this occurs. The results demonstrate that the yolk sac of embryos at day 8 and day 8.5 of gestation contains precursor cells that can give rise, in vivo and in vito, to mature T and B lymphocytes. No lymphoid precursors were found in the embryo proper at this stage of mouse development. The yolk sac cells with lymphocyte precursor potential are most likely multipotent stem cells rather than cell-lineage-determined T-and/or B-lymphocyte progenitors. The dermed in vitro assays described here that support differentiation of yolk sac stem cells along the T-or B-lymphocyte pathways also may now facilitate the study of the molecular events leading to cell-lineage commitment of lymphocyte progenitors in the mouse embryo.

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Emergence of committed B lymphoid progenitors in the developing chicken embryo.

Cited by 87 publications

References 49 publications

Antibody Repertoire Development in Fetal and Neonatal Piglets. II. Characterization of Heavy Chain Complementarity-Determining Region 3 Diversity in the Developing Fetus

Antibody Repertoire Development in Fetal and Neonatal Piglets. II. Characterization of Heavy Chain Complementarity-Determining Region 3 Diversity in the Developing Fetus

Expression of chL12 Surface Antigen is Associated with Cell Survival in the Avian Bursa of Fabricius

At day 8-8.5 of mouse development the yolk sac, not the embryo proper, has lymphoid precursor potential in vivo and in vitro.

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