Embryonic Versus Adult Stem Cells

Procházková, Michaela; Chavez, Miquella G.; Procházka, Jan; Felfy, Hady; Mushegyan, Vagan; Klein, Ophir D.

doi:10.1016/b978-0-12-397157-9.00020-5

Cited by 26 publications

(13 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…While ESCs can become all cell types, ASCs have a limited differentiation potential. The primary roles of ASCs are to maintain tissue homeostasis and to some extent, to replace cells that die due to injury or disease [ 133 , 134 , 135 , 136 ].…”

Section: Organoids From Embryonic Stem Cells (Escs)mentioning

confidence: 99%

The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update

Azar

Bahmad

Daher

et al. 2021

IJMS

View full text Add to dashboard Cite

Organoids represent one of the most important advancements in the field of stem cells during the past decade. They are three-dimensional in vitro culturing models that originate from self-organizing stem cells and can mimic the in vivo structural and functional specificities of body organs. Organoids have been established from multiple adult tissues as well as pluripotent stem cells and have recently become a powerful tool for studying development and diseases in vitro, drug screening, and host–microbe interaction. The use of stem cells—that have self-renewal capacity to proliferate and differentiate into specialized cell types—for organoids culturing represents a major advancement in biomedical research. Indeed, this new technology has a great potential to be used in a multitude of fields, including cancer research, hereditary and infectious diseases. Nevertheless, organoid culturing is still rife with many challenges, not limited to being costly and time consuming, having variable rates of efficiency in generation and maintenance, genetic stability, and clinical applications. In this review, we aim to provide a synopsis of pluripotent stem cell-derived organoids and their use for disease modeling and other clinical applications.

show abstract

Section: Organoids From Embryonic Stem Cells (Escs)mentioning

confidence: 99%

The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update

Azar

Bahmad

Daher

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Organoids can be generated from adult stem cells (ASCs) ( 3 , 4 ) or pluripotent stem cells (PSCs) such as embryonic stem cells (ESCs) ( 5 , 6 ) and induced pluripotent stem cells (iPSCs) ( 7 , 8 ). Both ASCs and ESCs are undifferentiated cells that are either present among differentiated cells in a tissue or organ or derived from the inner cell mass of blastocysts, respectively ( 9 ). iPSCs are derived from differentiated somatic cells which are converted to regain pluripotency ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

“…Both ASCs and ESCs are undifferentiated cells that are either present among differentiated cells in a tissue or organ or derived from the inner cell mass of blastocysts, respectively ( 9 ). iPSCs are derived from differentiated somatic cells which are converted to regain pluripotency ( 9 ). While ASCs can only differentiate into distinct cell types of their original tissue, ESCs and iPSCs can differentiate into all cell types within the body ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans

Kawasaki

Goyama

Tachibana

et al. 2022

Front. Med. Technol.

View full text Add to dashboard Cite

Animal organoid models derived from farm and companion animals have great potential to contribute to human health as a One Health initiative, which recognize a close inter-relationship among humans, animals and their shared environment and adopt multi-and trans-disciplinary approaches to optimize health outcomes. With recent advances in organoid technology, studies on farm and companion animal organoids have gained more attention in various fields including veterinary medicine, translational medicine and biomedical research. Not only is this because three-dimensional organoids possess unique characteristics from traditional two-dimensional cell cultures including their self-organizing and self-renewing properties and high structural and functional similarities to the originating tissue, but also because relative to conventional genetically modified or artificially induced murine models, companion animal organoids can provide an excellent model for spontaneously occurring diseases which resemble human diseases. These features of companion animal organoids offer a paradigm-shifting approach in biomedical research and improve translatability of in vitro studies to subsequent in vivo studies with spontaneously diseased animals while reducing the use of conventional animal models prior to human clinical trials. Farm animal organoids also could play an important role in investigations of the pathophysiology of zoonotic and reproductive diseases by contributing to public health and improving agricultural production. Here, we discuss a brief history of organoids and the most recent updates on farm and companion animal organoids, followed by discussion on their potential in public health, food security, and comparative medicine as One Health initiatives. We highlight recent evolution in the culturing of organoids and their integration with organ-on-a-chip systems to overcome current limitations in in vitro studies. We envision multidisciplinary work integrating organoid culture and organ-on-a-chip technology can contribute to improving both human and animal health.

show abstract

“…Embryonic stems cells are pluripotent and may confer an advantage over MSCs, BMSCs and TSPCs. Furthermore, ESCs can be harvested and grown relatively easily and for longer durations in cell culture (13).…”

Section: Introductionmentioning

confidence: 99%

Stem cell secretome, regeneration, and clinical translation: a narrative review

Gwam¹,

Mohammed²,

Ma³

2021

Ann Transl Med

View full text Add to dashboard Cite

Regenerative medicine is a field growing in popularity due to high hopes for stimulating in situ tissue restoration. Stem cell therapy remain at the center of regenerative medicine, due to early reports on its pluripotent differentiating capability. However, more recent reports suggest the paracrine activity of stem cells, and not direct differentiation, as the cause of its therapeutic effects. This paracrine activity can be harnessed in the form of conditioned media. Despite these capabilities, the clinical translation of stem cell conditioned media (i.e., secretome) is precluded by a variety of factors. These limitations include standardization of stem cell-conditioned media formulation, characterization of bioactive factors in conditioned media and dosing, optimizing modes of delivery, and uncovering of mechanisms of action of stem cell conditioned media. The purpose of this review is to provide a focused narration on the aforementioned preclusions pertaining to the clinical translation of stem cell conditioned media. Specifically, we will report on commonly use methodologies for the development of stem cell conditioned media, modalities for conditioned media characterization, modes of delivery, and postulated mechanisms of action for stem cell conditioned media in regenerative medicine.

show abstract

Embryonic Versus Adult Stem Cells

Cited by 26 publications

References 98 publications

The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update

The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update

Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans

Stem cell secretome, regeneration, and clinical translation: a narrative review

Contact Info

Product

Resources

About