Emergence and continued circulation of dengue‐2 (genotype IV) virus strains in northern India

Dash, Paban Kumar; Parida, Man Mohan; Saxena, Parag; Kumar, Manoj; Rai, Arvind; Pasha, Syed Tazeen; Jana, Asha Mukul

doi:10.1002/jmv.20166

Cited by 67 publications

(70 citation statements)

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“…Still, we have carried out the molecular epidemiology and genotyping study of the detected DENV-2 and DENV-3 viruses, during 2006 and 2007 circulation seasons. We studied the sequence of these dengue viruses, directly from patient serum sample, as recently advocated by several researchers [5,25]. Various genomic regions of dengue viruses have been selected [27].…”

Section: Discussionmentioning

confidence: 99%

Molecular surveillance of dengue virus in Bahia State, Brazil

Melo¹,

Domingo²,

Santos³

et al. 2012

AJMB

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Dengue is an important emerging viruses, posing a threat to one-third of the global human population. In 2002, the introduction of DENV-3 in the state of Bahia produced massive epidemic (about 35,000 cases detected) and the first cases of dengue hemorrhagic fever. To understand the nature of the virus circulating at Bahia, E/NS1 sequence was determined for 31 DENV viruses isolated in Bahia during the 2006 and 2007 transmission season, from patients presenting with different degrees of severity. The carboxi-terminal region of the E gene (220 nt) of 31 viruses, isolated from dengue patients with clinical diagnosis of dengue infection were used to determine the genetic variability of dengue 2 (DENV-2) and dengue 3 (DENV-3). Sequence data were used in phylogenetic comparisons with global samples of DENV-2 and DENV-3. DENV-2 sample grouped in the South East Indian genotype, while DENV-3 samples were grouped within Indian genotype. This study is the first report on Bahia isolates during two transmission seasons. Our data confirms reports from other parts of Brazil and different countries showing the DENV-3 (genotype III) strains circulating in the Americas are closely related, and cluster within the genotype that has been associated with DHF epidemics in different continents.

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Section: Discussionmentioning

confidence: 99%

Molecular surveillance of dengue virus in Bahia State, Brazil

Melo¹,

Domingo²,

Santos³

et al. 2012

AJMB

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“…All four serotypes of dengue cause similar type of infection, but DENV-2 and DENV-3 are more often associated with severe and fatal DHF. DENV-2 and DENV-3 are the most frequently found genotypes in South East Asia [14,15]. In Pakistan DF has caused many outbreaks from 1994 to 2011 [16].…”

Section: Introductionmentioning

confidence: 99%

Serotyping of Dengue Virus from Deadly Outbreaks of Pakistan

Waqar¹

2016

JHVRV

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Dengue Dengue virus (DENV) belonging to the family Flaviviridae consists of 4 serotypes (DEN-1, 2, 3 & 4) causing severe illnesses like dengue fever (DF), Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS). Dengue fever has become an emerging disease in Pakistan during the past 5-7 years with an increase in the number of cases every year since 2006. The current study aimed at identifying the serotypes of dengue virus involved in 2011 outbreak of Pakistan. Samples were collected from all provinces of Pakistan. Patients were confirmed in the hospital by antibody captured Enzyme linked immunosorbent assay (ELISA). These ELISA positive serum samples were inoculated on HeLa cell line to raise the viral titer of dengue virus and then RT-PCR was done for serotyping of dengue virus. Initially D1, D2 primers were tried for serotyping of dengue virus but poor results were obtained.Then serotype specific primers were designed having equal fragment length targeting NS1 gene. This approach was successful in serotyping of dengue virus from Pakistani population. On Real time RT-PCR, Dengue virus (DENV) was detected in 43.33% (n=26) patients out of 60 analyzed samples. Among 26 positive cases, 76.9% (n=20) were children and 23.1% (n=6) were adults and 46.1% (n=12) were male and 53.8 % (n=14) were females. ELISA results of 26 PCR positive cases show that 53.8% (n=14) were positive for IgM, 15.4 % (n=4) were positive for IgG and 30.8 % (n=8) were positive for both IgG and IgM. In this study DEN-2 was detected in 13.3% (n=8) patients, DEN-3 was detected in 16.67% (n=10) patients and DEN-2 & DEN-3 coinfection was detected in 13.3% (n=8) patients. We did not detected DEN-1 & DEN-4 in 2011 outbreak of Pakistan in 60 analyzed samples. By this study we can conclude that DEN-2 and DEN-3 were the main serotypes involved in 2011 outbreaks in Pakistan.

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“…Genotypes don't only differ in their geographical distribution, but also in their virulence and potential to cause large epidemics. [8,10] Various studies have also suggested the role of small genomic changes, leading to the lineages with greater fitness, epidemic potential and possibly the greater virulence of strains [11].The present study was carried out to understand the molecular epidemiology and evolutionary dynamics of DENV-2, circulated in Delhi during the post-monsoon period of 2013 and 2014.…”

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confidence: 97%

“…Nearly after a decade, DENV-2 reemerged as predominant serotype during the outbreak in 2013 [6]. The emergence of DENV-2 is an alarming signal for public health, as this serotype has been associated with major DF and DHF outbreaks [2,[6][7][8]. Based on genomic Electronic supplementary material The online version of this article…”

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Molecular epidemiology and evolutionary analysis of dengue virus type 2, circulating in Delhi, India

et al. 2016

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Dengue virus type 2 (DENV-2) has been associated with severe dengue outbreaks in many countries including India. Its predominance was recorded nearly after a decade in the capital city, Delhi in 2013. The present study characterizes DENV-2 circulated during 2013-2014. Analysis based on envelope (E) gene showed the presence of two clades (I and II) of DENV-2, within the Cosmopolitan genotype. Analysis of time of most recent common ancestor revealed the existence of clade I for more than a decade (95 % HPD 13-16 years) however, clade II showed comparatively recent emergence (95 % HPD 5-13 years). Presence of different clades is of high significance as this may result in increased virus transmission and major outbreaks. Further, the presence of a unique amino acid substitution, Q325H was also observed in an isolate; 14/D2/Del/2013 (KT717981). This substitution falls in immune epitope (epitope id: 150268) and may have important role in host immune response.Keywords Dengue Á Molecular epidemiology Á Envelope Á Evolutionary analysis Dengue fever is a mosquito borne infection caused by four antigenically-related serotypes of dengue virus (DENV-1, 2, 3 and 4). During the past decades dengue fever (DF) emerged as a major public health problem of global scale. Nearly 40 % of the world population is living at the risk of dengue infection. According to World Health Organization (available at http://www.searo.who.int/entity/vector_borne _tropical_diseases/data/data_factsheet/en/) it is the major cause of illness and deaths in tropical and subtropical regions, where Aedes mosquito population is high during rainy season. Dengue virus (DENV) belongs to the family Flaviviridae (genus; Flavivirus). Its genome consists of single stranded, positive sense RNA with approximately 11,000 nucleotides. A single open reading frame consists of three structural (capsid; C, premembrane/membrane; prM/ M and envelope; E) and seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) flanked by nontranslated regions (5 0 UTR and 3 0 UTR) [1].Dengue is endemic in several parts of India. Many outbreaks have been reported from different parts of the country. During last two decades, Delhi, the national capital has emerged as one of the ''badly hit'' city by dengue. The city witnessed its first major dengue hemorrhagic fever (DHF) outbreak in 1996 [2]. Dengue virus type 2 (DENV-2) was confirmed as main etiological agent for this outbreak. During another outbreak in 2003, co-circulation of DENV-2 and DENV-3 was observed [3]. However, in the following years a downward shift in predominance of DENV-2 was recorded. The outbreaks occurred in 2006 and 2010 were predominated by DENV-3 and DENV-1 respectively [4,5]. Nearly after a decade, DENV-2 reemerged as predominant serotype during the outbreak in 2013 [6]. The emergence of DENV-2 is an alarming signal for public health, as this serotype has been associated with major DF and DHF outbreaks [2,[6][7][8]. Based on genomic Electronic supplementary material The online version of this a...

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Emergence and continued circulation of dengue‐2 (genotype IV) virus strains in northern India

Cited by 67 publications

References 23 publications

Molecular surveillance of dengue virus in Bahia State, Brazil

Molecular surveillance of dengue virus in Bahia State, Brazil

Serotyping of Dengue Virus from Deadly Outbreaks of Pakistan

Molecular epidemiology and evolutionary analysis of dengue virus type 2, circulating in Delhi, India

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