Emergence and Characterization of a Novel IncP-6 Plasmid Harboring blaKPC–2 and qnrS2 Genes in Aeromonas taiwanensis Isolates

Hu, Xinjun; Shang, Yibing; Xu, Hao; Guo, Lan-Ping; Liang, Yile; Kang, Yixin; Song, Li; Sun, Jifeng; Feng, Yue; Mao, Yimin; Zheng, Beiwen

doi:10.3389/fmicb.2019.02132

Cited by 20 publications

(24 citation statements)

References 34 publications

(41 reference statements)

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“…The most abundant replicons were repUS2, IncP(6), and IncQ2, in this order. RepUS2 plasmids are narrow-range plasmids, present in Gram-positive bacteria, and have been recently found to be prevalent in gut microbiome 40 , while IncP(6) and IncQ2 are broad-range conjugative plasmids that contribute to the acquisition of multidrug resistance in a variety of Gram-negative bacteria [41][42][43][44][45] . (Fig.…”

Section: Antibiotic Resistance Genesmentioning

confidence: 99%

Metagenomic analysis of an urban resistome before and after wastewater treatment

Lira¹,

Vaz‐Moreira

Tamames³

et al. 2020

Sci Rep

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Determining the effect of wastewater treatment in water resistome is a topic of interest for water quality, mainly under re-use and one-Health perspectives. the resistome, the plasmidome, and the bacterial community composition of samples from influents and treated effluents from a wastewater treatment plant located in northern portugal were studied using metagenomic techniques. Wastewater treatment contributed to reduce the abundance of resistance genes and of plasmid replicons, coinciding with a decline in the number of intrinsic resistance genes from Enterobacteriaceae, as well as with a reduction in the relative abundance of Firmicutes and Proteobacteria after treatment. these taxons comprise bacterial pathogens, including those belonging to the eSKApe group, which encompasses bacteria with the highest risk of acquiring antibiotic resistance, being the most relevant hosts of resistance genes acquired through horizontal gene transfer. our results support that wastewater treatment efficiently removes the hosts of antibiotic resistance genes and, consequently, the harboured antibiotic resistance genes. principal component analysis indicates that the resistome and the bacterial composition clustered together in influent samples, while did not cluster in final effluent samples. our results suggest that wastewater treatment mitigates the environmental dissemination of urban resistome, through the removal of the hosts harbouring mobile resistance genes. Most studies on antibiotic resistance have concentrated their efforts on human pathogens and in human-linked environments (hospitals, human hosts,…). However, the full understanding of the origin, evolution, spread, and maintenance of antibiotic resistance (AR) requires an integrative approach in which all ecosystems that may contribute to such evolution are studied; a One Health approach 1,2. One Heath is a concept derived from the idea proposed by Rudolph Virchow in the 19th Century, stating that "between animal and human medicine there are no dividing lines-nor should there be". The idea behind is that, at least in the case of infectious diseases, different ecosystems, not just the human host, may contribute to the spread of the infective agents. This is particularly relevant in the case of AR, since it is generally accepted that antibiotic resistance genes (ARGs), currently present in human pathogens, are originated in environmental organisms and, therefore, natural ecosystems can be reservoirs and participate in the spread of AR 3-9. Consequently, it has been proposed that AR is the quintessential One Health issue 2 , and that studies, integrating all habitats potentially involved in the evolution and spread of AR are needed 1,10,11. Indeed, seminal work showed that animal farms could be a reservoir of AR 12. As a consequence, the use of antibiotics in farming, for non-therapeutic or prophylactic procedures (as animal fattening), has been banned in different countries 13,14. More recently, non-clinical environments (natural and man-made ecosystems) have been pro...

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Section: Antibiotic Resistance Genesmentioning

confidence: 99%

Metagenomic analysis of an urban resistome before and after wastewater treatment

Lira¹,

Vaz‐Moreira

Tamames³

et al. 2020

Sci Rep

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“…Autochtonous bacteria can also acquire new CEG. Environmental aquatic bacterial genera, which acquired clinical relevant CEG from contaminated water have been reported in many previous studies [ 11 , 13 , 15 , 16 , 39 , 40 , 94 , 99 ]. As autochtonous bacteria are adapted to persist in the aquatic environment, they can constitute a perennial environmental reservoir for CEG.…”

Section: Presence Of Cpb In Aquatic Environments: a Simply Remnantmentioning

confidence: 98%

“…A Chinese study identified an IncP-6 plasmid carrying bla KPC-2 gene in three isolates of Aeromonas taiwanensis isolated from sediments of an urban river. Interestingly the bla KPC-2 gene was inserted in a Tn 3 transposon as previously reported in clinical Enterobacterales in China and in France [ 40 ]. bla KPC genes have also be found to be carried by a conjugative plasmid in a K. pneumoniae strain isolated from urban lake water [ 41 ] and in Citrobacter freundii and A. caviae strains isolated from an urban river close to a WWTP [ 42 ].…”

Section: Carbapenemases: a Clinical Success Storymentioning

confidence: 99%

“…A Chinese study identified of an IncP-6 plasmid encoding bla KPC-2 gene in three A. taiwanensis strains isolated from sediments of an urban water. Interestingly, the bla KPC-2 gene was inserted in a transposon Tn 3 , which had already been reported in Enterobacterales in China and in France [ 40 ]. Others studies noticed environmental Aeromonas spp.…”

Section: The Bacterial Shuttle Concept: When Hydric Autochtonous Bmentioning

confidence: 99%

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The Hydric Environment: A Hub for Clinically Relevant Carbapenemase Encoding Genes

2020

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Carbapenems are β-lactams antimicrobials presenting a broad activity spectrum and are considered as last-resort antibiotic. Since the 2000s, carbapenemase producing Enterobacterales (CPE) have emerged and are been quickly globally spreading. The global dissemination of carbapenemase encoding genes (CEG) within clinical relevant bacteria is attributed in part to its location onto mobile genetic elements. During the last decade, carbapenemase producing bacteria have been isolated from non-human sources including the aquatic environment. Aquatic ecosystems are particularly impacted by anthropic activities, which conduce to a bidirectional exchange between aquatic environments and human beings and therefore the aquatic environment may constitute a hub for CPE and CEG. More recently, the isolation of autochtonous aquatic bacteria carrying acquired CEG have been reported and suggest that CEG exchange by horizontal gene transfer occurred between allochtonous and autochtonous bacteria. Hence, aquatic environment plays a central role in persistence, dissemination and emergence of CEG both within environmental ecosystem and human beings, and deserves to be studied with particular attention.

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“…The plasmid-mediated qnrS2 genes have been identified mainly in representatives of Enterobacteriaceae, Shewanella sp., Vibrionaceae, and Aeromonas spp. [47][48][49][50][51]. While the analyzed IncQ-3 plasmids were mostly isolated from Aeromonas spp.…”

Section: Incq-3 Plasmids As a Reservoir Of Antibiotic Resistance Genementioning

confidence: 99%

Molecular Characterization and Comparative Genomics of IncQ-3 Plasmids Conferring Resistance to Various Antibiotics Isolated from a Wastewater Treatment Plant in Warsaw (Poland)

et al. 2020

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As small, mobilizable replicons with a broad host range, IncQ plasmids are widely distributed among clinical and environmental bacteria. They carry antibiotic resistance genes, and it has been shown that they confer resistance to β-lactams, fluoroquinolones, aminoglycosides, trimethoprim, sulphonamides, and tetracycline. The previously proposed classification system divides the plasmid group into four subgroups, i.e., IncQ-1, IncQ-2, IncQ-3, and IncQ-4. The last two subgroups have been poorly described so far. The aim of this study was to analyze five newly identified IncQ-3 plasmids isolated from a wastewater treatment plant in Poland and to compare them with all known plasmids belonging to the IncQ-3 subgroup whose sequences were retrieved from the NCBI database. The complete nucleotide sequences of the novel plasmids were annotated and bioinformatic analyses were performed, including identification of core genes and auxiliary genetic load. Furthermore, functional experiments testing plasmid mobility were carried out. Phylogenetic analysis based on three core genes (repA, mobA/repB, and mobC) revealed the presence of three main clusters of IncQ-3 replicons. Apart from having a highly conserved core, the analyzed IncQ-3 plasmids were vectors of antibiotic resistance genes, including (I) the qnrS2 gene that encodes fluoroquinolone resistance and (II) β-lactam, trimethoprim, and aminoglycoside resistance genes within integron cassettes.

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Emergence and Characterization of a Novel IncP-6 Plasmid Harboring blaKPC–2 and qnrS2 Genes in Aeromonas taiwanensis Isolates

Cited by 20 publications

References 34 publications

Metagenomic analysis of an urban resistome before and after wastewater treatment

Metagenomic analysis of an urban resistome before and after wastewater treatment

The Hydric Environment: A Hub for Clinically Relevant Carbapenemase Encoding Genes

Molecular Characterization and Comparative Genomics of IncQ-3 Plasmids Conferring Resistance to Various Antibiotics Isolated from a Wastewater Treatment Plant in Warsaw (Poland)

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