Emergence of a Hybrid Surface Phenotype in Mixed Blast Crisis of Chronic Granulocytic Leukaemia

Hagner, G.; Kölmel, Hans Wolfgang; Wirthmüller, R.

doi:10.1159/000206767

Cited by 6 publications

(2 citation statements)

References 8 publications

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“…These observations are consistent with the emergence of either hybrid [22] biphenotypic clones [23][24][25][26] as terminal events in the natural history of chronic granulocytic leukaemia. In addition, the clonal evolution that was superimposed upon the persisting Philadelphia chromosome during the first transformation was reversed by chemother apy, emphasizing the importance of recognising the existence of treatment-responsive leukaemic transfor mations.…”

Section: Introductionsupporting

confidence: 84%

“…The persistence of the Philadelphia chromosome positive clone, with the superimposition of, firstly, a lymphoblastic, secondly, a hybrid or phenotypic and, thirdly, a megakaryoblastic tumour, illustrates the variable potential that a haematopoietic stem cell has for differentiation [22][23][24][25][26]. Furthermore, in the first blastic transformation, there was clonal evolution su perimposed upon the classical 9:22 translocation and the reversal of these changes with successful treatment suggests that the cytogenetic changes could reason ably be considered markers for the new clone and their eradication a reflection of effective chemother apy.…”

Section: Resultsmentioning

confidence: 99%

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Multiple Blastic Transformations in the Course of Chronic Granulocytic Leukaemia

Roux¹,

Jacobs²

1986

Acta Haematol

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A 14-year-old boy with Philadelphia chromosome-positive chronic granulocytic leukaemia underwent morphologically distinctive blastic transformation on 3 separate occasions. The first was clearly lymphoblastic, the second was associated with meningeal leukaemia and was morphologically biphenotypic, expressing mixed myeloid and lymphoid features, while the third was characterised by extreme thrombocytosis and megakaryoblastic proliferation. The classical t (9:22) Philadelphia chromosome remained constant throughout the illness, but the additional cytogenetic changes that occurred with the first transformation were abolished with response to conventional therapy for lymphoblastic leukaemia. A total of 19 months of good-quality life was achieved, but on the third occasion, the boy died from intracranial haemorrhage before treatment could be initiated.

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Section: Introductionsupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Multiple Blastic Transformations in the Course of Chronic Granulocytic Leukaemia

Roux¹,

Jacobs²

1986

Acta Haematol

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Dual expression of lymphoid/basophil markers on single blast cells transformed from chronic myeloid leukemia

et al. 1986

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Incidence of involvement of the B and T lymphocyte lineages in chronic myelogenous leukemia

Nitta

Kato

Strife

et al. 1985

Blood

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Peripheral blood specimens were obtained from 22 patients with Philadelphia chromosome (Ph1) positive chronic myelogenous leukemia (CML) (16 in chronic phase, 2 in an accelerated phase, and 4 in blast crisis). Studies were performed to determine the frequency of the presence of the Ph1 chromosome in cells of lymphoid lineages. Rosetted (E+) lymphocytes (T lymphocytes) from nine patients in chronic phase and one patient in blast crisis were stimulated with T cell growth factor interleukin 2 (IL-2) and/or phytohemagglutinin (PHA). All ten patients had sufficient T lymphocyte metaphases for analysis and of a total of 461 metaphases examined, only one contained the Ph1 chromosome. Nucleated cells of density less than 1.077 g/mL were infected with Epstein-Barr virus (EBV). Following infection, cell lines were established from individual colonies attached to egg albumin- coated Lab-Tek slide chambers (clonal cell lines) or from suspension culture in 96-well tissue culture cluster dishes (nonclonal cell lines). Cell surface and intracellular marker analysis confirmed the B lymphocyte phenotype of all the cell lines examined. B lymphoblastoid cell lines were established from 16 of the 22 patients. All lines from 12 patients were Ph1-negative. From two chronic phase patients, both Ph1-positive and Ph1-negative lines were established. From one patient in an accelerated phase, only Ph1-positive lines were established. From another patient in blast crisis (of myeloblastic phenotype), only Ph1- positive lines were established initially; however, five months later, after the patient had been treated with mitoxantrone, only Ph1-negative lines were derived from this patient. Based on these results, it appears that most B cells and mature T cells in most CML patients are Ph1-negative, but that about 25% of patients have predominantly Ph1- positive B cells or a mixture of Ph1-positive and Ph1-negative B cells that are capable of growing as established cell lines after transformation with EBV.

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Emergence of a Hybrid Surface Phenotype in Mixed Blast Crisis of Chronic Granulocytic Leukaemia

Cited by 6 publications

References 8 publications

Multiple Blastic Transformations in the Course of Chronic Granulocytic Leukaemia

Multiple Blastic Transformations in the Course of Chronic Granulocytic Leukaemia

Dual expression of lymphoid/basophil markers on single blast cells transformed from chronic myeloid leukemia

Incidence of involvement of the B and T lymphocyte lineages in chronic myelogenous leukemia

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