Embryos of the zebrafish Danio rerio in studies of non-targeted effects of ionizing radiation

Choi, V.W.Y.; Yu, K.N.

doi:10.1016/j.canlet.2013.10.020

Cited by 27 publications

(21 citation statements)

References 218 publications

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“…The present paper reviewed the potential of the zebrafish, Danio rerio , as an in vivo model to assess the epigenetic effects of IR. Embryogenesis is a stage which has rapid changes in the epigenetic status and which is markedly radiosensitive, so zebrafish embryos are in particular ideal for studying epigenetic mechanisms and for evaluating responses to IR [30,253]. …”

Section: Discussionmentioning

confidence: 99%

“…These included the bystander effect, adaptive response, rescue effect, genomic instability, changed profiles of gene expression and of non-coding RNAs. Radiation-induced bystander effects (RIBEs) refer to biological effects which occurred in unirradiated cells/organisms after they were put in contact with the irradiated cells/organisms or immersed in the medium having previously been conditioned by the irradiated cells/organisms [9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31]. Adaptive response (AR) referred to mitigation of the biological effectiveness of a large challenging dose in cells/organisms through application of a small preceding priming dose [32], and the first in vitro study demonstrating AR was reported by Olivieri et al [33].…”

Section: Ionizing Radiation Targeted Effects and Non-targeted Effmentioning

confidence: 99%

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Zebrafish as an In Vivo Model to Assess Epigenetic Effects of Ionizing Radiation

Kong

Cheng

2016

IJMS

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Exposure to ionizing radiations (IRs) is ubiquitous in our environment and can be categorized into “targeted” effects and “non-targeted” effects. In addition to inducing deoxyribonucleic acid (DNA) damage, IR exposure leads to epigenetic alterations that do not alter DNA sequence. Using an appropriate model to study the biological effects of radiation is crucial to better understand IR responses as well as to develop new strategies to alleviate exposure to IR. Zebrafish, Danio rerio, is a scientific model organism that has yielded scientific advances in several fields and recent studies show the usefulness of this vertebrate model in radiation biology. This review briefly describes both “targeted” and “non-targeted” effects, describes the findings in radiation biology using zebrafish as a model and highlights the potential of zebrafish to assess the epigenetic effects of IR, including DNA methylation, histone modifications and miRNA expression. Other in vivo models are included to compare observations made with zebrafish, or to illustrate the feasibility of in vivo models when the use of zebrafish was unavailable. Finally, tools to study epigenetic modifications in zebrafish, including changes in genome-wide DNA methylation, histone modifications and miRNA expression, are also described in this review.

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Section: Discussionmentioning

confidence: 99%

Section: Ionizing Radiation Targeted Effects and Non-targeted Effmentioning

confidence: 99%

Zebrafish as an In Vivo Model to Assess Epigenetic Effects of Ionizing Radiation

Kong

Cheng

2016

IJMS

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“…These effects were assessed using the number of apoptotic events in the zebrafish embryos at 24 hpf revealed through AO staining, which was a common adopted biological endpoint for studying radiation-induced effects in zebrafish embryos [20]. …”

Section: Discussionmentioning

confidence: 99%

“…Zebrafish has become a popular vertebrate model in genetic, pharmacological and behavioral studies. In relation, zebrafish embryos have also been widely employed for examining biological effects of ionizing radiations [18,19,20,21,22,23]. The most important advantage of using zebrafish as an animal model in studying the biological effects is that zebrafish share considerable genetic sequence similarity with humans [24,25].…”

Section: Introductionmentioning

confidence: 99%

Exogenous Nitric Oxide Suppresses in Vivo X-ray-Induced Targeted and Non-Targeted Effects in Zebrafish Embryos

Kong

Yeung

Chan

et al. 2016

IJMS

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The present paper studied the X-ray-induced targeted effect in irradiated zebrafish embryos (Danio rerio), as well as a non-targeted effect in bystander naïve embryos partnered with irradiated embryos, and examined the influence of exogenous nitric oxide (NO) on these targeted and non-targeted effects. The exogenous NO was generated using an NO donor, S-nitroso-N-acetylpenicillamine (SNAP). The targeted and non-targeted effects, as well as the toxicity of the SNAP, were assessed using the number of apoptotic events in the zebrafish embryos at 24 h post fertilization (hpf) revealed through acridine orange (AO) staining. SNAP with concentrations of 20 and 100 µM were first confirmed to have no significant toxicity on zebrafish embryos. The targeted effect was mitigated in zebrafish embryos if they were pretreated with 100 µM SNAP prior to irradiation with an X-ray dose of 75 mGy but was not alleviated in zebrafish embryos if they were pretreated with 20 µM SNAP. On the other hand, the non-targeted effect was eliminated in the bystander naïve zebrafish embryos if they were pretreated with 20 or 100 µM SNAP prior to partnering with zebrafish embryos having been subjected to irradiation with an X-ray dose of 75 mGy. These findings revealed the importance of NO in the protection against damages induced by ionizing radiations or by radiation-induced bystander signals, and could have important impacts on development of advanced cancer treatment strategies.

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“…In the present work, zebrafish ( Danio rerio ) embryos were employed as the in vivo vertebrate model to assess the effect of photon hormesis on dose responses to alpha particles. Zebrafish embryos had been commonly used in research related to toxicology screening and ionizing radiation [27,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50] considering their numerous advantages, including their rapid development process, high fecundity, optically transparency and, most importantly, their genomes sharing considerable homology with human genomes [51]. It was established that different alpha-particle doses would lead to hormetic and toxic effects in zebrafish embryos [49], so a dose response curve for graded alpha-particle dose was established in the present work to fully assess the effect of photon hormesis.…”

Section: Introductionmentioning

confidence: 99%

Effect of Photon Hormesis on Dose Responses to Alpha Particles in Zebrafish Embryos

Cheng

2017

IJMS

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Photon hormesis refers to the phenomenon where the biological effect of ionizing radiation with a high linear energy transfer (LET) value is diminished by photons with a low LET value. The present paper studied the effect of photon hormesis from X-rays on dose responses to alpha particles using embryos of the zebrafish (Danio rerio) as the in vivo vertebrate model. The toxicity of these ionizing radiations in the zebrafish embryos was assessed using the apoptotic counts at 20, 24, or 30 h post fertilization (hpf) revealed through acridine orange (AO) staining. For alpha-particle doses ≥ 4.4 mGy, the additional X-ray dose of 10 mGy significantly reduced the number of apoptotic cells at 24 hpf, which proved the presence of photon hormesis. Smaller alpha-particle doses might not have inflicted sufficient aggregate damages to trigger photon hormesis. The time gap T between the X-ray (10 mGy) and alpha-particle (4.4 mGy) exposures was also studied. Photon hormesis was present when T ≤ 30 min, but was absent when T = 60 min, at which time repair of damage induced by alpha particles would have completed to prevent their interactions with those induced by X-rays. Finally, the drop in the apoptotic counts at 24 hpf due to photon hormesis was explained by bringing the apoptotic events earlier to 20 hpf, which strongly supported the removal of aberrant cells through apoptosis as an underlying mechanism for photon hormesis.

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Embryos of the zebrafish Danio rerio in studies of non-targeted effects of ionizing radiation

Cited by 27 publications

References 218 publications

Zebrafish as an In Vivo Model to Assess Epigenetic Effects of Ionizing Radiation

Zebrafish as an In Vivo Model to Assess Epigenetic Effects of Ionizing Radiation

Exogenous Nitric Oxide Suppresses in Vivo X-ray-Induced Targeted and Non-Targeted Effects in Zebrafish Embryos

Effect of Photon Hormesis on Dose Responses to Alpha Particles in Zebrafish Embryos

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