Embryonic stem cell markers expression in cancers

Schoenhals, Matthieu; Kassambara, Alboukadel; Vos, John De; Hose, Dirk; Moreaux, Jérôme; Klein, Bernard

doi:10.1016/j.bbrc.2009.02.156

Cited by 219 publications

(218 citation statements)

References 43 publications

(32 reference statements)

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“…This gene is expressed at high levels in neuroepithelial stem cells and its expression persists in neural stem/progenitor cells throughout adulthood (Miyagi et al, 2006). Sox2 is highly expressed in ES and EC cells (Perrett et al, 2008;Schoenhals et al, 2009) and several studies have focused on modulating its expression in these cells in order to elucidate the role of this transcription factor in stem cell pluripotency and self-renewal (Zhao et al, 2004;Boer et al, 2007;Masui et al, 2007;Kopp et al, 2008;Adachi et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones

Drakulić¹,

Krstić²,

Stevanović³

2012

Genet. Mol. Res.

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ABSTRACT. SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and pluripotency of stem cells, we developed and characterized SOX2-overexpressing NT2/D1 cell clones. Using Southern blot and semiquantitative RT-PCR, we confirmed integration and expression of exogenous SOX2 in three NT2/D1 cell clones. Overexpression of the SOX2 gene was detected in two of these clones. SOX2 overexpression in NT2/D1 cell clones resulted in altered expression of key pluripotency genes OCT4 and NANOG. Furthermore, SOX2-overexpressing NT2/D1 cell clones entered into retinoic acid-dependent neural differentiation, even when there was elevated SOX2 expression. After 21 days of induction by retinoic acid, expression of neural markers (neuroD1 and synaptophysin) was higher in induced cell clones than in induced parental cells. The cell clone with SOX2 overexpression had an approximately 1.3-fold higher growth rate compared to parental cells. SOX2 overexpression did not increase the population of cells undergoing apoptosis. Taken together, we developed two SOX2- overexpressing cell clones, with constitutive SOX2 expression after three weeks of retinoic acid treatment. SOX2 overexpression resulted in altered expression of pluripotency-related genes, increased proliferation, and altered expression of neural markers after three weeks of retinoic acid treatment.

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Section: Discussionmentioning

confidence: 99%

Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones

Drakulić¹,

Krstić²,

Stevanović³

2012

Genet. Mol. Res.

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“…5 Furthermore, after undergoing the differentiation therapy of Yin et al, 1 the gene expression levels of c-Myc and Klf4 became lower than that of Oct3/4 and Sox2. This was comparable to the cases of human iPS cells.…”

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confidence: 99%

The reprogramming factors of human somatic cells are novel targets for human hepatocellular carcinoma therapy

Moriguchi

2009

Hepatology

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“…Notably, KLF4 collaborating with other transcription factors including Oct4, Sox2 and c-Myc, drives somatic cells into iPS cells. CSCs have been identified in various tumors, and KLF4 can be speculated to have similar functions in CSCs based on its function in ES cell [133]. Our work provides evidence for the first time that KLF4 is essential for the maintenance of breast CSC and cell migration and invasion, which may be helpful to develop new therapeutic strategies for breast cancer.…”

Section: Discussionmentioning

confidence: 63%