Embryotoxic Effect of Cadmium Chloride on Rat Body

Shamelashvili, K. L.; Шаторна, В. Ф.

doi:10.29254/2077-4214-2021-1-159-147-150

Cited by 3 publications

(4 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When analyzing indicators of general embryotoxic mortality, pre-implantation mortality, and post-implantation mortality, we noticed that they were significantly lower in the experimental group compared to the control group (Table 2). The obtained experimental data indicate the absence of an embryotoxic effect, which was also confirmed by other authors in relation to medicinal leech substances (Shatorna et al, 2022;Grytsaj et al, 2022;Shamelashvili et al, 2021;Shatorna et al, 2020;Nefodova et al, 2018). It should also be noted that body weight and length of animals in the experimental group increased within the physiological norms for this age (p < 0.05).…”

Section: Control Experimentalsupporting

confidence: 89%

“…Subsequently, one of the groups, from both control and experimental female rats, was euthanized under light ether anesthesia by dislocation of the cervical vertebrae on the 20th day of pregnancy (15 animals in each group). In these female rats, abdominal cavity and uterine horns were dissected; the number of corpora lutea of pregnancy, and the number of live and dead fetuses were analyzed (Shatorna et al, 2022;Shamelashvili et al, 2021;Shatorna et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…The embryotoxic effect of the studied substances was evaluated according to the following indicators (Shatorna et al, 2022 ;Shamelashvili et al, 2021;Shatorna et al, 2020) where A is the number of living fetuses; B -the number of dead fetuses.…”

Section: Methodsmentioning

confidence: 99%

“…This especially concerns their use by pregnant women and children. Thereby, the study of the embryotoxic and teratogenic effects of substances is an important precondition for their practical use (Shatorna et al, 2022;Grytsaj et al, 2022;Shamelashvili et al, 2021;Shatorna et al, 2020;Nefodova et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The influence of the water-salt extract of the medicinal leech Hirudo verbana Carena, 1820 on the general course of embryogenesis in rats after intraperitoneal administration

Aminov¹

2023

Biol. Stud.

View full text Add to dashboard Cite

Background. The increase in infectious and non-infectious diseases occurrence during pregnancy prompts scientists to search for substances that can fight these ailments. This especially applies to natural substances that have virtually no side effects. These include substances obtained from medicinal leeches. These substances have a wide range of therapeutic effects; as to their practical use, all preclinical studies must be completed. This particularly refers to the presence of an embryotoxic effect. The aim of this work was to investigate the effect of the water-salt extract of the medicinal leech Hirudo verbana Carena, 1820 on the general course of embryogenesis in rats when administered intraperitoneally. Materials and Methods. The work was performed on female non-linear white rats. Two experimental groups of animals were injected intraperitoneally with a water-salt extract of the medicinal leech at a concentration of 5 μg/g of the animal, in a volume of 0.5 mL, three times before mating and three times during pregnancy every 3 days. Two control groups were injected intraperitoneally with physiological saline according to the same scheme as the experimental groups. Subsequently, one of the control and experimental groups underwent euthanasia, dissection of the abdominal cavity and uterine horns on the 20th day of pregnancy. We analyzed the total number of corpora lutea of pregnancy, the number of live and dead fetuses. Fetuses were weighed, examined for the presence of skin hematomas, mutilations, and external abnormalities in the development of internal organs. The other two groups of control and experimental animals were left until the offspring were born. Then the females of both groups were weighed, and their general condition was assessed. Newborn rats were examined, weight and body length were determined, mortality was recorded from the beginning of birth to 15 days, the presence of skin hematomas, mutilations, and external anomalies of the development of internal organs were checked. Results and discussion. The study established that the intraperitoneal injection of a water-salt extract of a medicinal leech before and during pregnancy in female rats does not cause any toxic effect during embryogenesis and at the early stages of post-embryonic ontogenesis. During the external examination of fetuses and offspring in both groups, no developmental anomalies were noted, all organs in both groups were of normal topography and size. The number of subcutaneous hematomas in the experimental group of fetuses compared to the control group decreased by an average of 50%, postnatal mortality decreased by an average of more than 3 times, as well as an increase in body weight in females, an increase in body weight and body length in the fetus and offspring in the early post-embryonic period ontogenesis (p <0.05). Conclusion. Intraperitoneal administration of a therapeutic dose of a water-salt extract of the medicinal leech to female rats before and during pregnancy, after every three days, does not have any toxic effect on the course of embryogenesis and later in early post-embryonic ontogenesis.

show abstract

Section: Control Experimentalsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%