Embryonic stem cells derived from morulae, inner cell mass, and blastocysts of mink: Comparisons of their pluripotencies

Sukoyan, M. A.; Vatolin, Sergei; Golubitsa, A. N.; Zhelezova, A. I.; Semenova, Lora A.; Serov, O. L.

doi:10.1002/mrd.1080360205

Cited by 101 publications

(57 citation statements)

References 15 publications

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“…After three passages, cells were transferred onto the STO derivative SNL 76/7 cell line [10] and maintained on this immortalized mouse cell line for all subsequent passages. Passaged clumps of cells gave rise to flat colonies that differed in appearance from mouse and human ES cell colonies but were morphologically similar to that reported for mink ES cells [11][12][13][14]. A phase-contrast image of a canine ES cell colony is shown in Figure 2A.…”

Section: Es Cell Derivationsupporting

confidence: 71%

Derivation, Characterization, and In Vitro Differentiation of Canine Embryonic Stem Cells

et al. 2007

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Canine embryonic stem (cES) cell lines were generated to establish a large-animal preclinical model for testing the safety and efficacy of embryonic stem (ES) cell-derived tissue replacement therapy. Putative cES cell lines were initiated from canine blastocysts harvested from natural matings. Times of harvest were estimated as 12-16 days after the presumed surge in circulating levels of luteinizing hormone. Four lines established from blastocysts harvested at days 13-14 postsurge satisfied most of the criteria for embryonic stem cells, whereas lines established after day 14 did not. One line, Fred Hutchinson dog (FHDO)-7, has been maintained through 34 passages and is presented here. FHDO-7 cells are alkaline phosphatase-positive and express both message and protein for the Oct4 transcription factor. They also express message for Nanog and telomerase but do not express message for Cdx2, which is associated with trophectoderm. Furthermore, they express a cluster of pluripotency-associated microRNAs (miRs) (miR-302b, miR-302c, and miR-367) characteristic of human and mouse ES cells. The FHDO-7 cells grow on feeder layers of modified mouse embryonic fibroblasts as flat colonies that resemble ES cells from mink, a close phylogenetic relative of dog. When cultured in nonadherent plates without feeders, the cells form embryoid bodies (EBs). Under various culture conditions, the EBs give rise to ectoderm-derived neuronal cells expressing ␥-enolase and ␤3-tubulin; mesoderm-derived cells producing collagen IIA1, cartilage, and bone; and endoderm-derived cells expressing ␣-fetoprotein or Clara cell-specific protein.

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Section: Es Cell Derivationsupporting

confidence: 71%

Derivation, Characterization, and In Vitro Differentiation of Canine Embryonic Stem Cells

et al. 2007

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“…Cultures of EC or ES-like cells have been developed from mink (Sukoyan et al, 1993), hamster (Doetschman et al, 1988), rat (Iannaccone et al, 1994), pig (Wheeler, 1994), bovine (First et al, 1994), sheep (Piedrahita et al, 1990), chicken (Pain et al, 1996), and monkey (Thomson et al, 1995). Although these cell lines share several features with murine ES cells, for most of them evidence of developmental capacity in vivo is lacking; none have been able to colonize the germ line of recipient hosts.…”

Section: Es Cells In Mouse and Non-piscine Animal Speciesmentioning

confidence: 99%

Fish ES Cells and Applications to Biotechnology

et al. 2006

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ES cells provide a promising tool for the generation of transgenic animals with site-directed mutations. When ES cells colonize germ cells in chimeras, transgenic animals with modified phenotypes are generated and used either for functional genomics studies or for improving productivity in commercial settings. Although the ES cell approach has been limited to mice, there is strong interest for developing the technology in fish. We describe the step-by-step procedure for developing ES cells in fish. Key aspects include avoiding cell differentiation, specific in vitro traits of pluripotency, and, most importantly, testing for production of chimeric animals as the main evidence of pluripotency. The entire process focuses on two model species, zebrafish and medaka, in which most work has been done. The achievements attained in these species, as well as their applicability to other commercial fish, are discussed. Because of the difficulties relating to germ line competence, mostly of long-term fish ES cells, alternative cell-based approaches such as primordial germ cells and nuclear transfer need to be considered. Although progress to date has been slow, there are promising achievements in homologous recombination and alternative avenues yet to be explored that can bring ES technology in fish to fruition.

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“…The isolation of ES-like cells from other species, i.e. chicken (Pain et al 1996), mink (Sukoyan et al 1993), hamster (Doetschman et al 1988), rat (Iannaccone et al 1994), pig (Wheeler, 1994;Shim et al 1997), cow (First et al 1994), rhesus monkey (Thomson et al 1995), rabbit (Graves & Moreadith, 1993) and recently human (Thomson et al 1998) was attempted. Although these ES-like cells share common features with murine ES cells, they have not yet been shown to reconstitute the germ line.…”

mentioning

confidence: 99%