Emergence of Clinical Pseudomonas aeruginosa Isolate Guangzhou-PaeC79 Carrying crpP, blaGES-5, and blaKPC-2 in Guangzhou of China

Wang, Linjing; Chen, En-Zhong; Yang, Ling; Feng, Donghua; Xu, Zhenbo; Chen, Dingqiang

doi:10.1089/mdr.2020.0420

Cited by 11 publications

(8 citation statements)

References 20 publications

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“…The 53 studies included in this review were conducted in American, European, and Asian countries. China (14 studies) [ 14 , 15 , 17 , 19 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ], Brazil (10 studies) [ 21 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ], and Colombia (9 studies) [ 22 , 26 , 27 , 28 , 29 , 51 , 52 , 53 , 54 ] were the countries with the most reported studies, followed by Puerto Rico (4 studies) [ 55 , 56 , 57 , 58 ], Chile (2 studies) [ 59 , 60 ], Argentina (2 studies) [ 61 , 62 ], Vietnam (1 study) [ 63 ], Trinidad and Tobago (1 study) [ 64 ], Nepal (1 study) [ 65 ], India (1 study) [ 66 ], Germany (1 study) [ 18 ], Spain (1 study) [ 6 ], and the USA (1 study) […”

Section: Resultsmentioning

confidence: 99%

“…KPC-associated plasmids varied widely in size, from small plasmids of less than 4 Kbp to mega plasmids greater than 400 Kbp [ 19 , 44 ]. Furthermore, it was possible to identify seven different incompatibility groups: IncP [ 50 ], IncP-3-like (IncA/C) [ 19 ], IncU [ 27 , 49 ], IncP-6 [ 17 , 27 , 38 ], IncF-like [ 66 ], IncQ1 [ 21 ], and IncHI1 [ 18 ]. According to the information collected, only 24 bla KPC -carrying P. aeruginosa plasmids were fully sequenced and are available both in the NCBI public database and the published literature ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

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Worldwide Dissemination of blaKPC Gene by Novel Mobilization Platforms in Pseudomonas aeruginosa: A Systematic Review

et al. 2023

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The dissemination of blaKPC-harboring Pseudomonas aeruginosa (KPC-Pa) is considered a serious public health problem. This study provides an overview of the epidemiology of these isolates to try to elucidate novel mobilization platforms that could contribute to their worldwide spread. A systematic review in PubMed and EMBASE was performed to find articles published up to June 2022. In addition, a search algorithm using NCBI databases was developed to identify sequences that contain possible mobilization platforms. After that, the sequences were filtered and pair-aligned to describe the blaKPC genetic environment. We found 691 KPC-Pa isolates belonging to 41 different sequence types and recovered from 14 countries. Although the blaKPC gene is still mobilized by the transposon Tn4401, the non-Tn4401 elements (NTEKPC) were the most frequent. Our analysis allowed us to identify 25 different NTEKPC, mainly belonging to the NTEKPC-I, and a new type (proposed as IVa) was also observed. This is the first systematic review that consolidates information about the behavior of the blaKPC acquisition in P. aeruginosa and the genetic platforms implied in its successful worldwide spread. Our results show high NTEKPC prevalence in P. aeruginosa and an accelerated dynamic of unrelated clones. All information collected in this review was used to build an interactive online map.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Worldwide Dissemination of blaKPC Gene by Novel Mobilization Platforms in Pseudomonas aeruginosa: A Systematic Review

et al. 2023

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“…At present, KPC-producing P . aeruginosa isolates are alarmingly increasingly worldwide, which has aroused the attention and vigilance of clinical researchers [ 44–46 ].…”

Section: Discussionmentioning

confidence: 99%

Emerging and re-emerging KPC-producing hypervirulent Pseudomonas aeruginosa ST697 and ST463 between 2010 and 2021

Zhang

Song

et al. 2022

Emerging Microbes & Infections

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Carbapenem-resistant Pseudomonas aeruginosa (CRPA) has been a major threat to human health due to its increased morbidity and mortality in clinical settings. Carbapenemase genes are less frequently found in CRPA compared with carbapenem-resistant Enterobacterales , of which carbapenemase producers are common. In this study, we identified 11 bla KPC-2 -harbouring P. aeruginosa isolates from 139 carbapenemase-insensitive P . aeruginosa isolates collected between 2010 and 2021 in a tertiary hospital in China. Nine isolates belonged to ST697, while the other two were ST463. The antibiotic susceptibility testing showed that all the isolates were multidrug resistant, including resistance to imipenem, meropenem, ceftazidime, and tigecycline. Patients with Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing P. aeruginosa infections were mostly associated with complicated diseases and prolonged hospital stay, with 30% deterioration. The whole-genome sequencing analysis showed that these isolates carried multiple antibiotic resistance genes and virulence genes, and the KPC-2 genetic elements were highly related in ST697 isolates. The complete sequencing of ST697 isolate SE5416 showed that the harbouring of bla KPC-2 resulted from complex transposition and homologous recombination of an Inc pRBL16 plasmid and other mobile elements. The Galleria mellonella infection model experiment showed that these KPC-2-producing P . aeruginosa –infected larvae had low survival rates and high virulence. The present study revealed the shifting of CRPA from ST697 to ST463 in East China; ST463 had higher drug resistance, posing greater challenges for clinical management.

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“…The pathogenicity of P. aeruginosa stems from diverse virulence factors and remarkable genetic flexibility which allows it to adapt to various habitats and escape host immune defenses ( Klockgether and Tümmler, 2017 ). The emergence of carbapenem-resistant strains of P. aeruginosa is a sanitary health threat ( Wang L. et al, 2021 ; Wang L. J. et al, 2021 ; Liu et al, 2022c ) and a source of extreme concern to the World Health Organization (WHO). In 2007, the number of infections arising from P. aeruginosa , which is multidrug- or carbapenem-resistant, was approximately 23,575 and the number of deaths was approximately 1,573, according to reports from the EU region.…”

Section: Pseudomonas Aeruginosa Is a Leading Human Pathogen ...mentioning

confidence: 99%

Pseudomonas aeruginosa: A typical biofilm forming pathogen and an emerging but underestimated pathogen in food processing

Huang

et al. 2023

Front. Microbiol.

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Pseudomonas aeruginosa (P. aeruginosa) is a notorious gram-negative pathogenic microorganism, because of several virulence factors, biofilm forming capability, as well as antimicrobial resistance. In addition, the appearance of antibiotic-resistant strains resulting from the misuse and overuse of antibiotics increases morbidity and mortality in immunocompromised patients. However, it has been underestimated as a foodborne pathogen in various food groups for instance water, milk, meat, fruits, and vegetables. Chemical preservatives that are commonly used to suppress the growth of food source microorganisms can cause problems with food safety. For these reasons, finding effective, healthy safer, and natural alternative antimicrobial agents used in food processing is extremely important. In this review, our ultimate goal is to cover recent advances in food safety related to P. aeruginosa including antimicrobial resistance, major virulence factors, and prevention measures. It is worth noting that food spoilage caused by P. aeruginosa should arouse wide concerns of consumers and food supervision department.

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Emergence of Clinical Pseudomonas aeruginosa Isolate Guangzhou-PaeC79 Carrying crpP, bla_GES-5, and bla_KPC-2 in Guangzhou of China

Cited by 11 publications

References 20 publications

Worldwide Dissemination of blaKPC Gene by Novel Mobilization Platforms in Pseudomonas aeruginosa: A Systematic Review

Worldwide Dissemination of blaKPC Gene by Novel Mobilization Platforms in Pseudomonas aeruginosa: A Systematic Review

Emerging and re-emerging KPC-producing hypervirulent Pseudomonas aeruginosa ST697 and ST463 between 2010 and 2021

Pseudomonas aeruginosa: A typical biofilm forming pathogen and an emerging but underestimated pathogen in food processing

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