Embryogenic calli induced in interspecific (Elaeis guineensis x E. oleifera) hybrid zygotic embryos

Angelo, P. C. da S.; Lopes, Ricardo; Moraes, L. A. C.; Cunha, Raimundo Nonato Vieira da

doi:10.12702/1984-7033.v09n03a10

Cited by 4 publications

(3 citation statements)

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“…Also, adventitious shoot apical meristem surrounded by leaf primordia was observed and distinguished by presence of vascular connection with the maternal tissue ( Figure 6H1) as shoot organogenesis. The results of the current study are on line with those of Angelo et al (2009), Demeter et al ( 2010), Elviana et al ( 2011) and Steinmacher et al (2011) who reported that protodermis was a unique character and the first tissue identified somatic embryogenesis development .…”

Section: Histological Analysissupporting

confidence: 91%

Morpho- histological observations on somatic embryogenesis in mature embryo derived callus of Oryza sativa L. cv. Sakha 101

Sakr

Sayed

2018

Journal of Scientific Research in Science

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Cellular totipotency is one of the fundamental principles of plant biotechnology. The mature embryo is increasingly recorded as a valuable explant for somatic embryogenesis in rice biotechnology. In present study, rice cells dedifferentiation, proliferation and redifferentiation were investigated by culturing mature rice (Oryza sativa L. cv. Sakha 101) embryos in modified MS media fortified with different growth regulators alone and in combination. Mature embryo tissues competent for tissue culture and the chronological changes of cells morphology and histology were observed. The results showed that callus was induced only from mature rice seed (explant) cultured on MS media supplemented with 2, 4-D (2 or 2.5 mg/l) alone while the rest treatments showed negative response. Callus was initiated after 5 days of culture in MS media fortified with the lower 2, 4-D dose as clusters of undifferentiated cell masses while callus initiation was delayed 4 days more by increasing the applied dose. At morphological level, Pale yellowish and friable calli was noted in both tested doses. Calli texture exhibited different appearance, while was slightly nodular in the lower dose it was soft in the higher one. High callus induction frequency (70%) was estimated for 2mg/l 2, 4-D application while decreased frequency (40%) was concomitant with dose increment. Histological analysis for somatic embryogenesis revealed that within two weeks of culturing explants on callus induction medium (CIM), somatic embryos development began as clusters of embryonic cells at the peripheral parts of the proliferated calli while nonembryonic cells were observed at the inner regions of the induced callus. Embryogenic cells at the outer cell layer were observed as small and isodiametric with dense cytoplasm and clear nucleus located in the center of the cells, whereas the non embryogenic cells were large, vacuolated and had a very small nucleus located near the cell wall. Embryogenic cells undergo series of ordered divisions and protodermis observed surrounding globular embryo was recorded at the end of culturing in CIM fortified with lower 2, 4-D dose. On the other hand, culture on MS fortified with higher dose delayed rice cells differentiation and globular stage was recorded two days after subculture embryonic callus into free hormone MS medium. After 2 days of subculture into free hormone MS medium, heart shaped embryo was observed in low dose of 2, 4-D. This study assists to draw attention to the use of a histological approach as a helpful tool to follow the chronological series of embryo development in vitro.

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Section: Histological Analysissupporting

confidence: 91%

Morpho- histological observations on somatic embryogenesis in mature embryo derived callus of Oryza sativa L. cv. Sakha 101

Sakr

Sayed

2018

Journal of Scientific Research in Science

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“…The induction of shoots on poly-embryogenic complexes was proven successful [12,13] as proposed earlier for the interspecific hybrids [45]. Cultivating poly-embryogenic complexes in medium supplemented with abscisic acid [9] before shoot induction might contribute to synchronization [46].…”

Section: Discussionmentioning

confidence: 79%

Histological analysis and transcription profiles on somatic embryogenesis in interspecific hybrids of <i>Elaeis guineensis × E. oleifera</i>

Angelo¹,

Steinmacher²,

Lopes³

et al. 2013

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“…In addition, in a genetic improvement program, cloning from ZE can accelerate culture selection cycles. This is especially true in backcross generations, where each embryo represents a unique genotype, for permitting the replication of experiments in different locations or conditions, reinforcing the representation of the environment throughout the multiple steps of the selection process (Angelo et al 2009;Balzon et al 2013).…”

Section: Introductionmentioning

confidence: 99%

An improved protocol for somatic embryogenesis and plant regeneration in macaw palm (Acrocomia aculeata) from mature zygotic embryos

Luis

Scherwinski‐Pereira

2014

Plant Cell Tiss Organ Cult

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An improved protocol for plant regeneration via somatic embryogenesis was developed using mature macaw palm (Acrocomia aculeata) zygotic embryos as initial explant. For induction of the embryogenic callus (EC), two basic media (BM) were tested consisting of Murashige and Skoog and Eeuwens (Y3) salts with 30 g L-1 sucrose, 0.5 g L-1 glutamine and 2.5 g L-1 Phytagel. The 3,6-dichloro-2-methoxybenzoic acid (dicamba), 4-amino-3,5,6-trichloro-picolinic acid (picloram) and 2,4-dichlorophenoxyacetic acid (2,4-D) auxins were added to the culture media at concentrations of 0, 1.5 or 3.0 mg L-1. After 240 days, the embryogenic calli were transferred to the respective BM media with auxin concentrations reduced to 0.5 or 1.0 mg L-1 in order to differentiate the somatic embryos (SEs). Plant regeneration was performed on the BM media without growth regulators. Embryogenic calli were observed after 180 days of culture and in all treatments with auxin. The Y3 medium showed the best EC formation results (60.8 %). These calli showed yellowish coloration, compact consistency and nodular aspect. After 60 days in differentiation medium, SEs were verified in different stages of development. Histological analysis showed that the SEs were formed from a nodular EC. The SEs generally presented unicellular origin with suspensor formation, and at the end of development, bipolar embryos were observed. The plant regeneration frequency reached levels up to 31.9 % when using induction medium consisting of Y3 associated to 1.5 mg L-1 of 2,4-D and the subsequent auxin reduction to 0.5 mg L-1 in the differentiation stage. Regenerated plants showed normal development, with root and aerial part growth.

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Embryogenic calli induced in interspecific (Elaeis guineensis x E. oleifera) hybrid zygotic embryos

Abstract: -The hybridization between oil palm (Elaeis guineensis)

Cited by 4 publications

References 6 publications

Morpho- histological observations on somatic embryogenesis in mature embryo derived callus of Oryza sativa L. cv. Sakha 101

Morpho- histological observations on somatic embryogenesis in mature embryo derived callus of Oryza sativa L. cv. Sakha 101

Histological analysis and transcription profiles on somatic embryogenesis in interspecific hybrids of <i>Elaeis guineensis × E. oleifera</i>

An improved protocol for somatic embryogenesis and plant regeneration in macaw palm (Acrocomia aculeata) from mature zygotic embryos

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Embryogenic calli induced in interspecific (Elaeis guineensis x E. oleifera) hybrid zygotic embryos

Abstract: -The hybridization between oil palm (Elaeis guineensis)

Cited by 4 publications

References 6 publications

Morpho- histological observations on somatic embryogenesis in mature embryo derived callus of Oryza sativa L. cv. Sakha 101

Morpho- histological observations on somatic embryogenesis in mature embryo derived callus of Oryza sativa L. cv. Sakha 101

Histological analysis and transcription profiles on somatic embryogenesis in interspecific hybrids of &lt;i&gt;Elaeis guineensis &#215; E. oleifera&lt;/i&gt;

An improved protocol for somatic embryogenesis and plant regeneration in macaw palm (Acrocomia aculeata) from mature zygotic embryos

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Histological analysis and transcription profiles on somatic embryogenesis in interspecific hybrids of <i>Elaeis guineensis × E. oleifera</i>