Embryonic sympathoblasts transiently express TrkB in vivo and proliferate in response to brain-derived neurotrophic factor in vitro

Straub, Jennifer A; Sholler, Giselle L. Saulnier; Nishi, Rae

doi:10.1186/1471-213x-7-10

Cited by 20 publications

(10 citation statements)

References 32 publications

(32 reference statements)

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“…In addition, Wetmore and Olson 21 show BDNF protein expression in sympathetic ganglia although BDNF mRNA is absent. In contrast, primary sympathetic neurons have been shown to express TrkB mRNA 21 22 .…”

Section: Resultsmentioning

confidence: 99%

“…Previous reports of Trk b −/− or B dnf −/− mice failed to show a defect in sympathetic ganglia, however, these reports used superior cervical ganglia for their investigations (somite levels 5–10; not thoracic/trunk sympathetic ganglia levels at somite 18 19 20 21 22 23 24 34 35 36 ). Furthermore, TrkB expression has previously been reported in lumbar sympathetic ganglia but not superior cervical ganglia 22 , further indicating the superior cervical ganglia is not a good predictor of trunk sympathetic ganglia behavior. Finally, BDNF has been reported to be expressed by mouse sympathetic ganglia at E14.5, however, this equates to chick day 7, well after sympathetic ganglia dorsal migration is complete 37 .…”

Section: Discussionmentioning

confidence: 98%

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TrkB/BDNF signalling patterns the sympathetic nervous system

et al. 2015

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The sympathetic nervous system is essential for maintaining mammalian homeostasis. How this intricately connected network, composed of preganglionic neurons that reside in the spinal cord and post-ganglionic neurons that comprise a chain of vertebral sympathetic ganglia, arises developmentally is incompletely understood. This problem is especially complex given the vertebral chain of sympathetic ganglia derive secondarily from the dorsal migration of ‘primary' sympathetic ganglia that are initially located several hundred microns ventrally from their future pre-synaptic partners. Here we report that the dorsal migration of discrete ganglia is not a simple migration of individual cells but a much more carefully choreographed process that is mediated by extensive interactions of pre-and post-ganglionic neurons. Dorsal migration does not occur in the absence of contact with preganglionic axons, and this is mediated by BDNF/TrkB signalling. Thus BDNF released by preganglionic axons acts chemotactically on TrkB-positive sympathetic neurons, to pattern the developing peripheral nervous system.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

TrkB/BDNF signalling patterns the sympathetic nervous system

et al. 2015

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“…Upon binding to their ligands, NGF, brain-derived neurotrophic factor (BDNF), neurotro-phin-3, respectively, these receptors regulate proliferation, survival, and differentiation in normal neuronal cells (Brodeur et al , 2009; Maris and Matthay, 1999; Maris et al , 2007; Straub et al , 2007). These receptors all associate with p75, a low affinity receptor that may enhance the binding of ligand to the Trk proteins or alter the function of the Trk receptors (Brodeur et al , 2009; Maris and Matthay, 1999; Maris et al , 2007; Straub et al , 2007). High levels of TrkA, in association with very low/no N-myc expression, are detected in favorable NB tumors which often spontaneous regress (Nakagawara, 1993; Nakagawara et al , 1992).…”

Section: The Role Of Neurotrophins and Growth Factors In The Develmentioning

confidence: 99%

“…A more detailed discussion on the temporal expression of the transcription factors in the sym-pathoadreanal lineage (including developmental stages) can be found in (Howard et al , 2000) and details about the distinct chromaffin and sympathetic lineages can be found in (Ernsberger et al , 2005). Additional of neurotrophins and their receptors can be found in (Straub et al , 2007). Transcription Factors are shown in bold and factors implicated in neuroblastoma have been underlined.…”

Section: Figure 4mentioning

confidence: 99%

The Connections Between Neural Crest Development and Neuroblastoma

Jiang

Stanke

Lahti

2011

Current Topics in Developmental Biology

127

132

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Neuroblastoma (NB), the most common extracranial solid tumor in childhood, is an extremely heterogeneous disease both biologically and clinically. Although significant progress has been made in identifying molecular and genetic markers for NB, this disease remains an enigmatic challenge. Since NB is thought to be an embryonal tumor that is derived from precursor cells of the peripheral (sympathetic) nervous system, understanding the development of normal sympathetic nervous system may highlight abnormal events that contribute to NB initiation. Therefore, this review focuses on the development of the peripheral trunk neural crest, the current understanding of how developmental factors may contribute to NB and on recent advances in the identification of important genetic lesions and signaling pathways involved in NB tumorigenesis and metastasis. Finally, we discuss how future advances in identification of molecular alterations in NB may lead to more effective, less toxic therapies, and improve the prognosis for NB patients.

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“…Dentre os produtos celulares, pode-se ressaltar a síntese de moléculas de adesão celular, como laminina, fibronectina e colágeno, importantes para o cultivo e o crescimento celular [5,6] . Além disso, alguns experimentos in vitro demonstraram que a utilização de fatores de crescimento e outras substâncias como o interferon e acetato de glatiramer provocam a proliferação celular [7,8] . Por outro lado, as características intrínsecas dos biomateriais, como a especificidade química da superfície, elétrica, hidrofobicidade e topografia, podem influenciar na adesão e crescimento celular [1,3,4,9,10] .…”

Section: Introductionunclassified

Estudo das células Neuro2A sobre os biomateriais PCL e PLLA

et al. 2014

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Resumo: Os biomateriais poli L-ácido lático (PLLA) e o poli caprolactona (PCL) são os polímeros mais estudadas na área dos materiais bioreabsorvíveis. Dentre as suas principais características que contribuem para a interação celular, temos a especificidade química da superfície, elétrica, hidrofobicidade e topografia. Ainda, observa-se o tempo de degradação, porosidade, biocompatibilidade com o tecido biológico, bem como, a confecção com as mais variadas formas e dimensões. Já a prática da cultura celular, tem como objetivo estudar a adesão, migração, diferenciação e a proliferação celular utilizando-se um determinado material ou substância. Contudo, poucos trabalhos utilizando os biomateriais ora supracitados e a aplicação em células neuro2A foram realizados. Sabe-se que este tipo celular é derivado de células embrionárias da crista neural, as quais originam em neurônios simpáticos e apresentam como característica a imortalidade, portanto, são excelentes modelos em ensaios in vitro. Nesse sentido, o presente estudo avalia a adesão e a proliferação desta linhagem celular sobre os biopolímeros poli caprolactona (PCL) e poli L-ácido lático (PLLA). Palavras-chave: Biomateriais, PLLA, PLC, neuroblastoma, cultura. Study of Neuro2a Cells on the Biomaterials Poly L-lactic Acid and Poly CaprolactoneAbstract: Biomaterials poly L-lactic acid (PLLA) and poly caprolactone (PCL) are the most studied in the area of bioresorbable materials. Among the main features that contribute to cell interaction, we have the specific surface chemistry, electrical, hydrophobicity and topography. Also, is observed the degradation time, porosity, biocompatibility with biological tissue, as well as the preparation of the most varied shapes and sizes. The practice of cell culture, aims to study the adhesion, migration, differentiation and cell proliferation using a given material or substance. However, few studies were performed using these biomaterials and the application of neuro2A cells. It is known that this cell type is derived from the embryonic neural crest cells, which originate in sympathetic neurons and have the characteristic of immortality, therefore, are excellent models in vitro assays. Accordingly, the present study evaluates the adhesion and proliferation of this cell line on the biopolymers poly caprolactone (PCL) and poly L-lactic acid (PLLA). Glória, 187, Centro, Diamantina, MG, Brasil, Keywords: Biomaterials, PLLA, PLC, neuroblastoma, culture. Autor para correspondência: Amauri Pierucci, Departamento de Ciências Básicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri -UFVJM, Rua da IntroduçãoCom o advento da bioengenharia intensificaram os estudos sobre a aplicação e ação dos biomateriais em diferentes tipos celulares. Sabe-se que a adesão, migração, diferenciação e a proliferação celular sobre determinado substrato depende em parte das propriedades intrínsecas de cada tipo celular, bem como, o tipo de material utilizado [1][2][3][4] . Dentre os produtos celulares, pode-se ressaltar a síntese de moléculas de adesã...

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Embryonic sympathoblasts transiently express TrkB in vivo and proliferate in response to brain-derived neurotrophic factor in vitro

Cited by 20 publications

References 32 publications

TrkB/BDNF signalling patterns the sympathetic nervous system

TrkB/BDNF signalling patterns the sympathetic nervous system

The Connections Between Neural Crest Development and Neuroblastoma

Estudo das células Neuro2A sobre os biomateriais PCL e PLLA

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