Embryonic Development of the Epidermis

Kashgari, Ghaidaa; Huang, Yanzhi; Andersen, Bogi

doi:10.1016/b978-0-12-801238-3.65811-7

Cited by 2 publications

(4 citation statements)

References 157 publications

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“…6E). While SE marker TFAP2C decreased, simple epithelial markers KRT8 and KRT18 (30) increased over the course of the differentiation. Moreover, TP63, the master regulator of keratinocyte differentiation (69), was up-regulated (Fig.…”

Section: Cell Density Directs Cell Fate Decisions Between Amniotic An...mentioning

confidence: 96%

“…S2). Furthermore, they were marked by the cells expressing SE marker genes, GRHL3 and MSX1 (30,34,37,(44)(45)(46), and therefore were annotated as SE. Two large clusters featured by enhanced expression of AE genes (0 and 2) were annotated as AE (Fig.…”

Section: Single-cell Rna Sequencing Analysis On Abcp Culture Reveals ...mentioning

confidence: 99%

“…On the other hand, surface ectoderm (SE) is a continuous dense sheet of epidermal progenitors, which begin to appear around gastrulation and eventually give rise to the skin and epithelia of the eye, mouth and nasal cavity, as well as their appendages (29). SE originates from a subpopulation of ectoderm that is set apart from neural commitment by the action of bone morphogenetic protein (BMP) signaling, whereas ectoderm derives from pluripotent epiblast that is neither exposed to fibroblast growth factor (FGF) nor NODAL signaling, both of which promote commitment to mesoderm and endoderm (30,31). Recently, single-cell RNA sequencing (scRNA-seq) analyses revealed that AE and SE largely share the expression of nonneural ectoderm (NNE) genes (24,32).…”

Section: Introductionmentioning

confidence: 99%

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Human surface ectoderm and amniotic ectoderm are sequentially specified according to cellular density

Nakanoh,

Sham,

Ghimire

et al. 2024

Sci. Adv.

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Mechanisms specifying amniotic ectoderm and surface ectoderm are unresolved in humans due to their close similarities in expression patterns and signal requirements. This lack of knowledge hinders the development of protocols to accurately model human embryogenesis. Here, we developed a human pluripotent stem cell model to investigate the divergence between amniotic and surface ectoderms. In the established culture system, cells differentiated into functional amnioblast-like cells. Single-cell RNA sequencing analyses of amnioblast differentiation revealed an intermediate cell state with enhanced surface ectoderm gene expression. Furthermore, when the differentiation started at the confluent condition, cells retained the expression profile of surface ectoderm. Collectively, we propose that human amniotic ectoderm and surface ectoderm are specified along a common nonneural ectoderm trajectory based on cell density. Our culture system also generated extraembryonic mesoderm–like cells from the primed pluripotent state. Together, this study provides an integrative understanding of the human nonneural ectoderm development and a model for embryonic and extraembryonic human development around gastrulation.

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Section: Cell Density Directs Cell Fate Decisions Between Amniotic An...mentioning

confidence: 96%

Section: Single-cell Rna Sequencing Analysis On Abcp Culture Reveals ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human surface ectoderm and amniotic ectoderm are sequentially specified according to cellular density

Nakanoh,

Sham,

Ghimire

et al. 2024

Sci. Adv.

View full text Add to dashboard Cite

show abstract

“…EdU staining of forelimb sections shows active cell proliferation along the basal epidermis, including in (although not preferentially) the IET (Figure 3D). The IET is reminiscent of other developmental structures involved in collective migration of epithelia (Kashgari et al, 2018;Scarpa and Mayor, 2016), suggesting that it may play an active role in the separation of the digits.…”

Section: Formation Of An Iet In Normal Digit Separationmentioning

confidence: 99%