Embryonic Stem Cells in Toxicological Studies

Estevan, Carmen; Romero, Andrea C.; Pamies, David; Vilanova, E.; Sogorb, Miguel A.

doi:10.5772/24804

Cited by 6 publications

(2 citation statements)

References 78 publications

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“…In addition, recent investigations suggest that human embryos are extremely sensitive to RA exposure and, despite its biological activities, both an excess and deficiency of RA may result in birth defects. Moreover, the complexity of human embryos generates misunderstandings as to the exact role of RA in embryo toxicity and since the animal studies are very expensive, time-consuming, and are associated with many ethical concerns [17,18], fetal-derived stem cells such as HUCMSCs can provide a simple and reliable fetal model for cellular assays including embryo toxicity tests [19]. The study described here is involved in determining whether RA might have an apoptotic effect on HUCMSCs.…”

Section: Introductionmentioning

confidence: 99%

Biological parameters influencing the human umbilical cord‐derived mesenchymal stem cells' response to retinoic acid

et al. 2014

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Human umbilical cord-derived mesenchymal stem cells (HUCMSCs) are multipotent fetal stem cells that differentiate into various cell lineages. In recent years, they have gained attention for therapeutic applications but very little is known about their sensitivity to chemical agents such as widely used retinoic acid (RA). As a morphogen inducing differentiation of mesenchymal stem cells, RA has for a long time been known to be a potent teratogen promoting craniofacial and limb abnormality in vertebrate embryos. Here, using MTT assay and EB/AO staining as well as TUNEL assay we show that RA in a concentration-dependent manner induces apoptosis through upregulating Caspase expression and increasing Bax/Bcl2 ratio. Moreover, different biological parameters such as initial time seeding, cell density, passage number and duration of RA treatment play a major role in HUCMSCs cytotoxic response to this agent.

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Section: Introductionmentioning

confidence: 99%

Biological parameters influencing the human umbilical cord‐derived mesenchymal stem cells' response to retinoic acid

et al. 2014

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“…This is an important function for these cells. Stem cells are very vulnerable to the toxicity of ambient chemicals during embryogenesis, which is a phase of rapid cellular division 78 . This might lead to a deleterious influence on the development of both the embryo and the foetus if the toxins are toxic enough.…”

Section: Molecular and Biological Tests Based On Stem Cells For The M...mentioning

confidence: 99%

A Comprehensive Review on Zebrafish and Zebrafish Embryonic Stem Cells (Zescs) as the Versatile Biotechnological Green Tool for Detecting Environmental Pollutants

Christy.L,

Nellore

2023

Biosci., Biotech. Res. Asia

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This article discusses the embryonic development of zebrafish, which are essential aquatic models for investigating neurotoxicity caused by environmental toxins. Zebrafish are one of the few fish species that can survive in highly toxic environments, making them an interesting model for assessing pollutants' performance and determining their toxicity. Zebrafish's limited life expectancy, ease of maintenance and growth, transparent embryos, and homology of genetic and biological features make them an interesting tool for studying the effects of common substances like insecticides, nanoparticles, and food supplements on human health. The study focuses on the regulation of morphology and behavior, stress caused by oxidative damage, transcription, neurogenesis, and neuron progression. Further research is needed at cellular and signaling levels to understand the detrimental processes of pollutants in relation to epigenetic toxicity, negative interference of the BB barrier, and manipulation of the brain-gut-microbiota axis. Environmental pollution is a significant issue that poses a severe threat to public health. Biotechnological principles are now being widely used to monitor environmental pollutants due to their versatile applications. In this regard, zebrafish has established as a prominent vertebrate prototype organism, which offers a unique platform for toxicity screening and efficacy testing of various chemicals. Studies have shown that exposure to toxicants during embryonic development can cause developmental, cardiovascular, and neurodevelopmental toxicity, as well as hepatic disorders in zebrafish. Therefore, zebrafish has been extensively used to study the neurotoxicity of environmental pollutants, including pesticides, nanoparticles, food additives, and other pollutants. Additionally, the review discusses the use of zebrafish-derived embryonic stem cells (ZESCs) for environmental pollutants monitoring. Stem cells are highly sensitive to the toxicity of environmental chemicals during embryogenesis, which can lead to adverse effects on embryonic and fetal development. Thus, ZESCs cultivation and utilization in toxicological assays have become a valuable tool for evaluating the toxicity of potential environmental toxicants. The application of green science principles to evaluate the toxicity of environmental pollutants using zebrafish and ZESCs has enormous potential. This approach offers a sustainable and environmentally friendly way to monitor pollutants, and it can contribute to the development of effective mitigation strategies. Overall, the use of zebrafish and ZESCs in biotechnological pollutants monitoring could have far-reaching effects for public health and environmental sustainability.

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Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology

Easley

2019

Methods in Molecular Biology

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Embryonic Stem Cells in Toxicological Studies

Cited by 6 publications

References 78 publications

Biological parameters influencing the human umbilical cord‐derived mesenchymal stem cells' response to retinoic acid

Biological parameters influencing the human umbilical cord‐derived mesenchymal stem cells' response to retinoic acid

A Comprehensive Review on Zebrafish and Zebrafish Embryonic Stem Cells (Zescs) as the Versatile Biotechnological Green Tool for Detecting Environmental Pollutants

Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology

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