Embryos derived from the in vitro fertilization of oocytes of pregnant cows

Vajta, Gábor; Macháty, Zoltán; Bárándi, Zs.; Varga, Zsolt

doi:10.1016/0093-691x(92)90043-q

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Prepubertal animals yield large numbers of oocytes per ovary (Thonon et al 1993) and cows under three years of age provide oocytes with a significantly higher developmental capacity than elderly animals (Mermillod et al 1992b). The stage of the oestrous cycle has apparently no effect on oocyte quality nor the number of follicles present (Tan and Lu 1990), and pregnancy does not affect oocyte quality whatsoever (Vajta et al 1992). However, individual differences in oocyte quality between donor cows have been reported (Berg and Brem 1991;Mermillod et al 1992b;Van Soom et al 1994b).…”

Section: In Vifromentioning

confidence: 96%

Oocyte Maturation, Sperm Capacitation and Pre‐implantation Development in the Bovine: Implications for in vitro Production of Embryos

Soom

Kruif

1996

Reprod Domestic Animals

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Section: In Vifromentioning

confidence: 96%

Oocyte Maturation, Sperm Capacitation and Pre‐implantation Development in the Bovine: Implications for in vitro Production of Embryos

Soom

Kruif

1996

Reprod Domestic Animals

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In Vitro Fertilization in Hungarian Grey Cattle

Varga

Bárádi

Macháty

et al. 1993

Reprod Domestic Animals

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Contents Experiments have been performed to investigate the potential use of in vitro fertilization for the preservation of the endangered Hungarian Grey cattle. 1018 excellent quality oocytes from 167 ovaries of slaughtered Hungarian Grey cows and heifers were matured. Out of 966 oocytes cocultivated with pre‐test and swim‐up selected Hungarian Grey frozen‐thawed semen, 386 (39.9%) cleaved and 141 (14.6%) reached the morulablastocyst stage on the 7th day of embryo culture (1.69 transferable embryos per animal). Some embryos were transferred into the uterine horn of 10 natural cycling Hereford, Hungarian Red‐spotted, Hungarian Red‐spotted x Hereford recipients. Sixty days after transfer, 4 recipients were diagnosed as being pregnant by ultrasonography (two multiple births are expected); since then one calf has been born. Our results indicate that the IVF method can be used for the preservation of endangered cattle breeds, reducing the losses caused by slaughter for various reasons. Inhalt: In vitro Befruchtung beim ungarischen Grauvieh Es wurden Experimente durchgeführt, um die Möglichkeiten zu erforschen, die vom Aussterben bedrohte Rinderrasse Ungarisches Grauvieh über in vitro Befruchtung zu bewahren. 1018 Eizellen sehr guter Qualität wurden von 167 Ovarien geschlachteter Ungarischer Grauer Kühe und Färsen gereift. Von diesen wurden 966 Oozyten mittels vorgeprüftem und mit swim‐up selektiertem Grauvieh‐Sperma nach dem Auftauen kokultiviert. 386 (39,9%) der Oozyten teilten sich und 141 (14,6%) erreichten das Morula‐ bzw. Blastocystenstadium nuch 7tägiger Embryokultur (1,69 transferierbare Embryonen per Tier). Einige Embryonen wurden in das Uterushorn von 10 normal zyklischen Hereford, Ungarischem Rothvieh und der Kreuzungsrasse aus beiden übertragen. Sechzig Tage nach dem Transfer wurden 4 Tiere per Ultraschalluntersuchung als tragend diagnostiziert (zwei Mehrlingsträchtigkeiten wurden erwartet). Bisher ist ein Kalb geboren. Unsere Ergebnisse zeigen, daβ es möglich ist, mittels in vitro Befnrchtung bedrohte Ramen, wie das ungarische Grauvieh, zu erhalten und die Schlachtverluste, die aus verschiedenen Gründen möglich sein mögen, zu reduzieren.

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