Embryonic exposure to low concentrations of aflatoxin B1 triggers global transcriptomic changes, defective yolk lipid mobilization, abnormal gastrointestinal tract development and inflammation in zebrafish

Ivánovics, Bence; Gazsi, Gyöngyi; Reining, Marta; Berta, Izabella; Póliska, Szilárd; Tóth, Márta; Domokos, Apolka; Nagy, Béla; Staszny, Ádám; Cserháti, Mátyás; Csősz, Éva; Bácsi, Attila; Csenki-Bakos, Zsolt; Ács, András; Urbányi, Béla; Czimmerer, Zsolt

doi:10.1016/j.jhazmat.2021.125788

Cited by 22 publications

(10 citation statements)

References 89 publications

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“…current study positively confirms the earlier findings of Wangikar et al (2004), Shuaib et al (2010), Fetaih et al (2014, Smith et al (2017), Ivanovics et al (2021) and Xu et al (2021) in this aspect. Abdulrazzaq et al (2002Abdulrazzaq et al ( , 2004) demonstrated that the harmful effects of AFs on fetuses are caused by maternal consumption of contaminated food during pregnancy.…”

Section: Discussionsupporting

confidence: 93%

“…The body weights of pregnant females showed a considerable reduction and teratogenic changes in the fetuses, represent the impacts and damage. Ivanovics et al (2021) found that in domesticated animals and humans, AFB1 contaminated feeds and meals caused a variety of health concerns, including tumors formation and hepatotoxicity. Exposure to AFs may result in negative pregnancy outcomes such as baby growth impairment, preterm delivery and pregnancy loss (Shuaib et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

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Mycotoxicity and embryonic development: I- Aflatoxin B1 reduces quality and birth rate during mice embryonic development

Ali

Khalil

Hassan

et al. 2023

SVU-International Journal of Veterinary Sciences

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Mycotoxins are fungal products often found in food, formed during growth, harvesting, drying or storage of fruits, seeds, or grains, leading to a variety of toxic effects in humans and animals. Aflatoxins are a family of polyketide secondary metabolite produced by the food-contaminating moulds Aspergillus parasiticus and Aspergillus flavus. Aflatoxin B1 (AFB1) is a carcinogenic, teratogenic, mutagenic and growth inhibitory mycotoxin. To explore the negative morphological effects of AFB1 on the developing mice embryo, 50 adult females albino mice (CD1) were divided into five groups (10 females for each group): Control, positive control and three groups treated with a daily oral dose of 5, 10 or 20 μg/kg bw of AFB1 extract. Fifteen days after treatments, females were caged with males. Pregnancy, mortality, embryos number rates and body weight of female mice and infants were recorded. At 21 days old, crown rump, head, ear, tail, fore and hind-limb lengths of mice infants were investigated. Current data showed a decrease in pregnancy and embryo number rate among females in the animals treated with 10 & 20 μg AFB1, while treatment with 5 μg showed an increase in pregnancy and embryo number rate compared to control one, while mortality rate among fetuses increased in the three experimental animals treated with AFB1. Some cases of abortion were observed in 10 & 20 μg groups, while ulcers were observed in 20 μg groups. Weight and all morphometric parameters showed significant decrease except tail length that showed insignificant increase in the treated groups compared to the control one, while ear length showed significant increase. Different congenital malformations were observed in infants such as tail loss, delayed hair and teeth development and poor ossification in treated groups. The present study demonstrated that AFB1 induced developmental anomalies and reduced quality and birth rate. Data clarified that minimum concentration of AFB1 required to induce teratogenicity is far beyond the minimum concentrations estimated in earlier literatures. It is suggested that AFB1 might change genetic elements and consequently interfere with bone mineralization and induce teratogenicity and anomalies during mice embryonic development. Further studies are required to explore the mechanism of action.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Mycotoxicity and embryonic development: I- Aflatoxin B1 reduces quality and birth rate during mice embryonic development

Ali

Khalil

Hassan

et al. 2023

SVU-International Journal of Veterinary Sciences

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“…The semiconducting polymer PPy [24] was blended with zein to confer electrical properties to the electrospun nanofibers. The platform was used to detect aflatoxin B1 (AFB1), a metabolite produced by Aspergillus flavus and Aspergillus parasitic, a common food-contaminating mycotoxin which threats humans and animals due to its carcinogenic, teratogenic, and immunosuppressive nature [25][26][27]. This contaminant is resistant to thermal and chemical treatment, being found in such food products as corn, barley, nut, bean, oils, rice, fruits, and milk [28,29].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Immunosensor Made with Zein‐based Nanofibers for On‐site Detection of Aflatoxin B1

et al. 2022

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A disposable electrochemical immunosensor for on-site detection of aflatoxin B1(AFB1), one of the most toxic mycotoxins in agri-food products, was fabricated through a low-cost cut-printing method and then modified with zein/polypyrrole(PPy) electrospun nanofibers onto which anti-AFB1 monoclonal antibodies were immobilized covalently. Fabrication was possible with an innovative and simple approach to adsorb nanofibers onto the working electrode during electrospinning. Electrochemi-cal impedance spectroscopy was employed as the principle of detection, and the data collected with a portable potentiostat were treated with information visualization techniques. The nanostructured immunosensor showed a high sensitivity for AFB1 with a linear detection range from 0.25 to 10 ng mL À 1 and a theoretical limit of detection of 0.092 ng mL À 1 , which is adequate to detect AFB1 in food, according to regulatory agencies.

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“…It was reported that anti-inflammatory cytokines in the skin, spleen, and head kidney of fish are regulated by the TOR signaling pathway (12,29). Ivanovics et al (30) found that AFB1 exposure significantly reduced the arginine level in zebrafish larvae lysates. Chen et al (31) reported that arginine deficiency downregulated TOR mRNA expression in Jian carp (Cyprinus carpio var.…”

Section: Introductionmentioning

confidence: 99%

“…Ivanovics et al. ( 30 ) found that AFB1 exposure significantly reduced the arginine level in zebrafish larvae lysates. Chen et al.…”

Section: Introductionmentioning

confidence: 99%

Dietary Aflatoxin B1 attenuates immune function of immune organs in grass carp (Ctenopharyngodon idella) by modulating NF-κB and the TOR signaling pathway

Zeng

et al. 2022

Front. Immunol.

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Aflatoxin B1 (AFB1) is kind of a common mycotoxin in food and feedstuff. Aquafeeds are susceptible to contamination of AFB1. In teleost fish, the spleen and head kidney are key immune organ. Moreover, the fish skin is a critical mucosal barrier system. However, there was little study on the effects of dietary AFB1 on the immune response of these immune organs in fish. This study aimed to explore the impacts of oral AFB1 on the immune competence and its mechanisms in the skin, spleen, and head kidney of grass carp. Our work indicated that dietary AFB1 reduced antibacterial compounds and immunoglobulins contents, and decreased the transcription levels of antimicrobial peptides in grass carp immune organs. In addition, dietary AFB1 increased the transcription levels of pro-inflammatory cytokines and reduced the transcription levels of anti-inflammatory cytokines in the grass carp immune organs, which might be regulated by NF-κB and TOR signaling, respectively. Meanwhile, we evaluated the content of AFB1 in the grass carp diet should not exceed 29.48 μg/kg diet according to the levels of acid phosphatase and lysozyme. In summary, dietary AFB1 impaired immune response in grass carp skin, spleen, and head kidney.

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Embryonic exposure to low concentrations of aflatoxin B1 triggers global transcriptomic changes, defective yolk lipid mobilization, abnormal gastrointestinal tract development and inflammation in zebrafish

Cited by 22 publications

References 89 publications

Mycotoxicity and embryonic development: I- Aflatoxin B1 reduces quality and birth rate during mice embryonic development

Mycotoxicity and embryonic development: I- Aflatoxin B1 reduces quality and birth rate during mice embryonic development

Electrochemical Immunosensor Made with Zein‐based Nanofibers for On‐site Detection of Aflatoxin B1

Dietary Aflatoxin B1 attenuates immune function of immune organs in grass carp (Ctenopharyngodon idella) by modulating NF-κB and the TOR signaling pathway

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