Emergence of Antibiotic Resistance Nano Enzyme in Staphylococcus species Isolated from Clinical, Biotic and Abiotic Conditions

Abstract

Health care workers and hospital surfaces have important role in nosocomial infection, if resistance strain transfer to hospitalized patients, led to spread of antibiotic nosocomial infections. The research was laboratory and performed during 2005/2007 years in Azzahrahospital in Isfahan. According to statistical formula study on147 Staphylococcus species isolated from clinical samples, skin hands of health care workersandhospital surfaces. Bacterial identification, were performed with microbiological methods and bet lactamase product was performed with Acidometric method. According to Acidometric result 100% and 50% of S. aureus and S. epidermidis isolated from clinical samples and 75% and 66.6% of S. aureus and S. epidermidis isolated from skin hands of health care worker and 84.6%, 70.45% and 75% of S. aureus, S. epidermidis and S. saprophyticus isolated from hospital surfaces can produce beta lactamase Nano enzyme. Result demonstrate high prevalence of resistance antibiotics Staphylococcusspp. isolated from clinical, biotic and abiotic conditions in hospital. One of reason creative antibiotic resistant in bacteria is increase contact of sensitive bacteria with resistance strains.