Embryonic cardiomyocyte expression of endothelial genes

Welikson, Robert E.; Kaestner, Stefanie; Evans, Angela; Hauschka, Stephen D.

doi:10.1002/dvdy.21276

Cited by 6 publications

(4 citation statements)

References 57 publications

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“…It also acts as a marker of cardiac stem/progenitor cells [39,40]. In this study, early beating areas and cells isolated from these areas also expressed this protein.…”

Section: Discussionmentioning

confidence: 86%

Enhancement of cardiomyocyte differentiation from human embryonic stem cells

Wang

Chen

Song

et al. 2010

Sci. China Life Sci.

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Several approaches have been used to encourage the differentiation of cardiomyocytes from human embryonic stem cells. However, the differentiation efficiency is low, and appropriate culture protocols are needed to produce adequate numbers of cardiomyocytes for therapeutic cell transplantation. This study investigated the effects of serum on cardiomyocyte differentiation in suspension culture medium during embryoid body (EB) formation by human embryonic stem cells. The addition of ascorbic acid, dimethylsulfoxide and 5-aza-2'-deoxycytidine during days 5-7 at the EB-forming stage resulted in an increase in the numbers of rhythmically contracting clusters of derived cardiomyocytes. Treatment with 0.1 mmol L(-1) ascorbic acid alone, or more notably in combination with 10 micromol L(-1) 5-aza-2'-deoxycytidine, induced the formation of beating cells within EBs. Most of the beating clusters had spontaneous contraction rates similar to those found in human adults, and their contractile activity lasted for up to 194 days.

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“…It also acts as a marker of cardiac stem/progenitor cells [39,40]. In this study, early beating areas and cells isolated from these areas also expressed this protein.…”

Section: Discussionmentioning

confidence: 86%

Enhancement of cardiomyocyte differentiation from human embryonic stem cells

Wang

Chen

Song

et al. 2010

Sci. China Life Sci.

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“…Endothelial cell marker is an important indicator of microvascular structures and healthy and functional myocardium tissue. [ 48 ] After an injury, the infarcted area of the myocardium is usually replenished with fibroblasts, resulting in the loss of cardiomyocytes and microvascular, leading to the dysfunction of the myocardium. [ 49 ] As demonstrated in Figure 7f, a large portion of the infarcted region was covered with vWF positive staining cells (green) in the Gel‐E treatment group, indicating activation of angiogenesis.…”

Section: Resultsmentioning

confidence: 99%

Injection‐Free Delivery of MSC‐Derived Extracellular Vesicles for Myocardial Infarction Therapeutics

Tang

Cui

Zhang

et al. 2021

Adv Healthcare Materials

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As emerging therapeutic factors, extracellular vesicles (EVs) offer significant potential for myocardial infarction (MI) treatment. Current delivery approaches for EVs involve either intra-myocardial or intravenous injection, where both have inherent limitations for downstream clinical applications such as secondary tissue injury and low delivery efficiency. Herein, an injection-free approach for delivering EVs onto the heart surface to treat MI is proposed. By spraying a mixture of EVs, gelatin methacryloyl (GelMA) precursors, and photoinitiators followed by visible light irradiation for 30 s, EVs are physically entrapped within the GelMA hydrogel network covering the surface of the heart, resulting in an enhanced retention rate. Moreover, EVs are gradually released from the hydrogel network through a combination of diffusion and/or enzymatic degradation of the hydrogel, and they are effectively taken up by the sprayed tissue area. More importantly, the released EVs further migrate deep into myocardium tissue, which exerts an improved therapeutic effect. In an MI-induced mice model, the group treated with EVs-laden GelMA hydrogels shows significant recovery in cardiac function after 4 weeks. The work demonstrates a new strategy for delivering EVs into cardiac tissues for MI treatment in a localized manner with high retention.

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“…These EPDCS proliferate but remain joined by tight junctions, showing a cobblestone-like appearance ( Fig 1B and 1C and S1 Fig ). The heart separated from the EPDCs contains primarily cardiomyocytes (>90%), a small fraction (~6%) of endothelial cells, and even smaller fraction of other cell types [ 22 ]. Hence the predominant cellular players in the co-culture system are EPDCs and the myocardium.…”

Section: Resultsmentioning

confidence: 99%

Proteomic characterization of epicardial-myocardial signaling reveals novel regulatory networks including a role for NF-κB in epicardial EMT

Urban

Midura

et al. 2017

PLoS ONE

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Signaling between the epicardium and underlying myocardium is crucial for proper heart development. The complex molecular interactions and regulatory networks involved in this communication are not well understood. In this study, we integrated mass spectrometry with bioinformatics to systematically characterize the secretome of embryonic chicken EPDC-heart explant (EHE) co-cultures. The 150-protein secretome dataset established greatly expands the knowledge base of the molecular players involved in epicardial-myocardial signaling. We identified proteins and pathways that are implicated in epicardial-myocardial signaling for the first time, as well as new components of pathways that are known to regulate the crosstalk between epicardium and myocardium. The large size of the dataset enabled bioinformatics analysis to deduce networks for the regulation of specific biological processes and predicted signal transduction nodes within the networks. We performed functional analysis on one of the predicted nodes, NF-κB, and demonstrate that NF-κB activation is an essential step in TGFβ2/PDGFBB-induced cardiac epithelial-to-mesenchymal transition. In summary, we have generated a global perspective of epicardial-myocardial signaling for the first time, and our findings open exciting new avenues for investigating the molecular basis of heart development and regeneration.

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Embryonic cardiomyocyte expression of endothelial genes

Cited by 6 publications

References 57 publications

Enhancement of cardiomyocyte differentiation from human embryonic stem cells

Enhancement of cardiomyocyte differentiation from human embryonic stem cells

Injection‐Free Delivery of MSC‐Derived Extracellular Vesicles for Myocardial Infarction Therapeutics

Proteomic characterization of epicardial-myocardial signaling reveals novel regulatory networks including a role for NF-κB in epicardial EMT

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