Emergence and plasmid cointegration-based evolution of NDM-1-producing ST107 Citrobacter freundii high-risk resistant clone in China

Liu, Haiyang; Tu, Yuexing; He, Jintao; Xu, Qingye; Zhang, Xiaofan; Mu, Xinli; Chen, Minhua; Zhou, Hua; Li, Xi

doi:10.1016/j.ijantimicag.2023.107069

Cited by 2 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…25,26 It has been found that the majority of bla NDM was located on plasmids, and due to the transferability of plasmids, the horizontal spread of plasmid-mediated resistance genes may occur if strict isolation measures are not taken. 27 The plasmid conjugation transfer experiments in this study demonstrated that 13 CREC isolates were capable of transferring plasmids to EC600 recipient bacteria, conferring resistance and promoting the development of multidrug-resistant bacteria. This finding aligns with a study conducted in other southwestern regions of China.…”

Section: Discussionmentioning

confidence: 65%

Carbapenem-Resistant Enterobacter cloacae Complex in Southwest China: Molecular Characteristics and Risk Factors Caused by NDM Producers

Zeng,

Wei,

et al. 2024

IDR

View full text Add to dashboard Cite

The isolation rate of carbapenem-resistant Enterobacter cloacae complex (CREC) is continuously increasing. The aims of this study were to investigate the molecular characteristics and risk factors associated with CREC infections. Methods: Bacterial species were identified using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) (Bruker Daltonik GmbH, Bremen, Germany), and the hsp60 gene was utilized for further typing. Antimicrobial susceptibilities were assessed through the MicroScan WalkAway 96 Plus system (Siemens, Germany) and the microbroth dilution method. Antimicrobial resistance genes were screened through polymerase chain reaction (PCR), while the homologous relationship was assessed using multilocus sequence typing (MLST). Conjugation experiments were performed to verify whether the plasmid could be transferred. Additionally, logistic regression model was employed to analyze risk factors for CREC infections. Results: 32 strains of CREC bacteria were isolated during the study, yet only 20 were retained for preservation. While the isolates demonstrated resistance to the majority of antibiotics, they exhibited high sensitivity to polymyxin B and tigecycline. All isolates carried the bla NDM resistance gene, including 13 bla NDM-1 isolates and 7 bla NDM-5 isolates. MLST homology analysis revealed the presence of seven known ST types and one new ST type. Conjugation experiments confirmed that 13 isolates were capable of transferring the bla NDM resistance gene to Escherichia coli strain EC600. Single-factor analysis identified multiple primary risk factors for CREC infection, but multivariate analysis did not reveal independent risk factors. Conclusion:This study investigates the molecular characteristics and risk factors associated with CREC infections. The detection rate of CREC strains in our hospital is continuously rising and homology analysis suggested that strains might spread in our hospital, emphasizing the importance of implementing effective preventive measures to control the horizontal transmission of plasmid-mediated antimicrobial resistance genes.

show abstract

Section: Discussionmentioning

confidence: 65%

Carbapenem-Resistant Enterobacter cloacae Complex in Southwest China: Molecular Characteristics and Risk Factors Caused by NDM Producers

Zeng,

Wei,

et al. 2024

IDR

View full text Add to dashboard Cite

show abstract

“…Escherichia coli ( E. coli ), Klebsiella pneumoniae ( K. pneumoniae ), Enterococcus faecium ( E. faecium ), Enterococcus faecalis ( E. faecalis ) are the most common pathogens in UTIs, 30 while KPC and NDM are the most important carbapenem-resistant genes in E. coli and K. pneumoniae . 31,32 Therefore, these four pathogens and two antibiotic-resistant genes were used as examples for the method validation (Fig. S1B†).…”

Section: Methodsmentioning

confidence: 99%

FARPA-based tube array coupled with quick DNA extraction enables ultra-fast bedside detection of antibiotic-resistant pathogens

Huang,

Yue,

Wei

et al. 2024

Analyst

View full text Add to dashboard Cite

show abstract

Emergence and plasmid cointegration-based evolution of NDM-1-producing ST107 Citrobacter freundii high-risk resistant clone in China

Cited by 2 publications

References 40 publications

Carbapenem-Resistant Enterobacter cloacae Complex in Southwest China: Molecular Characteristics and Risk Factors Caused by NDM Producers

Carbapenem-Resistant Enterobacter cloacae Complex in Southwest China: Molecular Characteristics and Risk Factors Caused by NDM Producers

FARPA-based tube array coupled with quick DNA extraction enables ultra-fast bedside detection of antibiotic-resistant pathogens

Contact Info

Product

Resources

About