Emergence of a New Strain of DENV-2 in South America: Introduction of the Cosmopolitan Genotype through the Brazilian-Peruvian Border

Amorim, Murilo Tavares; Hernández, Leonardo Henrique Almeida; Naveca, Felipe Gomes; Prazeres, Ivy Tsuya Essashika; Wanzeller, Ana Lucia Monteiro; Silva, Eliana Vieira Pinto da; Casseb, Lívia Medeiros Neves; Silva, Fabio Silva da; Silva, Sandro Patroca da; Nunes, Bruno Tardelli Diniz; Cruz, Ana Cecília Ribeiro

doi:10.20944/preprints202305.1014.v1

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…Finally, in other states including AM and RS, where genomic surveillance detected recent introductions of DENV-2-GII in 2022, DENV-1 was responsible for most dengue cases in the whole period supporting that DENV-2-GII only caused localized outbreaks in those states. In fact, in 2022, DENV-1 was responsible for more than 80% of the dengue cases in 17 out of 25 Brazilian states with data available.In summary, this study confirms that DENV-2-GII is circulating nationally in Brazil, expanding the findings of previous localized studies6,7 . Our phylogenetic analysis unveiled multiple introductions of this genotype into Brazil from Peru, generating multiple clades that spread in the country with different success.…”

supporting

confidence: 89%

“…Here we present new data on the DENV- 1) which were aligned with a DENV-2-GII representative global dataset. Maximum likelihood phylogenetic analysis revealed that all DENV-2-GII Brazilian genomes, from this and previous studies 6,7 , clustered in a monophyletic clade with sequences from Peru and Bangladesh (Supplementary Figure 1). Our To assess the potential of DENV-2-GII to spread and trigger relevant outbreaks in Brazil, we analyzed the dynamics of DENV serotypes' circulation in the country as identified through PCR tests by the laboratory surveillance and informed in the national system of diseases of compulsory notification (SINAN).…”

Section: Research Lettersupporting

confidence: 63%

“…In 2021, the first cases of DENV-2 Cosmopolitan genotype (DENV-2-GII) were identified in the Brazilian states of Goiás (GO) 6 and Acre (AC) 7 , located in the Central-Western and Northern country regions, respectively. Phylogenetic analysis suggested that the Brazilian DENV-2-GII sequences derived from an outbreak of this genotype in Peru in 2019 8 , representing the first documented introduction of DENV-2-GII in the Americas.…”

Section: Research Lettermentioning

confidence: 99%

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Multiple introductions and country-wide spread of DENV-2 genotype II (Cosmopolitan) in Brazil

Gräf,

Ferreira,

de Lima

et al. 2023

Preprint

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Dengue virus serotype 2, genotype Cosmopolitan (DENV-2-GII) is one of the most widespread DENV strains in the globe. However, in the Americas, the first cases of DENV-2-GII were only described in 2019, in Peru, and in 2021 in the Brazilian states of Goias and Acre. To gain new information about the circulation of DENV-2-GII in Brazil, we sequenced 237 DENV-2 confirmed cases sampled between March 2021 and March 2023 and revealed that genotype II is already present in all geographic regions of Brazil. Phylogenetic analysis inferred that DENV-2-GII was introduced at least four times in the country, between May 2020 and August 2022, generating multiple clades that spread throughout the country with different success. Analyzing country-wide laboratory surveillance data showed that between 2018 and 2022, the Brazilian dengue epidemic was dominated by DENV-1 and DENV-2, with significant regional differences. This complex scenario might have created an heterogeneous population immune landscape, which could impose local barriers against transmission of DENV-2-GII in Brazil, leading to sustained cryptic circulation and localized outbreaks. In summary, our study stresses the importance of arboviral genomic surveillance to close monitoring and better understand the potential impact of DENV-2-GII in the coming years.

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supporting

confidence: 89%

Section: Research Lettersupporting

confidence: 63%

Section: Research Lettermentioning

confidence: 99%

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Multiple introductions and country-wide spread of DENV-2 genotype II (Cosmopolitan) in Brazil

Gräf,

Ferreira,

de Lima

et al. 2023

Preprint

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Exploring Microorganisms Associated to Acute Febrile Illness and Severe Neurological Disorders of Unknown Origin: A Nanopore Metagenomics Approach

Moreno,

de Andrade,

de Melo Iani

et al. 2024

Genes

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Acute febrile illness (AFI) and severe neurological disorders (SNDs) often present diagnostic challenges due to their potential origins from a wide range of infectious agents. Nanopore metagenomics is emerging as a powerful tool for identifying the microorganisms potentially responsible for these undiagnosed clinical cases. In this study, we aim to shed light on the etiological agents underlying AFI and SND cases that conventional diagnostic methods have not been able to fully elucidate. Our approach involved analyzing samples from fourteen hospitalized patients using a comprehensive nanopore metagenomic approach. This process included RNA extraction and enrichment using the SMART-9N protocol, followed by nanopore sequencing. Subsequent steps involved quality control, host DNA/cDNA removal, de novo genome assembly, and taxonomic classification. Our findings in AFI cases revealed a spectrum of disease-associated microbes, including Escherichia coli, Streptococcus sp., Human Immunodeficiency Virus 1 (Subtype B), and Human Pegivirus. Similarly, SND cases revealed the presence of pathogens such as Escherichia coli, Clostridium sp., and Dengue virus type 2 (Genotype-II lineage). This study employed a metagenomic analysis method, demonstrating its efficiency and adaptability in pathogen identification. Our investigation successfully identified pathogens likely associated with AFI and SNDs, underscoring the feasibility of retrieving near-complete genomes from RNA viruses. These findings offer promising prospects for advancing our understanding and control of infectious diseases, by facilitating detailed genomic analysis which is critical for developing targeted interventions and therapeutic strategies.

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Detection of a Multiple Circulation Event of Dengue Virus 2 Strains in the Northern Region of Brazil

Amorim,

Naveca,

Hernández

et al. 2024

TropicalMed

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Dengue virus serotype 2 (DENV-2) is responsible for dengue epidemics on a global scale and is associated with severe cases of the disease. This study conducted a phylogenetic investigation of DENV-2 isolates from 2017 to 2021 originating from the northern states of Brazil. A total of 32 samples from DENV-2 isolates were analyzed, including 12 from Acre, 19 from Roraima, and one from Tocantins. Only one lineage of the Asian-American genotype and one lineage of the cosmopolitan genotype were observed: Lineage 1, Asian-American genotype (connection to Puerto Rico); Lineage 5, cosmopolitan genotype (connection to Peru). Our results provide important data regarding the study of DENV genotypes and lineage distribution and open up possibilities for probable introduction and dissemination routes.

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Emergence of a New Strain of DENV-2 in South America: Introduction of the Cosmopolitan Genotype through the Brazilian-Peruvian Border

Abstract: We hypothesize the possible route of introduction of the cosmopolitan DENV-2 genotype into Brazil through the border with Peru. The five sequences presented in this study create a geographical link with the three others sequences of this genotype already recorded on the continent.

Cited by 3 publications

References 21 publications

Multiple introductions and country-wide spread of DENV-2 genotype II (Cosmopolitan) in Brazil

Multiple introductions and country-wide spread of DENV-2 genotype II (Cosmopolitan) in Brazil

Exploring Microorganisms Associated to Acute Febrile Illness and Severe Neurological Disorders of Unknown Origin: A Nanopore Metagenomics Approach

Detection of a Multiple Circulation Event of Dengue Virus 2 Strains in the Northern Region of Brazil

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