Embryonic Explant and Plumular Meristem Transformation Methods for Development of Transgenic Pigeon Pea

Ganguly, Shreeparna; Purohit, Arnab; Chaudhuri, Rituparna Kundu; Das, Sampa; Chakraborti, Dipankar

doi:10.1007/978-1-0716-0235-5_17

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Non-chimeric lines are further selected in the T 1 generation. (B) Plumular meristem approach [22,148]. In the plumular meristem approach, young seedlings are decapitated and their radicules excised with a sterile scalpel.…”

Section: Means Of In Planta Transformationmentioning

confidence: 99%

“…Amongst the different protocols using direct de novo shoot organogenesis, the plumular meristem strategy was proposed as a time-efficient direct regenerationbased transformation approach with high transformation rates for chickpea [22,146] and pigeon pea [147,148]. In this system, three-day-old seedlings are decapitated at the shoot apex and pricked in the apical portion and cotyledonary nodes [22,147] (Fig.…”

Section: Plumular Meristem Strategymentioning

confidence: 99%

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A comprehensive review of in planta stable transformation strategies

Bélanger,

Copley,

Hoyos-Villegas

et al. 2024

Plant Methods

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Plant transformation remains a major bottleneck to the improvement of plant science, both on fundamental and practical levels. The recalcitrant nature of most commercial and minor crops to genetic transformation slows scientific progress for a large range of crops that are essential for food security on a global scale. Over the years, novel stable transformation strategies loosely grouped under the term “in planta” have been proposed and validated in a large number of model (e.g. Arabidopsis and rice), major (e.g. wheat and soybean) and minor (e.g. chickpea and lablab bean) species. The in planta approach is revolutionary as it is considered genotype-independent, technically simple (i.e. devoid of or with minimal tissue culture steps), affordable, and easy to implement in a broad range of experimental settings. In this article, we reviewed and categorized over 300 research articles, patents, theses, and videos demonstrating the applicability of different in planta transformation strategies in 105 different genera across 139 plant species. To support this review process, we propose a classification system for the in planta techniques based on five categories and a new nomenclature for more than 30 different in planta techniques. In complement to this, we clarified some grey areas regarding the in planta conceptual framework and provided insights regarding the past, current, and future scientific impacts of these techniques. To support the diffusion of this concept across the community, this review article will serve as an introductory point for an online compendium about in planta transformation strategies that will be available to all scientists. By expanding our knowledge about in planta transformation, we can find innovative approaches to unlock the full potential of plants, support the growth of scientific knowledge, and stimulate an equitable development of plant research in all countries and institutions.

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Section: Means Of In Planta Transformationmentioning

confidence: 99%

Section: Plumular Meristem Strategymentioning

confidence: 99%

A comprehensive review of in planta stable transformation strategies

Bélanger,

Copley,

Hoyos-Villegas

et al. 2024

Plant Methods

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Tackling Lentil Biotic Stresses in the Genomic Era

Vega

Garcı́a

Gutiérrez-González

et al. 2022

Genomic Designing for Biotic Stress Resistant Pulse Crops

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Lentils are already one of the main pulses of the world, as they are one of the main sources of protein for humans. As a crop, they are also gaining momentum because the rusticity and tolerance to water scarcity of some varieties are a good fit with the current global warming trend and climate change in general. However, while the harvested area and overall production have drastically increased over the last decades, yield has only experienced very modest increments. The reasons are two-fold. First, pathogens are affecting the crop as never before, likely due to not only the changing climate but also to the expansion of lentil cultivation to new geographic areas. Second, genomics-aided breeding is far behind many other crops. This is in partly due to the lack of genomic tools currently available to researchers. Progress is being made to adopt high-throughput genomic methods, and researchers will be able to tackle lentil gene discovery and breeding for pathogen resistance and other biotic stresses more efficiently in the coming years. We outline the current situation, novel findings, and prospects of lentil research for biotic stresses. IntroductionLentil (Lens culinaris Medik. subsp. culinaris) is one of the first domesticated species in the Fertile Crescent and along with barley, emmer wheat and einkorn wheat, pea, chickpea, and flax was part of the set of crops that defined the beginnings of the Neolithic transition to agriculture in this part of the World. The origin of the cultivated form is the wild L. culinaris subsp. orientalis (Boiss.) Ponert (syn. L. orientalis Boiss.). A recent publication by Liber et al. (2021) suggests that phylogenetics, population structure, and archeological data coincide in a lentil domestication prolonged in time in Southwest Asia, with two different domesticated gene pools. From the

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Embryonic Explant and Plumular Meristem Transformation Methods for Development of Transgenic Pigeon Pea

Cited by 2 publications

References 12 publications

A comprehensive review of in planta stable transformation strategies

A comprehensive review of in planta stable transformation strategies

Tackling Lentil Biotic Stresses in the Genomic Era

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