Emergence and characterization of a putative novel human adenovirus recombinant HAdV-C104 causing pneumonia in Southern China

Ji, Tianxing; Li, Ling; Li, Wenrui; Zheng, Xuehua; Ye, Xianmiao; Chen, Hongliang; Zhou, Qiang; Jia, Hongyun; Chen, Bo; Lin, Zhen; Chen, Haoyu; Huang, Shunlin; Seto, Donald; Chen, Ling; Feng, Liqiang

doi:10.1093/ve/veab018

Cited by 13 publications

(11 citation statements)

References 22 publications

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“…By decreasing the viral load of the HAdV-5 population, a type of HAdV with global circulation and high seroprevalence in adult patients (73%) (244,245). This could create new recombinants in previously vaccinated immunosuppressed patients (246), who, by presenting concomitant exposures of different adenoviral types, could potentially generate more aggressive recombinants. If the environmental and host conditions are adequate, recombinant pandemic precursor strains could be generated.…”

Section: Future Projections Of Hadv Study In the 21st Centurymentioning

confidence: 99%

An Old Acquaintance. Could Adenovirus be our Next Pandemic Threat?

Saint-Pierre¹,

Conei²,

Lizama³

et al. 2022

Preprint

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Human adenoviruses are one of the most important pathogens detected in acute respiratory diseases in pediatrics and immunocompromised patients. In 1953, Wallace Rowe described it for the first time in oropharyngeal lymphatic tissue. To date, more than 85 types of HAdV have been described, with different cellular tropisms. They can cause respiratory and gastrointestinal symptoms, even urinary tract inflammation, although most infections are asymptomatic. However, there is a population at risk that can develop serious and even lethal conditions. These viruses have a double-stranded DNA genome, 25-48 kbp, 90 nm in diameter, without a mantle, stable in the environment, and resistant to fat-soluble detergents. Currently the diagnosis is made with lateral flow immunochromatography or molecular biology through a polymerase chain reaction. The objective of this review is to recognize the variability of HAdV, the pandemic potential that a recombinant could present between HAdV-3 and 7 viral types, known to produce aggressive outbreaks in health facilities. The review determined the characteristics of HAdV, from the infection to treatment, vaccine development and the evaluation of the social determinants of health associated with HAdV, guiding the necessary measures for future sanitary control, and preventing disasters such as the SARS-CoV-2 pandemic.

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Section: Future Projections Of Hadv Study In the 21st Centurymentioning

confidence: 99%

An Old Acquaintance. Could Adenovirus be our Next Pandemic Threat?

Saint-Pierre¹,

Conei²,

Lizama³

et al. 2022

Preprint

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“…Adenoviruses have been isolated from many mammals, such as humans, monkeys, bovines, and mice. Human adenoviruses contain more than 100 types ( , accessed on 5 February 2022), which belong to seven species (HAdV-A to -G) [ 1 , 2 ]. HAdV-5, as a prototype of HAdV-C, has been intensively studied and reconstructed as gene transfer vectors.…”

Section: Introductionmentioning

confidence: 99%

Restriction-Assembly: A Solution to Construct Novel Adenovirus Vector

Guo

Sun

Chen

et al. 2022

Viruses

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Gene therapy and vaccine development need more novel adenovirus vectors. Here, we attempt to provide strategies to construct adenovirus vectors based on restriction-assembly for researchers with little experience in this field. Restriction-assembly is a combined method of restriction digestion and Gibson assembly, by which the major part of the obtained plasmid comes from digested DNA fragments instead of PCR products. We demonstrated the capability of restriction-assembly in manipulating the genome of simian adenovirus 1 (SAdV-1) in this study. A PCR product of the plasmid backbone was combined with SAdV-1 genomic DNA to construct an infectious clone, plasmid pKSAV1, by Gibson assembly. Restriction-assembly was performed repeatedly in the steps of intermediate plasmid isolation, modification, and restoration. The generated adenoviral plasmid was linearized by restriction enzyme digestion and transfected into packaging 293 cells to rescue E3-deleted replication-competent SAdV1XE3-CGA virus. Interestingly, SAdV1XE3-CGA could propagate in human chronic myelogenous leukemia K562 cells. The E1 region was similarly modified to generate E1/E3-deleted replication-defective virus SAdV1-EG. SAdV1-EG had a moderate gene transfer ability to adherent mammalian cells, and it could efficiently transduce suspension cells when compared with the human adenovirus 5 control vector. Restriction-assembly is easy to use and can be performed without special experimental materials and instruments. It is highly effective with verifiable outcomes at each step. More importantly, restriction-assembly makes the established vector system modifiable, upgradable and under sustainable development, and it can serve as the instructive method or strategy for the synthetic biology of adenoviruses.

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“…Human adenoviruses (HAdVs) are non-enveloped, double-stranded DNA viruses of the family Adenoviridae . More than 100 genotypes of HAdVs have been identified, which are classified into seven species (A–G) ( Ismail et al, 2018 ; Ji et al, 2021 ). HAdVs are associated with a broad spectrum of clinical diseases, such as acute respiratory illness (ARI), conjunctivitis, gastrointestinal infections, and obesity ( Sandkovsky et al, 2014 ; Radke and Cook, 2018 ; Chen et al, 2020 ; Ji et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…More than 100 genotypes of HAdVs have been identified, which are classified into seven species (A–G) ( Ismail et al, 2018 ; Ji et al, 2021 ). HAdVs are associated with a broad spectrum of clinical diseases, such as acute respiratory illness (ARI), conjunctivitis, gastrointestinal infections, and obesity ( Sandkovsky et al, 2014 ; Radke and Cook, 2018 ; Chen et al, 2020 ; Ji et al, 2021 ). Members of species B are known to cause human diseases, with HAdV types 3, 7, 14, and 55 being the most common causes of respiratory disease outbreaks ( Prusinkiewicz and Mymryk, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Human Adenovirus Subtype 21a Isolates From Children With Severe Lower Respiratory Illness in China

et al. 2022

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Human adenovirus type 21 (HAdV-21) is an important pathogen associated with acute respiratory infection (ARI), but it was rarely reported and characterized so far. In this study, 151 of 1,704 (8.9%) pediatric patients (≤14 years old) hospitalized with ARI in Guangzhou, China in 2019 were positive for HAdV which was the third most frequently detected pathogen. Two HAdV-21-positive patients presented with severe lower respiratory illness and had similar initial symptoms at onset of illness. Then two HAdV-21 strains were isolated and characterized. The two HAdV-21 strains were sequenced and classified as subtype 21a with genomes closely related to strain BB/201903 found in Bengbu, China in March 2019. Phylogenetic analysis for whole genome and major antigen proteins of global HAdV-21 strains showed that HAdV-21 could be classified into two branches, branch 1 including genotype 21p, branch 2 including all other strains dividing into genotype 21a and 21b. There was no significant difference in the plaque size, or the replication curves between the two HAdV-21a strains and the prototype strain HAdV-21p AV-1645. However, there were five highly variable regions (HVR1, HVR3, HVR4, HVR5, and HVR7) in the hexon protein that varied between two branches. Mice immunized with one branch strain showed 2–4-fold lower neutralizing antibody titers against another branch strain. In summary, this study firstly reported two HAdV-21a infections of children in China, characterized two isolates of HAdV-21a associated with severe lower respiratory illness; our results could be important for understanding the HAdV-21 epidemiology and pathogenic, and for developing HAdV-21 vaccine and drug.

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Emergence and characterization of a putative novel human adenovirus recombinant HAdV-C104 causing pneumonia in Southern China

Cited by 13 publications

References 22 publications

An Old Acquaintance. Could Adenovirus be our Next Pandemic Threat?

An Old Acquaintance. Could Adenovirus be our Next Pandemic Threat?

Restriction-Assembly: A Solution to Construct Novel Adenovirus Vector

Human Adenovirus Subtype 21a Isolates From Children With Severe Lower Respiratory Illness in China

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