Embryotoxicity in vitro with extract of Indigofera suffruticosa leaves

Leite, Sônia Pereira; Medeiros, Paloma Lys de; Silva, Eliete Cavalcanti da; Maia, Maria Bernadete de Souza; Lima, Vera Lúcia de Menezes; Saul, Daniel Eduardo

doi:10.1016/j.reprotox.2004.04.004

Cited by 26 publications

(27 citation statements)

References 21 publications

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“…The results of the present study suggest that morphological alterations of the hepatocyte nuclei after subchronic treatment with aqueous extracts of I. suffruticosa leaves may be due to their interference with DNA synthesis. The same mechanisms of aqueous extract of I. suffruticosa leaves by infusion may be involved in the embryo development in mice (Leite et al, 2004). Biological activities of the compounds detected in the aqueous extracts of leaves of I. suffruticosa by infusion and maceration could be linked to antitumor activity found by Vieira et al (2007b) and Silva et al (2010).…”

Section: Discussionmentioning

confidence: 78%

“…during seven consecutive days. The dose of the compound was based on the LD50 (Leite et al, 2004). On the eighth day, the mice were sacrificed by cervical dislocation and the livers were removed for histopathological and histomorphometric studies.…”

Section: Methodsmentioning

confidence: 99%

“…Some pharmacological studies using aqueous extract of I. suffruticosa have shown embryotoxic (Leite et al, 2004) antimicrobial (Leite et al, 2006), anti-inflammatory (Leite et al, 2003), antitumor and cytotoxic activities (Vieira et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Indigofera suffruticosa Mill (Fabaceae): Hepatic Responses in Mice Bearing Sarcoma 180

et al. 2014

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SUMMARY:Indigofera suffruticosa is a plant generally used to treat infectious and inflammatory processes. This work aims to evaluate the histopathological changes in the liver tissue of mice with Sarcoma 180 after subchronic treatment with aqueous extract obtained by infusion and maceration of Indigofera suffruticosa leaves. Male mice were divided into four groups of six animals: G1, G2 and G3 patients with Sarcoma 180 and Sarcoma 180 G4 without sarcoma. G1 and G2 were treated with infusion mashing respectively (50 mg/kg ip); G3 and G4 controls received saline (15 ml/kg ip). The histopathological and morphometric analysis of liver tissue after subchronic treatment with aqueous extracts by infusion and maceration of the groups G1, G2 and G4 were similar and showed no degraded areas or leukocyte infiltration compared to G3, which shows a marked destruction of liver architecture. The results showed that after subchronic treatment with the aqueous extract of leaves Indigofera Suffruticosa obtained by infusion and maceration, the hepatic architecture was preserved, suggesting its use as an alternative hepatoprotective agent.

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Section: Discussionmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

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Indigofera suffruticosa Mill (Fabaceae): Hepatic Responses in Mice Bearing Sarcoma 180

et al. 2014

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“…This species is typical of the "cerrado" or Brazilian savannah, and has been used to treat gastrointestinal disorders such as gastrointestinal pain (Roig 1988). This species is known to be a rich source of flavonoids, alkaloids, steroids, proteins and carbohydrates (Leite et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Antiulcerogenic activity of Indigofera truxillensis Kunth

et al. 2006

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Cola-Miranda, M; Barbastefano, V.; Hiruma-Lima, C.A.; Calvo, T.R.; Vilegas, W. and Brito, A.R.M.S. Antiulcerogenic activity of Indigofera truxillensis Kunth. Biota Neotrop. Sep/Dec 2006 vol. 6, no. 3 http://www.biotaneotropica.org.br/v6n3/ pt/abstract?article+bn01006032006 ISSN 1676-0611The genus Indigofera (Fabaceae) is used in folk medicine to treat gastrointestinal pain. In this study, we investigated the antiulcerogenic properties of Indigofera truxillensis Kunth. Oral administration of MeOH extract did not produce any signals of acute toxicity. The antiulcerogenic activity was assessed in different models of acute gastric ulcers (100% ethanol, piroxicam 30 mg.kg -1 , hypothermic restraint stress and pylorus ligature) in mice and rats. The animals were treated with the drugs lanzoprazole (30 mg.kg -1 ) or cimetidine (100 mg.kg -1 ) as positive controls depending on the performed model. In another experiment with ethanol-induced ulcers in rats, N-ethylmaleimide (NEM), a sulfhydryl group blocker, was also used. The MeOH extract, at doses of 250, 500 and 1000 mg.kg -1 , inhibited the gastric lesions in all experiments: a) by 62%, 69% and 32%, respectively, in piroxicam-induced lesions, b) by 43%, 71% and 98%, in ethanol-induced lesions, c) by 69%, 64 and 89%, in hypothermic-restraint stress-induced lesions, d) by 73%, 82% and 84%, in pylorus ligature lesions. Significant changes in the total gastric acid levels were also found after intraduodenal administration of the MeOH extract in the ligated pylorus model. Pre treatment with NEM reduced partially the antiulcerogenic activity of the MeOH extract in ethanolinduced gastric lesions. This result indicates an increase in the levels of non-protein sulfhydryl groups by MeOH extract in the gastric mucosa. These results indicate that the MeOH extract has antisecretory and citoprotective effects that may be related to the presence of flavonoids detected by phytochemical analysis. O gênero Indigofera (Fabaceae) é utilizado na medicina tradicional para distúrbios gastrintestinais. Em nosso trabalho foi investigada a propriedade antiulcerogênica da Indigofera truxillensis Kunth. A administração oral do extrato metanólico (MeOH) não produziu efeitos tóxicos. A atividade antiulcerogênica foi avaliada em diferentes modelos agudos de úlcera gástrica (etanol 100%, piroxicam 30 mg.kg -1 , estresse por retenção e frio e ligadura do piloro) em camundongos e ratos. Os animais foram tratados com lansoprazol (30 mg.kg -1 ) ou cimetidina (100 mg.kg -1 ), que foram utilizados como controle positivo dependendo do modelo testado. Em outro experimento com úlcera induzida por etanol em ratos, N-etilmaleimida (NEM), um bloqueador dos compostos sulfidríla, também foi utilizado. O extrato metanólico, nas doses de 250, 500 e 1000 mg.kg -1 , inibiu significativamente as lesões gástricas em todos os experimentos: a) 62%, 69% e 32%, respectivamente, nas lesões gástricas induzidas por piroxicam, b) 43%, 71% e 98%, nas lesões gástricas induzidas por etanol, c) 69%, 64% e 89%, nas lesões ...

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“…Recently, antitumoral and antimicrobial activities and mice embryotoxic effects have been tested with extract of leaves of I. suffruticosa [10, 12–14]. …”

Section: Introductionmentioning

confidence: 99%

Oviposition and Embryotoxicity ofIndigofera suffruticosaon Early Development ofAedes aegypti(Diptera: Culicidae)

Vieira

Leite

Lima

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Aqueous extract of Indigofera suffruticosa leaves obtained by infusion was used to evaluate the oviposition, its effect on development of eggs and larvae, and morphological changes in larvae of Aedes aegypti. The bioassays were carried out with aqueous extract in different concentrations on eggs, larvae, and female mosquitoes, and the morphological changes were observed in midgut of larvae. The extract showed repellent activity on A. aegypti mosquitoes, reducing significantly the egg laying by females with control substrate (343 (185–406)) compared with the treated substrate (88 (13–210)). No eclosion of A. aegypti eggs at different concentrations studied was observed. The controleclodedin 35%. At concentration of 250 μg/mL, 93.3% of larvae remained in the second instar of development and at concentrations of 500, 750, and 1000 μg/mL the inhibitory effect was lower with percentages of 20%, 53.3%, and 46.6%, respectively. Morphological changes like disruption on the peritrophic envelope (PE), discontinued underlying epithelium, increased gut lumen, and segments with hypertrophic aspects were observed in anterior region of medium midgut of larvae of A. aegypti. The results showed repellent activity, specific embryotoxicity, and general growth retardation in A. aegypti by medium containing aqueous extract of I. suffruticosa leaves.

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Embryotoxicity in vitro with extract of Indigofera suffruticosa leaves

Cited by 26 publications

References 21 publications

Indigofera suffruticosa Mill (Fabaceae): Hepatic Responses in Mice Bearing Sarcoma 180

Indigofera suffruticosa Mill (Fabaceae): Hepatic Responses in Mice Bearing Sarcoma 180

Antiulcerogenic activity of Indigofera truxillensis Kunth

Oviposition and Embryotoxicity ofIndigofera suffruticosaon Early Development ofAedes aegypti(Diptera: Culicidae)

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