Emergence of a novel multidrug-resistant Pseudomonas aeruginosa strain producing IMP-type metallo-β-lactamases and AAC(6′)-Iae in Japan

Kitao, Tomoe; Tada, Tatsuya; Tanaka, Masashi; Narahara, Kenji; Shimojima, Masayuki; Shimada, Kayo; Miyoshi‐Akiyama, Tohru; Kirikae, Teruo

doi:10.1016/j.ijantimicag.2012.01.020

Cited by 30 publications

(24 citation statements)

References 13 publications

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“…P. aeruginosa belonging to ST235 has spread in Asian countries, including India, South Korea, Malaysia, Sri Lanka, Thailand, and Taiwan (3). In particular, 88.3% of multidrug-resistant P. aeruginosa strains obtained in medical settings in Japan belonged to ST235 (31).…”

Section: Resultsmentioning

confidence: 99%

Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam

Tada

Nhung

Miyoshi‐Akiyama

et al. 2016

Antimicrob Agents Chemother

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Forty clinical isolates of multidrug-resistant Pseudomonas aeruginosa were obtained in a medical setting in Hanoi, Vietnam. Whole genomes of all 40 isolates were sequenced by MiSeq (Illumina), and phylogenic trees were constructed from the single nucleotide polymorphism concatemers. Of these 40 isolates, 24 (60.0%) harbored metallo-␤-lactamase-encoding genes, including bla IMP-15 , bla IMP-26 , bla IMP-51 , and/or bla NDM-1 . Of these 24 isolates, 12 harbored bla IMP-26 and belonged to sequence type 235 (ST235). Escherichia coli expressing bla IMP-26 was significantly more resistant to doripenem and meropenem than E. coli expressing bla IMP-1 and bla IMP-15 . IMP-26 showed higher catalytic activity against doripenem and meropenem than IMP-1 and against all carbapenems tested, including doripenem, imipenem, meropenem, and panipenem, than did IMP-15. These data suggest that clinical isolates of multidrug-resistant ST235 P. aeruginosa producing IMP-26 with increased carbapenem-hydrolyzing activities are spreading in medical settings in Vietnam.

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Section: Resultsmentioning

confidence: 99%

Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam

Tada

Nhung

Miyoshi‐Akiyama

et al. 2016

Antimicrob Agents Chemother

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“…ST235 is an international clone detected in Europe (31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41), Russia (42), Asia (7,(43)(44)(45)(46)(47)(48)(49), and the Middle East (50). ST235, ST111, and ST175 are regarded as epidemic high-risk clones, and ST235 lacks pyocyanin and pyoverdine production (51).…”

Section: Figmentioning

confidence: 99%

“…ST235, ST111, and ST175 are regarded as epidemic high-risk clones, and ST235 lacks pyocyanin and pyoverdine production (51). The association of ST235 with a variety of carbapenem resistance acquired by horizontal transfer of the genes such as bla PER (31,36,40,41,50), bla OXA (36,43,50), bla GES (31,39), bla SPM (36), bla VIM (31-34, 36-38, 42, 44, 52), bla IMP (36,(43)(44)(45)(46)(47)(48)(49), bla FIM (53), and bla NDM (54) suggests its importance in public health and in P. aeruginosa's flexibility in adaptation. Recently, IMP-6-producing P. aeruginosa ST235 has become endemic in Korea (48).…”

Section: Figmentioning

confidence: 99%

Persistence and Epidemic Propagation of a Pseudomonas aeruginosa Sequence Type 235 Clone Harboring an IS 26 Composite Transposon Carrying the bla _IMP-1 Integron in Hiroshima, Japan, 2005 to 2012

Shimizu

Kayama

Kouda

et al. 2015

Antimicrob Agents Chemother

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A 9-year surveillance for multidrug-resistant (MDR) Pseudomonas aeruginosa in the Hiroshima region showed that the number of isolates harboring the metallo-␤-lactamase gene bla IMP-1 abruptly increased after 2004, recorded the highest peak in 2006, and showed a tendency to decline afterwards, indicating a history of an epidemic. PCR mapping of the variable regions of the integrons showed that this epidemic was caused by the clonal persistence and propagation of an MDR P. aeruginosa strain harboring the bla IMP-1 gene and an aminoglycoside 6=-N-acetyltransferase gene, aac(6=)-Iae in a class I integron (In113), whose integrase gene intl1 was disrupted by an IS26 insertion. Sequence analysis of the representative strain PA058447 resistance element containing the In113-derived gene cassette array showed that the element forms an IS26 transposon embedded in the chromosome. It has a Tn21 backbone and is composed of two segments sandwiched by three IS26s. In Japan, clonal nationwide expansion of an MDR P. aeruginosa NCGM2.S1 harboring chromosomally encoded In113 with intact intl1 is reported. Multilocus sequence typing and genomic comparison strongly suggest that PA058447 and NCGM2.S1 belong to the same clonal lineage. Moreover, the structures of the resistance element in the two strains are very similar, but the sites of insertion into the chromosome are different. Based on tagging information of the IS26 present in both resistance elements, we suggest that the MDR P. aeruginosa clone causing the epidemic in Hiroshima for the past 9 years originated from a common ancestor genome of PA058447 and NCGM2.S1 through an IS26 insertion into intl1 of In113 and through IS26-mediated genomic rearrangements. P seudomonas aeruginosa is an opportunistic human pathogen and a leading cause of nosocomial infections. It also causes mastitis in dairy cows as mild chronic persistent inflammation (1). Carbapenems have been used to treat P. aeruginosa infections; however, P. aeruginosa isolates resistant to carbapenems are reported in many countries and an acquired resistance to carbapenems by metallo-␤-lactamase (MBL) genes causes a serious therapeutic problem in clinical settings. Carbapenem-resistant P. aeruginosa strains producing MBLs were first discovered in Japan (2, 3) and have since been reported in many countries all over the world (4).Specifically, IMP, VIM, SPM, and NDM are the most important types of MBL with worldwide distribution, and their genes are frequently carried on class 1 integrons (4, 5). Class 1 integrons are often found on plasmids or as a part of transposons or resistance genetic elements that are able to integrate into chromosomal elements, resulting in an increase in the number of resistant Gramnegative bacilli (6). MBL-producing P. aeruginosa strains often behave as multidrug-resistant (MDR) strains. The recent increase of nosocomial infections caused by MDR P. aeruginosa has raised a serious concern.In the Hiroshima prefecture, we have conducted a multihospital surveillance for drug-resistant P. aeruginosa i...

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“…The emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa strains, defined as having resistance to all carbapenems, amikacin, and fluoroquinolones, has become a serious problem in medical settings in Japan (Kirikae et al, 2008;Sekiguchi et al, 2007;Sekiguchi et al, 2005;Kitao et al, 2012).…”

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confidence: 99%

“…Approximately 70% of MDR P. aeruginosa isolates in Japan produced AAC(6′)-Iae (Kitao et al, 2012). We first developed an immunochromatographic assay to detect AAC(6′)-Iae (Kitao et al, 2010).…”

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confidence: 99%

Development of an immunochromatographic assay for rapid detection of AAC(6′)-Ib-producing Pseudomonas aeruginosa

Tada

Miyoshi‐Akiyama

Tanaka

et al. 2012

Journal of Microbiological Methods

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Emergence of a novel multidrug-resistant Pseudomonas aeruginosa strain producing IMP-type metallo-β-lactamases and AAC(6′)-Iae in Japan

Cited by 30 publications

References 13 publications

Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam

Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam

Persistence and Epidemic Propagation of a Pseudomonas aeruginosa Sequence Type 235 Clone Harboring an IS 26 Composite Transposon Carrying the bla _IMP-1 Integron in Hiroshima, Japan, 2005 to 2012

Development of an immunochromatographic assay for rapid detection of AAC(6′)-Ib-producing Pseudomonas aeruginosa

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Emergence of a novel multidrug-resistant Pseudomonas aeruginosa strain producing IMP-type metallo-β-lactamases and AAC(6′)-Iae in Japan

Cited by 30 publications

References 13 publications

Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam

Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam

Persistence and Epidemic Propagation of a Pseudomonas aeruginosa Sequence Type 235 Clone Harboring an IS 26 Composite Transposon Carrying the bla IMP-1 Integron in Hiroshima, Japan, 2005 to 2012

Development of an immunochromatographic assay for rapid detection of AAC(6′)-Ib-producing Pseudomonas aeruginosa

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Persistence and Epidemic Propagation of a Pseudomonas aeruginosa Sequence Type 235 Clone Harboring an IS 26 Composite Transposon Carrying the bla _IMP-1 Integron in Hiroshima, Japan, 2005 to 2012