Embryogenesis in Quercus petraea

Jörgensen, Jörg

doi:10.1051/forest:19930738

Cited by 19 publications

(10 citation statements)

References 7 publications

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“…Somatic embryos were obtained only on MS medium supplemented with 0-4.5 lM 2,4-D, whereas Gingas and Lineberger (1989) obtained somatic embryos of Q. rubra using immature zygotic embryos cultured on modified MS medium containing various combinations of 2,4-D and BA, with the highest number of embryos induced on plant growth regulator-free MS medium under a 16 h light photoperiod. Zygotic embryos cultured on modified WPM supplemented with 0.5-5.0 lM BA and 2.5-10.0 lM 2,4-D developed somatic embryos in Q. petraea (Jörgensen 1993). Similarly, 2,4-D and BA were used to initiate somatic embryos from immature and mature zygotic embryos in other Quercus species (Hübner et al 1995;Manzanera et al 1996;Endemann and Wilhelm 1999).…”

Section: Resultsmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.)

Vengadesan

Pijut

2009

Plant Cell Tiss Organ Cult

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A somatic embryogenesis protocol for plant regeneration of northern red oak (Quercus rubra) was established from immature cotyledon explants. Embryogenic callus cultures were induced on Murashige and Skoog medium (MS) containing 3% sucrose, 0.24% Phytagel TM , and various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) after 4 weeks of culture in darkness. A higher response (66%) of embryogenic callus was induced on 0.45 lM 2,4-D. Higher numbers of globular-(31), heart-(17), torpedo-(12), and cotyledon-stage (8) embryos per explant were obtained by culturing embryogenic callus on MS with 3% sucrose, 0.24% Phytagel TM , and devoid of growth regulators after 8 weeks culture in darkness. Continuous sub-culturing of embryogenic callus on medium containing 2,4-D yielded only compact callus. Desiccation of embryos for 3 days in darkness at 25 ± 2°C followed by cold storage at 4°C in darkness for 8 weeks favored embryo germination and development of plantlets. Cotyledon-stage embryos subjected to desiccation and chilling treatment cultured on MS with 3% sucrose, 0.24 Phytagel TM , 0.44 lM 6-benzylaminopurine (BA), and 0.29 lM gibberellic acid germinated at a higher frequency (61%) than with 0.44 lM BA alone and control cultures. Germinated plantlets developed a shoot and root, were acclimatized successfully, and maintained in a growth room for plantlet development.

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Section: Resultsmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.)

Vengadesan

Pijut

2009

Plant Cell Tiss Organ Cult

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“…Recently, the storage in liquid nitrogen of small Q. petraea embryos has been reported (Jorgensen 1993).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Quercus spp. (Oak): In Vitro Culture and Production of Tannins

Favre¹,

Scalbert

Penhoat

1993

Biotechnology in Agriculture and Forestry

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“…Embryogenesis has been achieved in tissues from floral parts of several woody species (Teixeira et al 1994;Carimi et al 1995;Alemanno et al 1996;Cote et al 1996;Capuana and Debergh 1997;Merkle et al 1998), including some species of the genus Quercus (Gingas 1991;Jörgensen 1993;Bueno et al 1997). Some of these studies were aimed at obtaining haploids through gametic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Vegetative propagation of Quercus suber L. by somatic embryogenesis

2003

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Somatic embryogenesis was induced in expanding leaves from epicormic shoots forced to sprout from segments of branches collected from several hundredyear-old cork oak trees. Following a basic protocol previously defined for leaves taken from seedlings of this species, several factors were studied to improve the response. The induction frequency was significantly higher when the length of exposure to growth regulators was increased from 7 to 30 days. The combined application of NAA and BAP was essential for induction. Although both regulators had a very significant influence, their interaction was not significant, suggesting independent roles. Leaf size had a crucial effect, because beyond a certain threshold, embryogenesis could not be obtained. Embryogenic lines were maintained via repetitive embryogenesis on hormone-free medium for more than 2 years.

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Embryogenesis in Quercus petraea

Cited by 19 publications

References 7 publications

Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.)

Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.)

Quercus spp. (Oak): In Vitro Culture and Production of Tannins

Vegetative propagation of Quercus suber L. by somatic embryogenesis

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