Embryonic germ cell extracts erase imprinted genes and improve the efficiency of induced pluripotent stem cells

Hu, Jing; Zhao, Qiaoshi; Feng, Yukuan; Li, Na; Gu, Yanli; Sun, Ruizhen; Duan, Lian; Wu, Yanshuang; Shan, Zhiyan; Lei, Lei

doi:10.1038/s41598-018-29339-0

Cited by 9 publications

(8 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…mediated by cell-secreted factors 10,16,17 . Consistently with this hypothesis, we recently reported that the efficiency of reprogramming of human somatic cells to hiPSCs can be dramatically improved in a microfluidic confined environment 18 , which enhances the accumulation of secreted factors 19,20 and sustains the acquisition of both primed 18,21 and naïve human pluripotency 22 .…”

Section: Introductionsupporting

confidence: 57%

See 1 more Smart Citation

Cellular population dynamics shape the route to human pluripotency

Elvassore

Gagliano

Panariello

et al. 2022

Preprint

View full text Add to dashboard Cite

Human cellular reprogramming to induced pluripotency is still an inefficient process and this has long hindered the study of the role of critical intermediate stages. We take advantage of high efficiency reprogramming in microfluidics and temporal multi-omics to identify and resolve distinct sub-populations and their interactions. The combination of secretome analysis and single-cell transcriptomics shows functional extrinsic pathways of protein communication between reprogramming sub-populations and the re-shaping of a permissive extracellular environment. We pinpointed the HGF/MET/STAT3 axis as a potent enhancer of reprogramming, which acts via HGF accumulation within the confined system of microfluidics, and in conventional dishes needs to be supplied exogenously to enhance efficiency. Our data integrate the notion of human cellular reprogramming as a transcription factor-driven process, with the concept that it is deeply dependent on extracellular context and cell population determinants.

show abstract

Section: Introductionsupporting

confidence: 57%

“…Secretome analysis during reprogramming. To investigate the dynamic changes of cell-secreted proteins during reprogramming of human fibroblasts, tandem mass spectrometry (LC-MS/MS) was performed on conditioned media pooled from microfluidic channels every 2 days [19][20][21] (Fig. 1B).…”

Section: Resultsmentioning

confidence: 99%

Cellular population dynamics shape the route to human pluripotency

Elvassore

Gagliano

Panariello

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…OCT-3/4 is regulated by PTM processes that are involved in phosphorylation [33,34], SUMOylation [35], ubiquitination [1,36,37], glycosylation [38], methylation [39][40][41], and acetylation [42] (Figure 2).…”

Section: Oct-3/4 Regulated By Several Ptmsmentioning

confidence: 99%

“…proposed that combining three transcription factors, NANOG, OCT-3/4, and TET1, regulates DNA methylation modification, which governs pluripotency through reprogramming [39]. Differentiation-induced de novo DNA demethylation can repress pluripotency genes including OCT-3/4, whereas active DNA demethylation reactivates pluripotency genes [41].…”

Section: Oct-3/4 Regulated By Several Ptmsmentioning

confidence: 99%

Cellular Functions of OCT-3/4 Regulated by Ubiquitination in Proliferating Cells

Baek

Choi

Pei

2020

Cancers

View full text Add to dashboard Cite

Octamer-binding transcription factor 3/4 (OCT-3/4), which is involved in the tumorigenesis of somatic cancers, has diverse functions during cancer development. Overexpression of OCT-3/4 has been detected in various human somatic tumors, indicating that OCT-3/4 activation may contribute to the development and progression of cancers. Stem cells can undergo self-renewal, pluripotency, and reprogramming with the help of at least four transcription factors, OCT-3/4, SRY box-containing gene 2 (SOX2), Krüppel-like factor 4 (KLF4), and c-MYC. Of these, OCT-3/4 plays a critical role in maintenance of undifferentiated state of embryonic stem cells (ESCs) and in production of induced pluripotent stem cells (iPSCs). Stem cells can undergo partitioning through mitosis and separate into specific cell types, three embryonic germ layers: the endoderm, the mesoderm, and the trophectoderm. It has been demonstrated that the stability of OCT-3/4 is mediated by the ubiquitin-proteasome system (UPS), which is one of the key cellular mechanisms for cellular homeostasis. The framework of the mechanism is simple, but the proteolytic machinery is complicated. Ubiquitination promotes protein degradation, and ubiquitination of OCT-3/4 leads to regulation of cellular proliferation and differentiation. Therefore, it is expected that OCT-3/4 may play a key role in proliferation and differentiation of proliferating cells.

show abstract

“…3/ Treating permeabilized cells with cell extracts from pluripotent cells – Permeabilized differentiated cells are partially reprogrammed when exposed to protein extracts prepared from pluripotent (ESC) cells or germ cells [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Nucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view

Fulka

Loi

Palazzese

et al. 2022

Journal of Reproduction and Development

View full text Add to dashboard Cite

It is now approximately 25 years since the sheep Dolly, the first cloned mammal where the somatic cell nucleus from an adult donor was used for transfer, was born. So far, somatic cell nucleus transfer, where G1-phase nuclei are transferred into cytoplasts obtained by enucleation of mature metaphase II (MII) oocytes followed by the activation of the reconstructed cells, is the most efficient approach to reprogram/remodel the differentiated nucleus. In general, in an enucleated oocyte (cytoplast), the nuclear envelope (NE, membrane) of an injected somatic cell nucleus breaks down and chromosomes condense. This condensation phase is followed, after subsequent activation, by chromatin decondensation and formation of a pseudo-pronucleus (i) whose morphology should resemble the natural postfertilization pronuclei (PNs). Thus, the volume of the transferred nuclei increases considerably by incorporating the content released from the germinal vesicles (GVs). In parallel, the transferred nucleus genes must be reset and function similarly as the relevant genes in normal embryo reprogramming. This, among others, covers the relevant epigenetic modifications and the appropriate organization of chromatin in pseudo-pronuclei. While reprogramming in SCNT is often discussed, the remodeling of transferred nuclei is much less studied, particularly in the context of the developmental potential of SCNT embryos. It is now evident that correct reprogramming mirrors appropriate remodeling. At the same time, it is widely accepted that the process of rebuilding the nucleus following SCNT is instrumental to the overall success of this procedure. Thus, in our contribution, we will mostly focus on the remodeling of transferred nuclei. In particular, we discuss the oocyte organelles that are essential for the development of SCNT embryos.

show abstract

Embryonic germ cell extracts erase imprinted genes and improve the efficiency of induced pluripotent stem cells

Cited by 9 publications

References 50 publications

Cellular population dynamics shape the route to human pluripotency

Cellular population dynamics shape the route to human pluripotency

Cellular Functions of OCT-3/4 Regulated by Ubiquitination in Proliferating Cells

Nucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view

Contact Info

Product

Resources

About