Emergence and Evolution of Unique Plasmids Harboring
 bla
 IMP-70
 and
 bla
 CTX-M-253
 in Multidrug-Resistant Providencia rettgeri

Watanabe, Mako; Nakano, Ryuichi; Tanouchi, Ayako; Nakano, Akiyo; Suzuki, Yuki; Saito, Kai; Sakata, Ryuji; Ogawa, Miho; Yano, Hisakazu

doi:10.1128/spectrum.01204-22

Cited by 3 publications

(1 citation statement)

References 24 publications

(26 reference statements)

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“…bla IMP-70 and bla CTX-M-253 in multidrug-resistant Providencia Rettgeri and suggested that the cointegration of plasmids in P . rettgeri may not be unusual and may play a role in the transmission of clinically relevant b-lactamases ( Watanabe et al, 2022 ). In our study, one class 1 integron was identified in p18004577_NDM, which harbored multiple resistance genes, such as bla O XA -10 , ant ( 2′ ) -Ia , catB8 , bla PER-4 , and two Δsul1 .…”

Section: Discussionmentioning

confidence: 99%

Conjugation of plasmid harboring blaNDM-1 in a clinical Providencia rettgeri strain through the formation of a fusion plasmid

Zhang

Wang

et al. 2023

Front. Microbiol.

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Providencia rettgeri has recently gained increased importance owing to the New Delhi metallo-β-lactamase (NDM) and other β-lactamases produced by its clinical isolates. These enzymes reduce the efficiency of antimicrobial therapy. Herein, we reported the findings of whole-genome sequence analysis and a comprehensive pan-genome analysis performed on a multidrug-resistant P. rettgeri 18004577 clinical strain recovered from the urine of a hospitalized patient in Shandong, China, in 2018. Providencia rettgeri 18004577 was found to have a genome assembly size of 4.6 Mb with a G + C content of 41%; a circular plasmid p18004577_NDM of 273.3 Kb, harboring an accessory multidrug-resistant region; and a circular, stable IncT plasmid p18004577_Rts of 146.2 Kb. Additionally, various resistance genes were identified in its genome, including blaNDM-1, blaOXA-10, blaPER-4, aph(3′)-VI, ant(2′′)-Ia, ant(3′)-Ia, sul1, catB8, catA1, mph(E), and tet. Conjugation experiments and whole-genome sequencing revealed that the blaNDM-1 gene could be transferred to the transconjugant via the formation of pJ18004577_NDM, a novel hybrid plasmid. Based on the genetic comparison, the main possible formation process for pJ18004577_NDM was the insertion of the [ΔISKox2-IS26-ΔISKox2]-aph(3′)-VI-blaNDM-1 translocatable unit module from p18004577_NDM into plasmid p18004577_Rts in the Russian doll insertion structure (ΔISKox2-IS26-ΔISKox2), which played a role similar to that of IS26 using the “copy-in” route in the mobilization of [aph(3′)-VI]-blaNDM-1. The array, multiplicity, and diversity of the resistance and virulence genes in this strain necessitate stringent infection control, antibiotic stewardship, and periodic resistance surveillance/monitoring policies to preempt further horizontal and vertical spread of the resistance genes. Roary analysis based on 30 P. rettgeri strains pan genome identified 415 core, 756 soft core, 5,744 shell, and 12,967 cloud genes, highlighting the “close” nature of P. rettgeri pan-genome. After a comprehensive pan-genome analysis, representative biological information was revealed that included phylogenetic distances, presence or absence of genes across the P. rettgeri bacteria clade, and functional distribution of proteins. Moreover, pan-genome analysis has been shown to be an effective approach to better understand P. rettgeri bacteria because it helps develop various tailored therapeutic strategies based on their biological similarities and differences.

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Section: Discussionmentioning

confidence: 99%

Conjugation of plasmid harboring blaNDM-1 in a clinical Providencia rettgeri strain through the formation of a fusion plasmid

Zhang

Wang

et al. 2023

Front. Microbiol.

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Identification of a novel Providencia species showing multi-drug-resistant in three patients with hospital-acquired infection

Yang,

Chen,

Yang

et al. 2024

International Journal of Antimicrobial Agents

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Hepatic failure with Providencia rettgeri infection: case report

Yan,

Xi,

Han

et al. 2024

Preprint

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Background Providencia rettgeri is an uncommon opportunistic pathogen of nosocomial infection in humans. It often occurs in urinary tract infection and skin damage.Few reports find providencia rettgeri infection in a hepatic failure patient. Case presentation: A 29 years old man admitted to us with crushing injury by a falling objects in construction site, which caused hepatorrhexis and hepatic failure .In the therapy period of infective intensive care unit (ICU), the patient got tracheal intubation to support breath and the rinse of fiber bronchoscopy cultured providencia rettgeri . Conclusions Providencia rettgeri is associated with the patients with totally terribble health conditionos, like post-enormous sugeries,large area skin burn and liver failure and so on. Few clincal cases also find the patients with assisted ventilation get infected with providencia rettgeri. And due to the pathogen’s character of antimicrobial resistance,it rasies mortality and prolongs recovery time. Thereafter,attention should be taken to those patients in their recovery period.

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Emergence and Evolution of Unique Plasmids Harboring bla _IMP-70 and bla _CTX-M-253 in Multidrug-Resistant Providencia rettgeri

Cited by 3 publications

References 24 publications

Conjugation of plasmid harboring blaNDM-1 in a clinical Providencia rettgeri strain through the formation of a fusion plasmid

Conjugation of plasmid harboring blaNDM-1 in a clinical Providencia rettgeri strain through the formation of a fusion plasmid

Identification of a novel Providencia species showing multi-drug-resistant in three patients with hospital-acquired infection

Hepatic failure with Providencia rettgeri infection: case report

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Emergence and Evolution of Unique Plasmids Harboring bla IMP-70 and bla CTX-M-253 in Multidrug-Resistant Providencia rettgeri

Cited by 3 publications

References 24 publications

Conjugation of plasmid harboring blaNDM-1 in a clinical Providencia rettgeri strain through the formation of a fusion plasmid

Conjugation of plasmid harboring blaNDM-1 in a clinical Providencia rettgeri strain through the formation of a fusion plasmid

Identification of a novel Providencia species showing multi-drug-resistant in three patients with hospital-acquired infection

Hepatic failure with Providencia rettgeri infection: case report

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Emergence and Evolution of Unique Plasmids Harboring bla _IMP-70 and bla _CTX-M-253 in Multidrug-Resistant Providencia rettgeri