Emended description of Campylobacter sputorum and revision of its infrasubspecific (biovar) divisions, including C. sputorum biovar paraureolyticus, a urease-producing variant from cattle and humans

Abstract

A polyphasic taxonomic study of 15 bovine and human strains assigned to the catalase-negative, urease-positive campylobacter (CNUPC) group identified these bacteria as a novel, ureolytic biovar of Campylobacter sputorum for which w e propose the name C. sputorum bv. paraureolyticus : suitable reference strains are LMG 11764 (human isolate) and LMG 17590 (= CCUG 37579, bovine isolate). The present study confirmed previous findings showing that the salient biochemical tests used to differentiate C. sputorum bv. sputorum from C. sputorum bv. bubulus are not reproducible; and that the absolute validity of source-specific biovars of the species is questionable. A correlation between the results of numerical analysis of protein profiles and the reaction of strains in certain enzyme tests was, however, noted. Therefore, it is proposed that the infrasubspecific (biovar) divisions of C. sputorum should be revised to include bv. sputorum for catalase-negative strains; bv. fecalis for catalase-positive strains; and bv. paraureolyticus for urease-positive strains. Strains classified previously as bv. bubulus should be reclassified as bv. sputorum. The species description of C. sputorum is revised accordingly.

Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences

“…Human isolate 6871, tentatively identified as C. coli, was further characterized by hippuricase PCR (21); 16S rRNA gene sequencing with primers 27f, 518r, 536f, 1054r, 1073f, and 1492r (10,26); 23S rRNA gene PCR-RFLP analysis (11); and dot blot hybridization (12). Hybridization was performed with genomic DNA isolated from C. jejuni reference strain NCTC 11168 and labeled with digoxigenin-11-dUTP (DIG-High Prime; Roche Diagnostics GmbH, Mannheim, Germany) as the probe.…”

Campylobacter

“…Rapid PCR-biprobe identification scheme based on the realtime PCR was developed for the Campylobacter taxa pathogenic for humans, including C. lanienae [82]. This new organism has also been isolated from the faeces of six healthy pigs in Japan [148] and from the faeces of bovine and beef cattle, in the beef cattle, C. lanienae was the most frequently detected species (49%), in Canada [62,63,115,118]. In addition, an intervening sequence of 226 bp in the 16S rDNA was found in four isolates of the six of C. lanienae in Japan [148].…”

“…In 1998, On et al first identified the 15 strains isolated from faeces of 14 cattle in United Kingdom and one human diarrhoea in Canada among 44 catalase negative and urease-positive Campylobacter group as a new C. sputorum biovar paraureolyticus, by a phylogenetic study based on phenotypic characterization, numerical analysis of whole-cell protein profiles, DNA-DNA hybridization and sequence analysis of 16S rDNA [115]. They demonstrated the clonality of C. sputorum bv.…”

Campylobacter geochelonis sp. nov. isolated from the western Hermann's tortoise (Testudo hermanni hermanni)

“…peloridis; 25, C. rectus; 26, C. showae; 27, C. sputorum; 28, C. subantarcticus; 29, C. upsaliensis; 30, C. ureolyticus; 31, C. volucris. Data for reference taxa were taken from the original descriptions (Tanner et al, 1981;Roop II et al, 1985;Vandamme et al, 1995Vandamme et al, , 2010On et al, 1998;Lawson et al, 2001;Debruyne et al, 2009Debruyne et al, , 2010aFitzgerald et al, 2014;Koziel et al, 2014) and from Inglis et al (2007), Rossi et al (2009) and Gilbert et al (2015). +, 90-100 %; (+), 75-89 %; V, 26-74 %; (À), 11-25 %;…”