Emergence of blaNDM– 1-Carrying Aeromonas caviae K433 Isolated From Patient With Community-Acquired Pneumonia

Abstract

To demonstrate the detailed genetic characteristics of a blaNDM–1-carrying multidrug-resistant Aeromonas caviae strain, the complete genome of the A. caviae strain K433 was sequenced by Illumina HiSeq and Oxford nanopore platforms, and mobile genetic elements associated with antibiotic resistance genes were analyzed by a series of bioinformatics methods. A. caviae K433 which was determined to produce class B carbapenemase, was resistant to most antibiotics tested except amikacin. The genome of K433 consisted of a chromosome cK433 (6,482-kb length) and two plasmids: pK433-qnrS (7.212-kb length) and pK433-NDM (200.855-kb length), the last being the first investigated blaNDM-carrying plasmid from Aeromonas spp. By comparison of the backbone and MDR regions from the plasmids studied, they involved a highly homologous sequence structure. This study provides in-depth genetic insights into the plasmids integrated with blaNDM-carrying genetic elements from Aeromonas spp.

Genetic characterization and virulence determinants of multidrug-resistant NDM-1-producing Aeromonas caviae

“…Currently, two reports related to bla NDM–1 -harboring A. caviae are available and both were isolated from the clinic in China. However, they were not associated with a biliary tract infection ( Luo et al, 2022 ; Xu et al, 2022 ). In addition, FAHZZU2447 carries other drug-resistance genes, including beta-lactams ( bla TEM– 1 B , bla NDM–1 , bla MOX– 6 , and bla OXA– 18 ), aminoglycosides [ aac(3)-IId , aph(6)-Id , aph(3 )-Ib ], tetracyclines [ tet(A) ], phenicols ( catA1 ), and sulfonamide ( dfrA5 , sul1 , sul2 ).…”

“…However, the current spread of NDM-1 was related to Enterobacterales and limited reports are available of NDM-1-carrying Aeromonas spp. ( Luo et al, 2022 ; Xu et al, 2022 ). To date, only two studies have reported on NDM-1-producing A. caviae , which were isolated from sputum and blood, and bla NDM–1 genes were located on the plasmid and chromosome, respectively ( Luo et al, 2022 ; Xu et al, 2022 ).…”

“…( Luo et al, 2022 ; Xu et al, 2022 ). To date, only two studies have reported on NDM-1-producing A. caviae , which were isolated from sputum and blood, and bla NDM–1 genes were located on the plasmid and chromosome, respectively ( Luo et al, 2022 ; Xu et al, 2022 ). The emergence of NDM-1-harboring A. caviae in the clinic is concerning.…”

Strand-biased circularizing integrative elements spread tmexCD-toprJ gene clusters encoding RND-type multidrug efflux pumps by repeated transpositions

“…caviae K333 (accession no. CP084031 ) isolated from a human in China in 2020 (20). 5’- and 3’-ends of SEs and their 6-bp transposition footprint sequences in Aeromonas species isolates in this study are shown.…”

“…CP039832 ) (19) and A. caviae K333 (accession no. CP084031 ) (20), respectively. Moreover, the tet (X4) - containing region in NUITM-VA1 was also detected in the WCW1-2 chromosome, suggesting that these genetic structures around ARGs in Aeromonas species isolates herein described are not an unusual event.…”