Embryonic development of the pig: Ultrastructure and uptake of macromolecules during early pregnancy

Barends, P.M.G.; Taverne, Nico; Stroband, H.W.J.; Blommers, P.C.J.

doi:10.1016/0309-1651(85)90004-9

Cited by 2 publications

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“…Specific staining for uteroferrin was found in trophectoderm, but not in endoderm or the inner cell mass. This is in agreement with results of marker protein uptake and consistent with ultrastructural organelle distribution in mouse (Fleming and Goodall 1986) and pig (Barends et al 1985;Stroband et al 1984) blastocysts. Endosomes and secondary lysosomes were restricted to trophectoderm and not to inner cell mass.…”

Section: Discussionsupporting

confidence: 80%

“…Labeling was intense in the supranuclear cytoplasm, the site of the Golgi apparatus (Dantzer 1985;Geisert et al 1982a;Stroband et al 1984). Other workers (Barends et al 1985;Stroband et al 1984) have shown that the pre-implantation pig blastocyst (day 8-11) internalizes horseradish peroxidase or cationized ferritin at the apical membrane in coated pits, pinocytotic vesicles, endosomes and secondary lysosomes. The puncrate distribution in the cytoplasm probably represents vesicular uptake of FITC-labeled uteroferrin.…”

Section: Discussionmentioning

confidence: 90%

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Immunolocalization and endocytosis of the uterine secretory protein, uteroferrin, in pre-implantation pig trophectoderm on day 11 of pregnancy

et al. 1990

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Uteroferrin is a progesterone-induced, iron-binding glycoprotein secreted by the glandular epithelium of the pig endometrium. Evidence is presented that maternal uteroferrin is present in trophectoderm of preimplantation pig blastocysts on day 11 of pregnancy. Although [35S]-methionine was not incorporated into uteroferrin during in vitro culture of blastocysts, solubilized tissue extracts from 10-20-mm-diameter blastocysts contained uteroferrin by western blotting with monospecific antiserum to uteroferrin. Uteroferrin was detected in the apical and basolateral cytoplasm of trophectoderm by immunocytochemistry of paraffin-embedded blastocysts. Immunostaining was excluded from cells of the endoderm and the inner cell mass. Furthermore, blastocysts internalized fluorescein-labeled uteroferrin from medium during in vitro culture in a temperature-dependent manner. Fluorescent label was located in apical and basolateral cytoplasm in a punctate distribution, and clustered in the supranuclear region of trophectoderm. Addition of a threefold excess of unlabeled uteroferrin to culture medium did not inhibit uptake. These results suggest that the pre-implantation pig blastocyst actively endocytoses uteroferrin from glandular secretions in utero. Uptake was restricted to trophectoderm.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 90%

Immunolocalization and endocytosis of the uterine secretory protein, uteroferrin, in pre-implantation pig trophectoderm on day 11 of pregnancy

et al. 1990

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The ultrastructure of the uterine epithelium of the pig during the estrous cycle and early pregnancy

et al. 1986

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The ultrastructure of the endometrial epithelium of the pig was studied during the estrous cycle and early pregnancy up to implantation. Special attention was given to the luminal epithelium and morphological indications of protein synthesis. Although the general morphology of the luminal and glandular epithelia is similar (both tissues consist of secretory cells and ciliated cells at all the stages studied), it appears that the two epithelia should be considered as two functionally different units in the pre-implantation period. Morphological evidence suggests the presence of at least three different secretory products within luminal epithelial cells; they are released at different times, i.e. at estrus, between day 8 and 10 and after day 11. The glandular epithelium shows release of secretory products from day 10-11. Increasing amounts of glycogen were found within epithelial cells, especially in pregnant gilts from day 12. The possible significance of secretory activity of the epithelium is discussed in relation to the development of the embryos.

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Embryonic development of the pig: Ultrastructure and uptake of macromolecules during early pregnancy

Cited by 2 publications

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Immunolocalization and endocytosis of the uterine secretory protein, uteroferrin, in pre-implantation pig trophectoderm on day 11 of pregnancy

Immunolocalization and endocytosis of the uterine secretory protein, uteroferrin, in pre-implantation pig trophectoderm on day 11 of pregnancy

The ultrastructure of the uterine epithelium of the pig during the estrous cycle and early pregnancy

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